Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32650

Experiment: R2A with Cisplatin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32640 and RR42_RS32645 are separated by 32 nucleotidesRR42_RS32645 and RR42_RS32650 are separated by 8 nucleotidesRR42_RS32650 and RR42_RS32655 are separated by 144 nucleotides RR42_RS32640: RR42_RS32640 - 4-oxalocrotonate decarboxylase, at 2,719,782 to 2,720,570 _RS32640 RR42_RS32645: RR42_RS32645 - 2-keto-4-pentenoate hydratase, at 2,720,603 to 2,721,385 _RS32645 RR42_RS32650: RR42_RS32650 - betaine-aldehyde dehydrogenase, at 2,721,394 to 2,722,848 _RS32650 RR42_RS32655: RR42_RS32655 - catechol 2,3-dioxygenase, at 2,722,993 to 2,723,925 _RS32655 Position (kb) 2721 2722 2723Strain fitness (log2 ratio) -2 -1 0 1at 2720.589 kb on + strandat 2721.265 kb on + strand, within RR42_RS32645at 2721.271 kb on - strand, within RR42_RS32645at 2721.371 kb on - strandat 2721.428 kb on + strandat 2721.428 kb on + strandat 2721.663 kb on + strand, within RR42_RS32650at 2721.830 kb on + strand, within RR42_RS32650at 2721.972 kb on - strand, within RR42_RS32650at 2721.972 kb on - strand, within RR42_RS32650at 2722.111 kb on + strand, within RR42_RS32650at 2722.149 kb on + strand, within RR42_RS32650at 2722.244 kb on + strand, within RR42_RS32650at 2722.440 kb on - strand, within RR42_RS32650at 2722.440 kb on - strand, within RR42_RS32650at 2722.648 kb on + strand, within RR42_RS32650at 2722.648 kb on + strand, within RR42_RS32650at 2723.002 kb on + strandat 2723.003 kb on - strandat 2723.153 kb on + strand, within RR42_RS32655at 2723.172 kb on + strand, within RR42_RS32655at 2723.723 kb on + strand, within RR42_RS32655at 2723.724 kb on - strand, within RR42_RS32655at 2723.796 kb on - strand, within RR42_RS32655

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml
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2,720,589 + +0.7
2,721,265 + RR42_RS32645 0.85 +0.7
2,721,271 - RR42_RS32645 0.85 -1.4
2,721,371 - +0.1
2,721,428 + -1.3
2,721,428 + -1.8
2,721,663 + RR42_RS32650 0.18 -0.8
2,721,830 + RR42_RS32650 0.30 +0.7
2,721,972 - RR42_RS32650 0.40 +0.6
2,721,972 - RR42_RS32650 0.40 -0.7
2,722,111 + RR42_RS32650 0.49 +0.9
2,722,149 + RR42_RS32650 0.52 -0.4
2,722,244 + RR42_RS32650 0.58 -0.2
2,722,440 - RR42_RS32650 0.72 +0.3
2,722,440 - RR42_RS32650 0.72 +0.3
2,722,648 + RR42_RS32650 0.86 -0.0
2,722,648 + RR42_RS32650 0.86 -0.4
2,723,002 + -1.8
2,723,003 - -0.2
2,723,153 + RR42_RS32655 0.17 -0.2
2,723,172 + RR42_RS32655 0.19 +1.2
2,723,723 + RR42_RS32655 0.78 -0.2
2,723,724 - RR42_RS32655 0.78 +0.2
2,723,796 - RR42_RS32655 0.86 +1.1

Or see this region's nucleotide sequence