Experiment: H2 N-free with 10mM L-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08720 and MMP_RS09070 are separated by 89 nucleotides MMP_RS09070 and MMP_RS08725 are separated by 15 nucleotides MMP_RS08725 and MMP_RS08730 are separated by 41 nucleotides
MMP_RS08720: MMP_RS08720 - 4Fe-4S binding protein, at 1,632,705 to 1,633,892
_RS08720
MMP_RS09070: MMP_RS09070 - F420 non-reducing hydrogenase subunit, at 1,633,982 to 1,634,116
_RS09070
MMP_RS08725: MMP_RS08725 - Ni/Fe hydrogenase subunit alpha, at 1,634,132 to 1,635,388
_RS08725
MMP_RS08730: MMP_RS08730 - F420-non-reducing hydrogenase subunit, at 1,635,430 to 1,636,296
_RS08730
Position (kb)
1634
1635
1636 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1633.411 kb on + strand, within MMP_RS08720 at 1633.412 kb on - strand, within MMP_RS08720 at 1633.668 kb on - strand, within MMP_RS08720 at 1633.746 kb on - strand, within MMP_RS08720 at 1634.354 kb on - strand, within MMP_RS08725 at 1634.721 kb on - strand, within MMP_RS08725 at 1635.324 kb on + strand at 1635.424 kb on + strand at 1635.892 kb on + strand at 1635.999 kb on + strand at 1636.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Alanine remove 1,633,411 + MMP_RS08720 0.59 -3.1 1,633,412 - MMP_RS08720 0.60 -2.7 1,633,668 - MMP_RS08720 0.81 -2.8 1,633,746 - MMP_RS08720 0.88 -2.5 1,634,354 - MMP_RS08725 0.18 -0.8 1,634,721 - MMP_RS08725 0.47 -3.4 1,635,324 + -3.4 1,635,424 + -3.9 1,635,892 + -3.8 1,635,999 + -0.6 1,636,112 + -3.4
Or see this region's nucleotide sequence