Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17660 and RR42_RS17665 are separated by 28 nucleotides RR42_RS17665 and RR42_RS17670 are separated by 85 nucleotides RR42_RS17670 and RR42_RS17675 are separated by 12 nucleotides
RR42_RS17660: RR42_RS17660 - 3-oxoacyl-ACP synthase, at 3,822,184 to 3,822,924
_RS17660
RR42_RS17665: RR42_RS17665 - chorismate lyase, at 3,822,953 to 3,823,594
_RS17665
RR42_RS17670: RR42_RS17670 - esterase, at 3,823,680 to 3,824,267
_RS17670
RR42_RS17675: RR42_RS17675 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 3,824,280 to 3,825,677
_RS17675
Position (kb)
3823
3824
3825 Strain fitness (log2 ratio)
-2
-1
0
1 at 3822.749 kb on + strand, within RR42_RS17660 at 3822.749 kb on + strand, within RR42_RS17660 at 3822.749 kb on + strand, within RR42_RS17660 at 3822.749 kb on + strand, within RR42_RS17660 at 3822.749 kb on + strand, within RR42_RS17660 at 3822.988 kb on + strand at 3822.988 kb on + strand at 3822.989 kb on - strand at 3822.989 kb on - strand at 3823.056 kb on - strand, within RR42_RS17665 at 3823.154 kb on + strand, within RR42_RS17665 at 3823.318 kb on + strand, within RR42_RS17665 at 3823.550 kb on + strand at 3823.550 kb on + strand at 3823.550 kb on + strand at 3823.726 kb on + strand at 3823.726 kb on + strand at 3823.727 kb on - strand at 3823.727 kb on - strand at 3823.849 kb on + strand, within RR42_RS17670 at 3823.849 kb on + strand, within RR42_RS17670 at 3823.850 kb on - strand, within RR42_RS17670 at 3824.044 kb on + strand, within RR42_RS17670 at 3824.044 kb on + strand, within RR42_RS17670 at 3824.044 kb on + strand, within RR42_RS17670 at 3824.045 kb on - strand, within RR42_RS17670 at 3824.045 kb on - strand, within RR42_RS17670 at 3824.170 kb on + strand, within RR42_RS17670 at 3824.170 kb on + strand, within RR42_RS17670 at 3824.170 kb on + strand, within RR42_RS17670 at 3824.171 kb on - strand, within RR42_RS17670 at 3824.171 kb on - strand, within RR42_RS17670 at 3824.174 kb on - strand, within RR42_RS17670 at 3824.174 kb on - strand, within RR42_RS17670 at 3824.257 kb on + strand at 3824.257 kb on + strand at 3824.257 kb on + strand at 3824.258 kb on - strand at 3824.258 kb on - strand at 3824.258 kb on - strand at 3824.258 kb on - strand at 3824.258 kb on - strand at 3824.259 kb on + strand at 3824.259 kb on + strand at 3824.259 kb on + strand at 3824.259 kb on + strand at 3824.259 kb on + strand at 3824.259 kb on + strand at 3824.260 kb on - strand at 3824.677 kb on + strand, within RR42_RS17675 at 3824.677 kb on + strand, within RR42_RS17675 at 3824.677 kb on + strand, within RR42_RS17675 at 3824.677 kb on + strand, within RR42_RS17675 at 3824.677 kb on + strand, within RR42_RS17675 at 3824.677 kb on + strand, within RR42_RS17675 at 3824.678 kb on - strand, within RR42_RS17675 at 3824.819 kb on + strand, within RR42_RS17675 at 3824.820 kb on - strand at 3824.820 kb on - strand, within RR42_RS17675 at 3825.115 kb on + strand, within RR42_RS17675 at 3825.116 kb on - strand, within RR42_RS17675 at 3825.116 kb on - strand, within RR42_RS17675 at 3825.116 kb on - strand, within RR42_RS17675 at 3825.116 kb on - strand, within RR42_RS17675 at 3825.188 kb on + strand, within RR42_RS17675 at 3825.194 kb on - strand, within RR42_RS17675 at 3825.194 kb on - strand, within RR42_RS17675 at 3825.194 kb on - strand, within RR42_RS17675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,822,749 + RR42_RS17660 0.76 +0.3 3,822,749 + RR42_RS17660 0.76 -0.1 3,822,749 + RR42_RS17660 0.76 +0.3 3,822,749 + RR42_RS17660 0.76 +0.6 3,822,749 + RR42_RS17660 0.76 +0.8 3,822,988 + +0.1 3,822,988 + -0.9 3,822,989 - +0.5 3,822,989 - -0.1 3,823,056 - RR42_RS17665 0.16 +0.5 3,823,154 + RR42_RS17665 0.31 -0.4 3,823,318 + RR42_RS17665 0.57 +0.3 3,823,550 + +0.9 3,823,550 + +1.1 3,823,550 + +0.7 3,823,726 + +0.1 3,823,726 + +0.2 3,823,727 - -0.2 3,823,727 - +0.2 3,823,849 + RR42_RS17670 0.29 +0.5 3,823,849 + RR42_RS17670 0.29 +0.4 3,823,850 - RR42_RS17670 0.29 +0.4 3,824,044 + RR42_RS17670 0.62 -0.1 3,824,044 + RR42_RS17670 0.62 +1.0 3,824,044 + RR42_RS17670 0.62 +0.1 3,824,045 - RR42_RS17670 0.62 +0.4 3,824,045 - RR42_RS17670 0.62 +0.8 3,824,170 + RR42_RS17670 0.83 -0.2 3,824,170 + RR42_RS17670 0.83 +0.1 3,824,170 + RR42_RS17670 0.83 +0.0 3,824,171 - RR42_RS17670 0.84 +0.5 3,824,171 - RR42_RS17670 0.84 -0.1 3,824,174 - RR42_RS17670 0.84 +0.5 3,824,174 - RR42_RS17670 0.84 -0.5 3,824,257 + -0.9 3,824,257 + +0.4 3,824,257 + -0.9 3,824,258 - -0.6 3,824,258 - +0.5 3,824,258 - -1.1 3,824,258 - -0.1 3,824,258 - +0.2 3,824,259 + -1.9 3,824,259 + -0.2 3,824,259 + -0.3 3,824,259 + +0.4 3,824,259 + +1.4 3,824,259 + -1.2 3,824,260 - -0.1 3,824,677 + RR42_RS17675 0.28 -0.2 3,824,677 + RR42_RS17675 0.28 +0.3 3,824,677 + RR42_RS17675 0.28 +0.3 3,824,677 + RR42_RS17675 0.28 -0.5 3,824,677 + RR42_RS17675 0.28 +0.3 3,824,677 + RR42_RS17675 0.28 -0.0 3,824,678 - RR42_RS17675 0.28 -0.7 3,824,819 + RR42_RS17675 0.39 +0.4 3,824,820 - -0.7 3,824,820 - RR42_RS17675 0.39 +0.1 3,825,115 + RR42_RS17675 0.60 -0.1 3,825,116 - RR42_RS17675 0.60 +1.0 3,825,116 - RR42_RS17675 0.60 +0.4 3,825,116 - RR42_RS17675 0.60 +0.2 3,825,116 - RR42_RS17675 0.60 -1.1 3,825,188 + RR42_RS17675 0.65 +0.1 3,825,194 - RR42_RS17675 0.65 -0.0 3,825,194 - RR42_RS17675 0.65 -0.1 3,825,194 - RR42_RS17675 0.65 +0.4
Or see this region's nucleotide sequence