Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17250 and RR42_RS17255 are separated by 13 nucleotides RR42_RS17255 and RR42_RS17260 are separated by 53 nucleotides RR42_RS17260 and RR42_RS17265 are separated by 9 nucleotides
RR42_RS17250: RR42_RS17250 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,734,113 to 3,734,934
_RS17250
RR42_RS17255: RR42_RS17255 - ABC transporter permease, at 3,734,948 to 3,735,646
_RS17255
RR42_RS17260: RR42_RS17260 - ABC transporter ATP-binding protein, at 3,735,700 to 3,736,374
_RS17260
RR42_RS17265: RR42_RS17265 - aminodeoxychorismate synthase, at 3,736,384 to 3,738,147
_RS17265
Position (kb)
3734
3735
3736 Strain fitness (log2 ratio)
-1
0
1
2 at 3733.993 kb on + strand at 3735.089 kb on + strand, within RR42_RS17255 at 3735.090 kb on - strand, within RR42_RS17255 at 3735.090 kb on - strand, within RR42_RS17255 at 3735.188 kb on + strand, within RR42_RS17255 at 3735.238 kb on + strand, within RR42_RS17255 at 3735.485 kb on - strand, within RR42_RS17255 at 3735.556 kb on + strand, within RR42_RS17255 at 3735.557 kb on - strand, within RR42_RS17255 at 3735.741 kb on - strand at 3735.763 kb on - strand at 3735.961 kb on + strand, within RR42_RS17260 at 3735.961 kb on + strand, within RR42_RS17260 at 3736.015 kb on + strand, within RR42_RS17260 at 3736.015 kb on + strand, within RR42_RS17260 at 3736.411 kb on + strand at 3736.411 kb on + strand at 3736.411 kb on + strand at 3736.412 kb on - strand at 3736.441 kb on + strand at 3736.442 kb on - strand at 3736.442 kb on - strand at 3736.505 kb on + strand at 3736.506 kb on - strand at 3736.522 kb on + strand at 3736.522 kb on + strand at 3736.522 kb on + strand at 3736.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,733,993 + +0.4 3,735,089 + RR42_RS17255 0.20 -0.5 3,735,090 - RR42_RS17255 0.20 +0.4 3,735,090 - RR42_RS17255 0.20 +0.6 3,735,188 + RR42_RS17255 0.34 +1.5 3,735,238 + RR42_RS17255 0.41 -0.5 3,735,485 - RR42_RS17255 0.77 +0.6 3,735,556 + RR42_RS17255 0.87 -0.2 3,735,557 - RR42_RS17255 0.87 +0.7 3,735,741 - +0.2 3,735,763 - -0.3 3,735,961 + RR42_RS17260 0.39 +1.2 3,735,961 + RR42_RS17260 0.39 +0.2 3,736,015 + RR42_RS17260 0.47 -0.2 3,736,015 + RR42_RS17260 0.47 +1.6 3,736,411 + +0.6 3,736,411 + +2.0 3,736,411 + +0.9 3,736,412 - +0.4 3,736,441 + +0.2 3,736,442 - -0.4 3,736,442 - -1.1 3,736,505 + +1.0 3,736,506 - -0.7 3,736,522 + +0.2 3,736,522 + -0.0 3,736,522 + -0.1 3,736,523 - -1.0
Or see this region's nucleotide sequence