Experiment: H2 N-free with 10mM L-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04005 and MMP_RS04010 are separated by 360 nucleotides MMP_RS04010 and MMP_RS04015 are separated by 0 nucleotides MMP_RS04015 and MMP_RS04020 are separated by 11 nucleotides
MMP_RS04005: MMP_RS04005 - hypothetical protein, at 759,560 to 761,332
_RS04005
MMP_RS04010: MMP_RS04010 - ABC transporter substrate-binding protein, at 761,693 to 762,445
_RS04010
MMP_RS04015: MMP_RS04015 - hypothetical protein, at 762,446 to 762,937
_RS04015
MMP_RS04020: MMP_RS04020 - hypothetical protein, at 762,949 to 763,713
_RS04020
Position (kb) 761
762
763 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 760.710 kb on - strand, within MMP_RS04005 at 760.711 kb on + strand, within MMP_RS04005 at 760.714 kb on - strand, within MMP_RS04005 at 760.715 kb on + strand, within MMP_RS04005 at 760.721 kb on - strand, within MMP_RS04005 at 760.746 kb on - strand, within MMP_RS04005 at 760.757 kb on - strand, within MMP_RS04005 at 760.819 kb on + strand, within MMP_RS04005 at 760.819 kb on + strand, within MMP_RS04005 at 760.870 kb on + strand, within MMP_RS04005 at 760.889 kb on + strand, within MMP_RS04005 at 760.893 kb on + strand, within MMP_RS04005 at 760.992 kb on + strand, within MMP_RS04005 at 761.051 kb on - strand, within MMP_RS04005 at 761.147 kb on - strand, within MMP_RS04005 at 761.147 kb on - strand, within MMP_RS04005 at 761.148 kb on + strand, within MMP_RS04005 at 761.148 kb on + strand, within MMP_RS04005 at 761.148 kb on + strand, within MMP_RS04005 at 761.168 kb on - strand at 761.195 kb on + strand at 761.235 kb on + strand at 761.235 kb on + strand at 761.238 kb on + strand at 761.244 kb on - strand at 761.257 kb on + strand at 761.321 kb on - strand at 761.322 kb on + strand at 761.355 kb on - strand at 761.355 kb on - strand at 761.355 kb on - strand at 761.356 kb on + strand at 761.356 kb on + strand at 761.419 kb on - strand at 761.419 kb on - strand at 761.420 kb on + strand at 761.442 kb on - strand at 761.443 kb on + strand at 761.479 kb on - strand at 761.483 kb on - strand at 761.520 kb on - strand at 761.520 kb on - strand at 761.520 kb on - strand at 761.521 kb on + strand at 761.522 kb on - strand at 761.522 kb on - strand at 761.523 kb on + strand at 761.523 kb on + strand at 761.537 kb on + strand at 761.540 kb on + strand at 761.552 kb on - strand at 761.560 kb on - strand at 761.561 kb on + strand at 761.562 kb on - strand at 761.562 kb on - strand at 761.562 kb on - strand at 761.563 kb on + strand at 761.563 kb on + strand at 761.591 kb on - strand at 761.593 kb on - strand at 761.593 kb on - strand at 761.594 kb on + strand at 761.600 kb on + strand at 761.601 kb on - strand at 761.601 kb on - strand at 761.601 kb on - strand at 761.601 kb on - strand at 761.601 kb on - strand at 761.602 kb on + strand at 761.602 kb on + strand at 761.602 kb on + strand at 761.602 kb on + strand at 761.602 kb on + strand at 761.605 kb on - strand at 761.605 kb on - strand at 761.606 kb on + strand at 761.606 kb on + strand at 761.632 kb on - strand at 761.633 kb on + strand at 761.633 kb on + strand at 761.642 kb on - strand at 761.642 kb on - strand at 761.642 kb on - strand at 761.642 kb on - strand at 761.642 kb on - strand at 761.643 kb on + strand at 761.643 kb on + strand at 761.644 kb on - strand at 761.644 kb on - strand at 761.645 kb on + strand at 761.645 kb on + strand at 761.646 kb on - strand at 761.689 kb on - strand at 761.690 kb on + strand at 761.803 kb on - strand, within MMP_RS04010 at 761.803 kb on - strand, within MMP_RS04010 at 761.812 kb on + strand, within MMP_RS04010 at 761.826 kb on + strand, within MMP_RS04010 at 761.828 kb on + strand, within MMP_RS04010 at 761.860 kb on + strand, within MMP_RS04010 at 761.860 kb on + strand, within MMP_RS04010 at 761.865 kb on - strand, within MMP_RS04010 at 761.866 kb on + strand, within MMP_RS04010 at 761.868 kb on - strand, within MMP_RS04010 at 761.869 kb on + strand, within MMP_RS04010 at 761.871 kb on + strand, within MMP_RS04010 at 761.895 kb on - strand, within MMP_RS04010 at 761.896 kb on + strand, within MMP_RS04010 at 761.913 kb on - strand, within MMP_RS04010 at 761.913 kb on - strand, within MMP_RS04010 at 761.970 kb on - strand, within MMP_RS04010 at 761.975 kb on - strand, within MMP_RS04010 at 761.976 kb on + strand, within MMP_RS04010 at 762.034 kb on - strand, within MMP_RS04010 at 762.038 kb on - strand, within MMP_RS04010 at 762.038 kb on - strand, within MMP_RS04010 at 762.038 kb on - strand, within MMP_RS04010 at 762.048 kb on - strand, within MMP_RS04010 at 762.068 kb on + strand, within MMP_RS04010 at 762.216 kb on - strand, within MMP_RS04010 at 762.250 kb on - strand, within MMP_RS04010 at 762.251 kb on + strand, within MMP_RS04010 at 762.253 kb on - strand, within MMP_RS04010 at 762.254 kb on + strand, within MMP_RS04010 at 762.263 kb on - strand, within MMP_RS04010 at 762.267 kb on - strand, within MMP_RS04010 at 762.268 kb on + strand, within MMP_RS04010 at 762.278 kb on - strand, within MMP_RS04010 at 762.278 kb on - strand, within MMP_RS04010 at 762.278 kb on - strand, within MMP_RS04010 at 762.323 kb on - strand, within MMP_RS04010 at 762.323 kb on - strand, within MMP_RS04010 at 762.346 kb on - strand, within MMP_RS04010 at 762.411 kb on - strand at 762.415 kb on - strand at 762.420 kb on - strand at 762.421 kb on + strand at 762.437 kb on + strand at 762.437 kb on + strand at 762.447 kb on - strand at 762.447 kb on - strand at 762.447 kb on - strand at 762.448 kb on + strand at 762.751 kb on - strand, within MMP_RS04015 at 762.824 kb on + strand, within MMP_RS04015 at 762.824 kb on + strand, within MMP_RS04015 at 762.950 kb on - strand at 763.085 kb on - strand, within MMP_RS04020 at 763.184 kb on + strand, within MMP_RS04020 at 763.224 kb on + strand, within MMP_RS04020 at 763.276 kb on - strand, within MMP_RS04020 at 763.280 kb on + strand, within MMP_RS04020 at 763.282 kb on + strand, within MMP_RS04020 at 763.373 kb on + strand, within MMP_RS04020
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Alanine remove 760,710 - MMP_RS04005 0.65 -0.1 760,711 + MMP_RS04005 0.65 +0.6 760,714 - MMP_RS04005 0.65 -0.7 760,715 + MMP_RS04005 0.65 -0.3 760,721 - MMP_RS04005 0.65 -1.5 760,746 - MMP_RS04005 0.67 +0.5 760,757 - MMP_RS04005 0.68 -1.2 760,819 + MMP_RS04005 0.71 +0.6 760,819 + MMP_RS04005 0.71 -0.3 760,870 + MMP_RS04005 0.74 +1.0 760,889 + MMP_RS04005 0.75 +0.2 760,893 + MMP_RS04005 0.75 +1.3 760,992 + MMP_RS04005 0.81 -0.7 761,051 - MMP_RS04005 0.84 -0.3 761,147 - MMP_RS04005 0.90 +0.3 761,147 - MMP_RS04005 0.90 +0.6 761,148 + MMP_RS04005 0.90 +1.7 761,148 + MMP_RS04005 0.90 +0.4 761,148 + MMP_RS04005 0.90 +0.4 761,168 - -0.1 761,195 + -0.4 761,235 + -0.4 761,235 + +1.8 761,238 + +0.6 761,244 - -0.9 761,257 + +0.8 761,321 - +0.1 761,322 + +0.5 761,355 - +0.0 761,355 - +0.7 761,355 - -0.1 761,356 + +0.1 761,356 + -1.2 761,419 - +1.2 761,419 - -2.5 761,420 + +1.4 761,442 - +0.4 761,443 + -0.5 761,479 - -1.7 761,483 - +0.3 761,520 - +0.3 761,520 - +0.5 761,520 - +0.5 761,521 + -0.3 761,522 - +0.6 761,522 - +0.4 761,523 + -0.3 761,523 + +0.5 761,537 + -1.5 761,540 + -0.2 761,552 - +1.8 761,560 - -0.1 761,561 + -1.4 761,562 - -1.2 761,562 - +0.3 761,562 - -1.3 761,563 + -0.3 761,563 + +0.0 761,591 - +0.7 761,593 - -0.3 761,593 - -0.2 761,594 + -2.6 761,600 + -0.4 761,601 - +0.2 761,601 - +0.5 761,601 - -0.1 761,601 - -0.0 761,601 - -0.9 761,602 + -1.0 761,602 + +2.2 761,602 + +0.5 761,602 + -5.2 761,602 + -0.2 761,605 - -0.9 761,605 - +0.9 761,606 + +1.1 761,606 + +0.2 761,632 - +0.0 761,633 + +0.3 761,633 + +0.1 761,642 - +0.2 761,642 - -0.5 761,642 - +0.9 761,642 - -1.2 761,642 - +2.0 761,643 + -0.7 761,643 + -0.9 761,644 - +0.2 761,644 - -0.4 761,645 + -1.0 761,645 + +0.7 761,646 - -1.6 761,689 - +0.9 761,690 + +0.4 761,803 - MMP_RS04010 0.15 +0.1 761,803 - MMP_RS04010 0.15 -0.1 761,812 + MMP_RS04010 0.16 +0.6 761,826 + MMP_RS04010 0.18 -0.6 761,828 + MMP_RS04010 0.18 -0.3 761,860 + MMP_RS04010 0.22 -0.0 761,860 + MMP_RS04010 0.22 -2.3 761,865 - MMP_RS04010 0.23 +1.0 761,866 + MMP_RS04010 0.23 -0.0 761,868 - MMP_RS04010 0.23 +0.3 761,869 + MMP_RS04010 0.23 +0.3 761,871 + MMP_RS04010 0.24 -1.5 761,895 - MMP_RS04010 0.27 -0.3 761,896 + MMP_RS04010 0.27 +0.3 761,913 - MMP_RS04010 0.29 -0.5 761,913 - MMP_RS04010 0.29 -0.6 761,970 - MMP_RS04010 0.37 +1.5 761,975 - MMP_RS04010 0.37 +0.6 761,976 + MMP_RS04010 0.38 +0.7 762,034 - MMP_RS04010 0.45 -0.8 762,038 - MMP_RS04010 0.46 -0.4 762,038 - MMP_RS04010 0.46 +0.3 762,038 - MMP_RS04010 0.46 +1.0 762,048 - MMP_RS04010 0.47 +0.2 762,068 + MMP_RS04010 0.50 +0.2 762,216 - MMP_RS04010 0.69 -1.0 762,250 - MMP_RS04010 0.74 -0.0 762,251 + MMP_RS04010 0.74 +0.4 762,253 - MMP_RS04010 0.74 -2.4 762,254 + MMP_RS04010 0.75 -0.1 762,263 - MMP_RS04010 0.76 -1.0 762,267 - MMP_RS04010 0.76 -0.7 762,268 + MMP_RS04010 0.76 +0.5 762,278 - MMP_RS04010 0.78 +0.1 762,278 - MMP_RS04010 0.78 -0.4 762,278 - MMP_RS04010 0.78 -0.0 762,323 - MMP_RS04010 0.84 -1.5 762,323 - MMP_RS04010 0.84 -0.9 762,346 - MMP_RS04010 0.87 -1.4 762,411 - +0.1 762,415 - +0.2 762,420 - +0.7 762,421 + -1.1 762,437 + -1.4 762,437 + +2.3 762,447 - +0.2 762,447 - +0.5 762,447 - -1.2 762,448 + +1.4 762,751 - MMP_RS04015 0.62 +1.1 762,824 + MMP_RS04015 0.77 -1.4 762,824 + MMP_RS04015 0.77 +0.1 762,950 - +0.3 763,085 - MMP_RS04020 0.18 +1.2 763,184 + MMP_RS04020 0.31 +0.7 763,224 + MMP_RS04020 0.36 +1.8 763,276 - MMP_RS04020 0.43 +0.6 763,280 + MMP_RS04020 0.43 +0.8 763,282 + MMP_RS04020 0.44 -0.2 763,373 + MMP_RS04020 0.55 +0.0
Or see this region's nucleotide sequence