Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11590 and RR42_RS11595 are separated by 515 nucleotides RR42_RS11595 and RR42_RS11600 are separated by 607 nucleotides
RR42_RS11590: RR42_RS11590 - nitroreductase, at 2,481,069 to 2,481,641
_RS11590
RR42_RS11595: RR42_RS11595 - hypothetical protein, at 2,482,157 to 2,482,477
_RS11595
RR42_RS11600: RR42_RS11600 - flavin reductase, at 2,483,085 to 2,483,627
_RS11600
Position (kb)
2482
2483 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2481.203 kb on - strand, within RR42_RS11590 at 2481.221 kb on - strand, within RR42_RS11590 at 2481.239 kb on + strand, within RR42_RS11590 at 2481.242 kb on - strand, within RR42_RS11590 at 2481.242 kb on - strand, within RR42_RS11590 at 2481.242 kb on - strand, within RR42_RS11590 at 2481.242 kb on - strand, within RR42_RS11590 at 2481.242 kb on - strand, within RR42_RS11590 at 2481.242 kb on - strand, within RR42_RS11590 at 2481.295 kb on + strand, within RR42_RS11590 at 2481.296 kb on - strand, within RR42_RS11590 at 2481.325 kb on + strand, within RR42_RS11590 at 2481.325 kb on + strand, within RR42_RS11590 at 2481.325 kb on + strand, within RR42_RS11590 at 2481.326 kb on - strand, within RR42_RS11590 at 2481.326 kb on - strand, within RR42_RS11590 at 2481.326 kb on - strand, within RR42_RS11590 at 2481.326 kb on - strand, within RR42_RS11590 at 2481.403 kb on + strand, within RR42_RS11590 at 2481.403 kb on + strand, within RR42_RS11590 at 2481.403 kb on + strand, within RR42_RS11590 at 2481.403 kb on + strand, within RR42_RS11590 at 2481.404 kb on - strand, within RR42_RS11590 at 2481.404 kb on - strand, within RR42_RS11590 at 2481.404 kb on - strand, within RR42_RS11590 at 2481.449 kb on + strand, within RR42_RS11590 at 2481.525 kb on + strand, within RR42_RS11590 at 2481.720 kb on + strand at 2481.721 kb on - strand at 2481.728 kb on + strand at 2481.729 kb on - strand at 2481.792 kb on + strand at 2481.793 kb on - strand at 2481.884 kb on - strand at 2481.884 kb on - strand at 2482.063 kb on + strand at 2482.064 kb on - strand at 2482.158 kb on + strand at 2482.158 kb on + strand at 2482.201 kb on + strand, within RR42_RS11595 at 2482.202 kb on - strand, within RR42_RS11595 at 2482.218 kb on + strand, within RR42_RS11595 at 2482.316 kb on + strand, within RR42_RS11595 at 2482.317 kb on - strand, within RR42_RS11595 at 2482.371 kb on - strand, within RR42_RS11595 at 2482.371 kb on - strand, within RR42_RS11595 at 2482.398 kb on + strand, within RR42_RS11595 at 2482.398 kb on + strand, within RR42_RS11595 at 2482.423 kb on - strand, within RR42_RS11595 at 2482.449 kb on + strand at 2482.451 kb on + strand at 2482.452 kb on - strand at 2482.461 kb on - strand at 2482.473 kb on + strand at 2482.537 kb on - strand at 2482.537 kb on - strand at 2482.537 kb on - strand at 2482.593 kb on + strand at 2482.593 kb on + strand at 2482.598 kb on + strand at 2482.663 kb on + strand at 2482.745 kb on + strand at 2482.781 kb on + strand at 2482.820 kb on + strand at 2482.887 kb on + strand at 2482.976 kb on + strand at 2483.010 kb on + strand at 2483.010 kb on + strand at 2483.011 kb on - strand at 2483.011 kb on - strand at 2483.080 kb on - strand at 2483.080 kb on - strand at 2483.080 kb on - strand at 2483.082 kb on + strand at 2483.087 kb on - strand at 2483.156 kb on + strand, within RR42_RS11600 at 2483.227 kb on - strand, within RR42_RS11600 at 2483.275 kb on - strand, within RR42_RS11600 at 2483.275 kb on - strand, within RR42_RS11600 at 2483.275 kb on - strand, within RR42_RS11600 at 2483.314 kb on + strand, within RR42_RS11600 at 2483.315 kb on - strand, within RR42_RS11600 at 2483.361 kb on - strand, within RR42_RS11600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 2,481,203 - RR42_RS11590 0.23 +0.1 2,481,221 - RR42_RS11590 0.27 -0.0 2,481,239 + RR42_RS11590 0.30 +0.9 2,481,242 - RR42_RS11590 0.30 +0.1 2,481,242 - RR42_RS11590 0.30 +0.1 2,481,242 - RR42_RS11590 0.30 -0.3 2,481,242 - RR42_RS11590 0.30 +0.9 2,481,242 - RR42_RS11590 0.30 +0.8 2,481,242 - RR42_RS11590 0.30 -0.0 2,481,295 + RR42_RS11590 0.39 +0.1 2,481,296 - RR42_RS11590 0.40 +0.3 2,481,325 + RR42_RS11590 0.45 -0.1 2,481,325 + RR42_RS11590 0.45 -0.2 2,481,325 + RR42_RS11590 0.45 +0.1 2,481,326 - RR42_RS11590 0.45 -1.6 2,481,326 - RR42_RS11590 0.45 +0.3 2,481,326 - RR42_RS11590 0.45 +0.4 2,481,326 - RR42_RS11590 0.45 -0.7 2,481,403 + RR42_RS11590 0.58 +0.1 2,481,403 + RR42_RS11590 0.58 +0.6 2,481,403 + RR42_RS11590 0.58 -2.1 2,481,403 + RR42_RS11590 0.58 -2.8 2,481,404 - RR42_RS11590 0.58 +0.2 2,481,404 - RR42_RS11590 0.58 -1.6 2,481,404 - RR42_RS11590 0.58 -0.3 2,481,449 + RR42_RS11590 0.66 +0.4 2,481,525 + RR42_RS11590 0.80 +0.3 2,481,720 + +0.1 2,481,721 - +0.6 2,481,728 + +0.3 2,481,729 - -0.1 2,481,792 + +1.3 2,481,793 - -0.8 2,481,884 - -0.8 2,481,884 - +0.1 2,482,063 + -1.0 2,482,064 - -0.4 2,482,158 + +0.5 2,482,158 + -0.0 2,482,201 + RR42_RS11595 0.14 +0.8 2,482,202 - RR42_RS11595 0.14 -0.1 2,482,218 + RR42_RS11595 0.19 +0.4 2,482,316 + RR42_RS11595 0.50 -0.4 2,482,317 - RR42_RS11595 0.50 +0.8 2,482,371 - RR42_RS11595 0.67 -0.1 2,482,371 - RR42_RS11595 0.67 -0.2 2,482,398 + RR42_RS11595 0.75 +0.9 2,482,398 + RR42_RS11595 0.75 +0.3 2,482,423 - RR42_RS11595 0.83 -0.1 2,482,449 + -2.2 2,482,451 + -0.6 2,482,452 - +0.4 2,482,461 - -0.1 2,482,473 + +0.7 2,482,537 - +0.8 2,482,537 - -0.1 2,482,537 - -0.1 2,482,593 + +0.2 2,482,593 + +0.6 2,482,598 + +0.2 2,482,663 + +0.0 2,482,745 + +1.0 2,482,781 + +0.5 2,482,820 + +0.2 2,482,887 + -0.2 2,482,976 + -0.9 2,483,010 + +0.2 2,483,010 + -0.5 2,483,011 - -0.3 2,483,011 - -0.2 2,483,080 - -0.3 2,483,080 - +0.6 2,483,080 - +0.2 2,483,082 + +0.2 2,483,087 - +0.1 2,483,156 + RR42_RS11600 0.13 -0.3 2,483,227 - RR42_RS11600 0.26 -0.2 2,483,275 - RR42_RS11600 0.35 -2.5 2,483,275 - RR42_RS11600 0.35 -0.0 2,483,275 - RR42_RS11600 0.35 -0.4 2,483,314 + RR42_RS11600 0.42 +0.0 2,483,315 - RR42_RS11600 0.42 -0.6 2,483,361 - RR42_RS11600 0.51 -3.4
Or see this region's nucleotide sequence