Experiment: R2A with nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00405 and RR42_RS00410 are separated by 121 nucleotides RR42_RS00410 and RR42_RS00415 are separated by 339 nucleotides
RR42_RS00405: RR42_RS00405 - glutamate carboxypeptidase, at 108,369 to 109,688
_RS00405
RR42_RS00410: RR42_RS00410 - transcriptional regulator, at 109,810 to 110,286
_RS00410
RR42_RS00415: RR42_RS00415 - ABC transporter permease, at 110,626 to 111,465
_RS00415
Position (kb) 109
110
111 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 108.907 kb on + strand, within RR42_RS00405 at 108.907 kb on + strand, within RR42_RS00405 at 108.908 kb on - strand, within RR42_RS00405 at 108.908 kb on - strand, within RR42_RS00405 at 108.908 kb on - strand, within RR42_RS00405 at 109.142 kb on - strand, within RR42_RS00405 at 109.178 kb on + strand, within RR42_RS00405 at 109.179 kb on - strand, within RR42_RS00405 at 109.179 kb on - strand, within RR42_RS00405 at 109.179 kb on - strand, within RR42_RS00405 at 109.282 kb on + strand, within RR42_RS00405 at 109.283 kb on - strand, within RR42_RS00405 at 109.289 kb on + strand, within RR42_RS00405 at 109.290 kb on - strand, within RR42_RS00405 at 109.295 kb on + strand, within RR42_RS00405 at 109.387 kb on + strand, within RR42_RS00405 at 109.388 kb on - strand, within RR42_RS00405 at 109.558 kb on + strand at 109.558 kb on + strand at 109.559 kb on - strand at 109.559 kb on - strand at 109.674 kb on - strand at 109.674 kb on - strand at 109.736 kb on + strand at 109.736 kb on + strand at 109.736 kb on + strand at 109.737 kb on - strand at 109.737 kb on - strand at 109.737 kb on - strand at 109.921 kb on + strand, within RR42_RS00410 at 109.921 kb on + strand, within RR42_RS00410 at 109.922 kb on - strand, within RR42_RS00410 at 110.105 kb on + strand, within RR42_RS00410 at 110.106 kb on - strand, within RR42_RS00410 at 110.200 kb on - strand, within RR42_RS00410 at 110.200 kb on - strand, within RR42_RS00410 at 110.286 kb on + strand at 110.446 kb on + strand at 110.609 kb on + strand at 110.610 kb on - strand at 110.665 kb on + strand at 110.666 kb on - strand at 110.666 kb on - strand at 110.666 kb on - strand at 110.818 kb on - strand, within RR42_RS00415 at 110.818 kb on - strand, within RR42_RS00415 at 111.227 kb on - strand, within RR42_RS00415 at 111.227 kb on - strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 150 mM remove 108,907 + RR42_RS00405 0.41 -2.1 108,907 + RR42_RS00405 0.41 +0.7 108,908 - RR42_RS00405 0.41 -0.5 108,908 - RR42_RS00405 0.41 -0.1 108,908 - RR42_RS00405 0.41 -0.0 109,142 - RR42_RS00405 0.59 +0.2 109,178 + RR42_RS00405 0.61 +0.3 109,179 - RR42_RS00405 0.61 -0.7 109,179 - RR42_RS00405 0.61 -0.4 109,179 - RR42_RS00405 0.61 -3.4 109,282 + RR42_RS00405 0.69 +0.3 109,283 - RR42_RS00405 0.69 +0.7 109,289 + RR42_RS00405 0.70 -0.4 109,290 - RR42_RS00405 0.70 +1.0 109,295 + RR42_RS00405 0.70 -0.5 109,387 + RR42_RS00405 0.77 +0.4 109,388 - RR42_RS00405 0.77 +0.5 109,558 + +0.9 109,558 + -0.1 109,559 - +0.6 109,559 - +0.8 109,674 - +0.8 109,674 - -0.1 109,736 + -0.6 109,736 + +0.3 109,736 + +0.4 109,737 - -0.1 109,737 - +0.7 109,737 - -0.1 109,921 + RR42_RS00410 0.23 +1.2 109,921 + RR42_RS00410 0.23 +0.2 109,922 - RR42_RS00410 0.23 -0.0 110,105 + RR42_RS00410 0.62 -0.2 110,106 - RR42_RS00410 0.62 -0.3 110,200 - RR42_RS00410 0.82 +0.3 110,200 - RR42_RS00410 0.82 -1.0 110,286 + -0.0 110,446 + +0.5 110,609 + +0.7 110,610 - -0.2 110,665 + +0.2 110,666 - -0.0 110,666 - -0.1 110,666 - +0.5 110,818 - RR42_RS00415 0.23 -0.3 110,818 - RR42_RS00415 0.23 -2.8 111,227 - RR42_RS00415 0.72 +2.1 111,227 - RR42_RS00415 0.72 -0.0 111,262 + RR42_RS00415 0.76 +0.4 111,262 + RR42_RS00415 0.76 +0.6 111,262 + RR42_RS00415 0.76 +0.1 111,263 - RR42_RS00415 0.76 -0.0 111,263 - RR42_RS00415 0.76 -0.3 111,263 - RR42_RS00415 0.76 +1.0
Or see this region's nucleotide sequence