Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01250 and RR42_RS01255 are separated by 100 nucleotides RR42_RS01255 and RR42_RS01260 overlap by 4 nucleotides RR42_RS01260 and RR42_RS01265 are separated by 385 nucleotides
RR42_RS01250: RR42_RS01250 - diaminopimelate epimerase, at 282,646 to 283,512
_RS01250
RR42_RS01255: RR42_RS01255 - lauroyl acyltransferase, at 283,613 to 284,506
_RS01255
RR42_RS01260: RR42_RS01260 - lauroyl acyltransferase, at 284,503 to 285,441
_RS01260
RR42_RS01265: RR42_RS01265 - S-adenosylmethionine synthetase, at 285,827 to 286,990
_RS01265
Position (kb) 284
285
286 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 283.523 kb on + strand at 283.523 kb on + strand at 283.617 kb on + strand at 283.617 kb on + strand at 283.669 kb on - strand at 283.672 kb on - strand at 283.785 kb on + strand, within RR42_RS01255 at 283.785 kb on + strand, within RR42_RS01255 at 283.795 kb on - strand, within RR42_RS01255 at 283.795 kb on - strand, within RR42_RS01255 at 283.816 kb on - strand, within RR42_RS01255 at 283.902 kb on + strand, within RR42_RS01255 at 284.075 kb on - strand, within RR42_RS01255 at 284.076 kb on + strand, within RR42_RS01255 at 284.076 kb on + strand, within RR42_RS01255 at 284.077 kb on - strand, within RR42_RS01255 at 284.400 kb on + strand, within RR42_RS01255 at 284.400 kb on + strand, within RR42_RS01255 at 284.652 kb on - strand, within RR42_RS01260 at 284.752 kb on - strand, within RR42_RS01260 at 284.752 kb on - strand, within RR42_RS01260 at 285.057 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.234 kb on - strand, within RR42_RS01260 at 285.481 kb on - strand at 285.481 kb on - strand at 285.481 kb on - strand at 285.537 kb on - strand at 285.537 kb on - strand at 285.551 kb on - strand at 285.689 kb on - strand at 285.760 kb on + strand at 285.766 kb on + strand at 285.787 kb on + strand at 285.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 283,523 + +0.6 283,523 + -0.6 283,617 + +0.2 283,617 + -2.5 283,669 - -1.3 283,672 - -0.9 283,785 + RR42_RS01255 0.19 -0.8 283,785 + RR42_RS01255 0.19 -1.2 283,795 - RR42_RS01255 0.20 -1.2 283,795 - RR42_RS01255 0.20 -1.3 283,816 - RR42_RS01255 0.23 -0.0 283,902 + RR42_RS01255 0.32 -0.8 284,075 - RR42_RS01255 0.52 -0.9 284,076 + RR42_RS01255 0.52 -2.2 284,076 + RR42_RS01255 0.52 -1.2 284,077 - RR42_RS01255 0.52 -2.5 284,400 + RR42_RS01255 0.88 -2.6 284,400 + RR42_RS01255 0.88 -0.7 284,652 - RR42_RS01260 0.16 -2.9 284,752 - RR42_RS01260 0.27 -2.8 284,752 - RR42_RS01260 0.27 -2.5 285,057 - RR42_RS01260 0.59 -1.6 285,198 - RR42_RS01260 0.74 -2.7 285,198 - RR42_RS01260 0.74 -2.0 285,198 - RR42_RS01260 0.74 -4.3 285,234 - RR42_RS01260 0.78 -2.7 285,481 - -0.3 285,481 - -0.3 285,481 - -0.4 285,537 - -2.0 285,537 - -0.5 285,551 - -0.2 285,689 - -0.3 285,760 + -1.3 285,766 + -0.0 285,787 + -0.4 285,787 + -0.7
Or see this region's nucleotide sequence