Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15530 and RR42_RS15535 are separated by 74 nucleotides RR42_RS15535 and RR42_RS15540 are separated by 95 nucleotides RR42_RS15540 and RR42_RS15545 are separated by 64 nucleotides RR42_RS15545 and RR42_RS15550 are separated by 7 nucleotides
RR42_RS15530: RR42_RS15530 - membrane protein, at 3,355,263 to 3,356,195
_RS15530
RR42_RS15535: RR42_RS15535 - hypothetical protein, at 3,356,270 to 3,356,818
_RS15535
RR42_RS15540: RR42_RS15540 - hypothetical protein, at 3,356,914 to 3,358,347
_RS15540
RR42_RS15545: RR42_RS15545 - pilus assembly protein PilE, at 3,358,412 to 3,358,861
_RS15545
RR42_RS15550: RR42_RS15550 - pilus assembly protein, at 3,358,869 to 3,359,441
_RS15550
Position (kb)
3356
3357
3358
3359 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3355.970 kb on - strand, within RR42_RS15530 at 3355.970 kb on - strand, within RR42_RS15530 at 3356.042 kb on + strand, within RR42_RS15530 at 3356.043 kb on - strand, within RR42_RS15530 at 3356.043 kb on - strand, within RR42_RS15530 at 3356.165 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.189 kb on - strand at 3356.189 kb on - strand at 3356.189 kb on - strand at 3356.342 kb on + strand, within RR42_RS15535 at 3356.342 kb on + strand, within RR42_RS15535 at 3356.443 kb on - strand, within RR42_RS15535 at 3356.446 kb on + strand, within RR42_RS15535 at 3356.661 kb on - strand, within RR42_RS15535 at 3356.715 kb on - strand, within RR42_RS15535 at 3356.715 kb on - strand, within RR42_RS15535 at 3356.796 kb on + strand at 3356.796 kb on + strand at 3356.797 kb on - strand at 3356.797 kb on - strand at 3357.057 kb on - strand at 3357.249 kb on - strand, within RR42_RS15540 at 3357.350 kb on - strand, within RR42_RS15540 at 3357.577 kb on - strand, within RR42_RS15540 at 3357.601 kb on + strand, within RR42_RS15540 at 3357.602 kb on - strand, within RR42_RS15540 at 3357.691 kb on + strand, within RR42_RS15540 at 3357.691 kb on + strand, within RR42_RS15540 at 3357.692 kb on - strand, within RR42_RS15540 at 3357.835 kb on - strand, within RR42_RS15540 at 3357.858 kb on + strand, within RR42_RS15540 at 3357.859 kb on - strand, within RR42_RS15540 at 3357.952 kb on + strand, within RR42_RS15540 at 3357.953 kb on - strand, within RR42_RS15540 at 3357.953 kb on - strand, within RR42_RS15540 at 3358.057 kb on + strand, within RR42_RS15540 at 3358.124 kb on + strand, within RR42_RS15540 at 3358.124 kb on + strand, within RR42_RS15540 at 3358.125 kb on - strand, within RR42_RS15540 at 3358.125 kb on - strand, within RR42_RS15540 at 3358.125 kb on - strand, within RR42_RS15540 at 3358.181 kb on + strand, within RR42_RS15540 at 3358.181 kb on + strand, within RR42_RS15540 at 3358.385 kb on + strand at 3358.385 kb on + strand at 3358.385 kb on + strand at 3358.386 kb on - strand at 3358.386 kb on - strand at 3358.487 kb on - strand, within RR42_RS15545 at 3358.500 kb on + strand, within RR42_RS15545 at 3358.501 kb on - strand, within RR42_RS15545 at 3358.501 kb on - strand, within RR42_RS15545 at 3358.519 kb on + strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3359.093 kb on - strand, within RR42_RS15550 at 3359.255 kb on + strand, within RR42_RS15550 at 3359.255 kb on + strand, within RR42_RS15550 at 3359.255 kb on + strand, within RR42_RS15550 at 3359.256 kb on - strand, within RR42_RS15550 at 3359.256 kb on - strand, within RR42_RS15550 at 3359.256 kb on - strand, within RR42_RS15550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,355,970 - RR42_RS15530 0.76 +0.6 3,355,970 - RR42_RS15530 0.76 +1.7 3,356,042 + RR42_RS15530 0.83 +0.1 3,356,043 - RR42_RS15530 0.84 +0.4 3,356,043 - RR42_RS15530 0.84 -0.1 3,356,165 + +0.1 3,356,188 + -1.8 3,356,188 + +0.4 3,356,188 + -0.8 3,356,188 + +0.4 3,356,188 + -2.7 3,356,188 + -0.6 3,356,188 + +0.0 3,356,188 + +0.6 3,356,189 - +0.4 3,356,189 - +0.7 3,356,189 - -0.2 3,356,342 + RR42_RS15535 0.13 -0.2 3,356,342 + RR42_RS15535 0.13 -1.5 3,356,443 - RR42_RS15535 0.32 -0.4 3,356,446 + RR42_RS15535 0.32 -0.1 3,356,661 - RR42_RS15535 0.71 -2.4 3,356,715 - RR42_RS15535 0.81 -1.5 3,356,715 - RR42_RS15535 0.81 -0.2 3,356,796 + +0.2 3,356,796 + -0.4 3,356,797 - +0.4 3,356,797 - -1.2 3,357,057 - +0.1 3,357,249 - RR42_RS15540 0.23 -0.8 3,357,350 - RR42_RS15540 0.30 +0.2 3,357,577 - RR42_RS15540 0.46 -1.3 3,357,601 + RR42_RS15540 0.48 -1.1 3,357,602 - RR42_RS15540 0.48 -0.3 3,357,691 + RR42_RS15540 0.54 +0.0 3,357,691 + RR42_RS15540 0.54 +0.0 3,357,692 - RR42_RS15540 0.54 +0.3 3,357,835 - RR42_RS15540 0.64 +0.4 3,357,858 + RR42_RS15540 0.66 +0.4 3,357,859 - RR42_RS15540 0.66 +0.1 3,357,952 + RR42_RS15540 0.72 -2.0 3,357,953 - RR42_RS15540 0.72 -0.3 3,357,953 - RR42_RS15540 0.72 +0.6 3,358,057 + RR42_RS15540 0.80 -0.3 3,358,124 + RR42_RS15540 0.84 +1.2 3,358,124 + RR42_RS15540 0.84 +0.5 3,358,125 - RR42_RS15540 0.84 +0.1 3,358,125 - RR42_RS15540 0.84 -0.6 3,358,125 - RR42_RS15540 0.84 +0.5 3,358,181 + RR42_RS15540 0.88 +0.0 3,358,181 + RR42_RS15540 0.88 -2.1 3,358,385 + +0.1 3,358,385 + +0.4 3,358,385 + +0.8 3,358,386 - +1.3 3,358,386 - -0.1 3,358,487 - RR42_RS15545 0.17 +0.6 3,358,500 + RR42_RS15545 0.20 +0.6 3,358,501 - RR42_RS15545 0.20 -0.1 3,358,501 - RR42_RS15545 0.20 +0.9 3,358,519 + RR42_RS15545 0.24 -0.6 3,358,520 - RR42_RS15545 0.24 +0.2 3,358,520 - RR42_RS15545 0.24 +0.5 3,358,520 - RR42_RS15545 0.24 -0.9 3,358,520 - RR42_RS15545 0.24 -2.9 3,359,093 - RR42_RS15550 0.39 +0.5 3,359,255 + RR42_RS15550 0.67 +0.4 3,359,255 + RR42_RS15550 0.67 +0.5 3,359,255 + RR42_RS15550 0.67 +0.1 3,359,256 - RR42_RS15550 0.68 -0.8 3,359,256 - RR42_RS15550 0.68 -0.0 3,359,256 - RR42_RS15550 0.68 +0.1
Or see this region's nucleotide sequence