Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17085 and RR42_RS17090 are separated by 100 nucleotides RR42_RS17090 and RR42_RS17095 are separated by 39 nucleotides RR42_RS17095 and RR42_RS17100 are separated by 14 nucleotides
RR42_RS17085: RR42_RS17085 - luciferase, at 3,702,246 to 3,703,268
_RS17085
RR42_RS17090: RR42_RS17090 - aminotransferase, at 3,703,369 to 3,703,869
_RS17090
RR42_RS17095: RR42_RS17095 - bleomycin resistance protein, at 3,703,909 to 3,704,751
_RS17095
RR42_RS17100: RR42_RS17100 - membrane protein, at 3,704,766 to 3,705,779
_RS17100
Position (kb)
3703
3704
3705 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3702.916 kb on - strand, within RR42_RS17085 at 3702.916 kb on - strand, within RR42_RS17085 at 3702.917 kb on + strand, within RR42_RS17085 at 3702.918 kb on - strand, within RR42_RS17085 at 3702.918 kb on - strand, within RR42_RS17085 at 3702.918 kb on - strand, within RR42_RS17085 at 3702.918 kb on - strand, within RR42_RS17085 at 3703.011 kb on + strand, within RR42_RS17085 at 3703.012 kb on - strand, within RR42_RS17085 at 3703.227 kb on + strand at 3703.228 kb on - strand at 3703.309 kb on - strand at 3703.345 kb on + strand at 3703.867 kb on + strand at 3703.867 kb on + strand at 3703.868 kb on - strand at 3703.868 kb on - strand at 3703.868 kb on - strand at 3703.903 kb on - strand at 3704.211 kb on - strand, within RR42_RS17095 at 3704.262 kb on + strand, within RR42_RS17095 at 3704.262 kb on + strand, within RR42_RS17095 at 3704.262 kb on + strand, within RR42_RS17095 at 3704.262 kb on + strand, within RR42_RS17095 at 3704.262 kb on + strand, within RR42_RS17095 at 3704.263 kb on - strand, within RR42_RS17095 at 3704.292 kb on + strand, within RR42_RS17095 at 3704.493 kb on + strand, within RR42_RS17095 at 3704.494 kb on - strand, within RR42_RS17095 at 3704.552 kb on + strand, within RR42_RS17095 at 3704.555 kb on + strand, within RR42_RS17095 at 3704.555 kb on + strand, within RR42_RS17095 at 3704.601 kb on - strand, within RR42_RS17095 at 3704.734 kb on + strand at 3704.734 kb on + strand at 3704.992 kb on + strand, within RR42_RS17100 at 3704.993 kb on - strand, within RR42_RS17100 at 3704.993 kb on - strand, within RR42_RS17100 at 3704.993 kb on - strand, within RR42_RS17100 at 3705.121 kb on + strand, within RR42_RS17100 at 3705.135 kb on + strand, within RR42_RS17100 at 3705.136 kb on - strand, within RR42_RS17100 at 3705.136 kb on - strand, within RR42_RS17100 at 3705.187 kb on + strand, within RR42_RS17100 at 3705.187 kb on + strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.249 kb on - strand, within RR42_RS17100 at 3705.274 kb on + strand, within RR42_RS17100 at 3705.407 kb on + strand, within RR42_RS17100 at 3705.408 kb on - strand, within RR42_RS17100 at 3705.408 kb on - strand, within RR42_RS17100 at 3705.412 kb on + strand, within RR42_RS17100 at 3705.427 kb on + strand, within RR42_RS17100 at 3705.428 kb on - strand, within RR42_RS17100 at 3705.428 kb on - strand, within RR42_RS17100
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,702,916 - RR42_RS17085 0.65 -0.9 3,702,916 - RR42_RS17085 0.65 -1.4 3,702,917 + RR42_RS17085 0.66 +0.3 3,702,918 - RR42_RS17085 0.66 +0.5 3,702,918 - RR42_RS17085 0.66 +0.7 3,702,918 - RR42_RS17085 0.66 +0.4 3,702,918 - RR42_RS17085 0.66 +0.9 3,703,011 + RR42_RS17085 0.75 +0.4 3,703,012 - RR42_RS17085 0.75 -1.1 3,703,227 + +0.2 3,703,228 - -0.8 3,703,309 - +0.0 3,703,345 + +1.2 3,703,867 + +2.7 3,703,867 + +0.2 3,703,868 - -1.5 3,703,868 - +0.5 3,703,868 - -0.5 3,703,903 - -0.1 3,704,211 - RR42_RS17095 0.36 -0.5 3,704,262 + RR42_RS17095 0.42 -0.1 3,704,262 + RR42_RS17095 0.42 -1.1 3,704,262 + RR42_RS17095 0.42 -0.2 3,704,262 + RR42_RS17095 0.42 -0.1 3,704,262 + RR42_RS17095 0.42 -0.7 3,704,263 - RR42_RS17095 0.42 -2.3 3,704,292 + RR42_RS17095 0.45 +0.8 3,704,493 + RR42_RS17095 0.69 +0.3 3,704,494 - RR42_RS17095 0.69 -0.4 3,704,552 + RR42_RS17095 0.76 +0.3 3,704,555 + RR42_RS17095 0.77 +0.2 3,704,555 + RR42_RS17095 0.77 -0.1 3,704,601 - RR42_RS17095 0.82 +0.7 3,704,734 + +0.1 3,704,734 + +0.6 3,704,992 + RR42_RS17100 0.22 -0.1 3,704,993 - RR42_RS17100 0.22 -0.6 3,704,993 - RR42_RS17100 0.22 -0.1 3,704,993 - RR42_RS17100 0.22 -0.3 3,705,121 + RR42_RS17100 0.35 -0.6 3,705,135 + RR42_RS17100 0.36 +2.6 3,705,136 - RR42_RS17100 0.36 +0.0 3,705,136 - RR42_RS17100 0.36 +0.7 3,705,187 + RR42_RS17100 0.42 +0.1 3,705,187 + RR42_RS17100 0.42 -0.6 3,705,188 - RR42_RS17100 0.42 -1.4 3,705,188 - RR42_RS17100 0.42 +0.9 3,705,188 - RR42_RS17100 0.42 -0.1 3,705,188 - RR42_RS17100 0.42 +0.3 3,705,188 - RR42_RS17100 0.42 -0.3 3,705,249 - RR42_RS17100 0.48 +0.3 3,705,274 + RR42_RS17100 0.50 -0.6 3,705,407 + RR42_RS17100 0.63 -1.1 3,705,408 - RR42_RS17100 0.63 -0.4 3,705,408 - RR42_RS17100 0.63 +1.7 3,705,412 + RR42_RS17100 0.64 -0.7 3,705,427 + RR42_RS17100 0.65 +0.5 3,705,428 - RR42_RS17100 0.65 +0.1 3,705,428 - RR42_RS17100 0.65 +0.1
Or see this region's nucleotide sequence