Experiment: H2 N-free with 10mM L-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00355 and MMP_RS00360 are separated by 36 nucleotides MMP_RS00360 and MMP_RS00365 are separated by 94 nucleotides MMP_RS00365 and MMP_RS00370 are separated by 391 nucleotides MMP_RS00370 and MMP_RS00375 are separated by 26 nucleotides
MMP_RS00355: MMP_RS00355 - translation initiation factor IF-6, at 74,409 to 75,092
_RS00355
MMP_RS00360: MMP_RS00360 - 50S ribosomal protein L31e, at 75,129 to 75,380
_RS00360
MMP_RS00365: MMP_RS00365 - acetylglutamate kinase, at 75,475 to 76,359
_RS00365
MMP_RS00370: MMP_RS00370 - P-II family nitrogen regulator, at 76,751 to 77,089
_RS00370
MMP_RS00375: MMP_RS00375 - ammonium transporter, at 77,116 to 78,342
_RS00375
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 74.688 kb on - strand at 74.763 kb on - strand at 75.135 kb on - strand at 75.783 kb on + strand at 75.962 kb on - strand at 75.968 kb on - strand at 76.097 kb on - strand at 76.350 kb on + strand at 76.402 kb on + strand at 76.413 kb on + strand at 76.418 kb on - strand at 76.469 kb on - strand at 76.470 kb on + strand at 76.475 kb on - strand at 76.475 kb on - strand at 76.479 kb on - strand at 76.496 kb on - strand at 76.497 kb on + strand at 76.497 kb on + strand at 76.551 kb on - strand at 76.552 kb on + strand at 76.571 kb on + strand at 76.634 kb on - strand at 76.635 kb on + strand at 76.636 kb on - strand at 76.636 kb on - strand at 76.636 kb on - strand at 76.637 kb on + strand at 76.768 kb on - strand at 77.037 kb on - strand at 77.076 kb on - strand at 77.193 kb on + strand at 77.201 kb on - strand at 77.204 kb on - strand at 77.205 kb on + strand at 77.292 kb on + strand, within MMP_RS00375
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Alanine remove 74,688 - -2.9 74,763 - -2.3 75,135 - -1.8 75,783 + -3.3 75,962 - -2.2 75,968 - -3.4 76,097 - -2.3 76,350 + -2.8 76,402 + -1.2 76,413 + +1.8 76,418 - -0.3 76,469 - -0.7 76,470 + -0.4 76,475 - +0.1 76,475 - +0.8 76,479 - +0.2 76,496 - -0.7 76,497 + -0.8 76,497 + +0.8 76,551 - -0.1 76,552 + +2.2 76,571 + +0.1 76,634 - +0.5 76,635 + +0.0 76,636 - -2.0 76,636 - -0.1 76,636 - +0.1 76,637 + -2.2 76,768 - -1.8 77,037 - -3.9 77,076 - -4.7 77,193 + -1.9 77,201 - -3.0 77,204 - -0.2 77,205 + -0.9 77,292 + MMP_RS00375 0.14 -0.8
Or see this region's nucleotide sequence