Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00695 and RR42_RS00700 overlap by 4 nucleotides RR42_RS00700 and RR42_RS00705 are separated by 57 nucleotides RR42_RS00705 and RR42_RS00710 are separated by 28 nucleotides
RR42_RS00695: RR42_RS00695 - biotin--[acetyl-CoA-carboxylase] synthetase, at 176,821 to 177,705
_RS00695
RR42_RS00700: RR42_RS00700 - pantothenate kinase, at 177,702 to 178,544
_RS00700
RR42_RS00705: RR42_RS00705 - hypothetical protein, at 178,602 to 179,330
_RS00705
RR42_RS00710: RR42_RS00710 - ADP-heptose synthase, at 179,359 to 179,847
_RS00710
Position (kb) 177
178
179 Strain fitness (log2 ratio) -2
-1
0
1 at 176.754 kb on + strand at 176.755 kb on - strand at 176.755 kb on - strand at 176.763 kb on + strand at 176.763 kb on + strand at 178.567 kb on + strand at 178.567 kb on + strand at 178.568 kb on - strand at 178.621 kb on + strand at 179.069 kb on + strand, within RR42_RS00705 at 179.070 kb on - strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.081 kb on - strand, within RR42_RS00705 at 179.081 kb on - strand, within RR42_RS00705 at 179.081 kb on - strand, within RR42_RS00705 at 179.112 kb on + strand, within RR42_RS00705 at 179.112 kb on + strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.114 kb on + strand, within RR42_RS00705 at 179.115 kb on - strand, within RR42_RS00705 at 179.115 kb on - strand, within RR42_RS00705
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 176,754 + +0.7 176,755 - +0.7 176,755 - -1.4 176,763 + -0.0 176,763 + +1.3 178,567 + +0.1 178,567 + +0.2 178,568 - +0.7 178,621 + +0.7 179,069 + RR42_RS00705 0.64 -1.0 179,070 - RR42_RS00705 0.64 +1.2 179,080 + RR42_RS00705 0.66 +0.7 179,080 + RR42_RS00705 0.66 +0.8 179,080 + RR42_RS00705 0.66 +0.4 179,080 + RR42_RS00705 0.66 -0.4 179,081 - RR42_RS00705 0.66 -0.0 179,081 - RR42_RS00705 0.66 +0.8 179,081 - RR42_RS00705 0.66 +0.8 179,112 + RR42_RS00705 0.70 +0.4 179,112 + RR42_RS00705 0.70 +0.6 179,113 - RR42_RS00705 0.70 +0.7 179,113 - RR42_RS00705 0.70 -1.9 179,113 - RR42_RS00705 0.70 -1.6 179,113 - RR42_RS00705 0.70 +1.0 179,113 - RR42_RS00705 0.70 +0.2 179,114 + RR42_RS00705 0.70 +1.1 179,115 - RR42_RS00705 0.70 +0.6 179,115 - RR42_RS00705 0.70 +0.5
Or see this region's nucleotide sequence