Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08430 and RR42_RS08435 are separated by 48 nucleotides RR42_RS08435 and RR42_RS08440 overlap by 4 nucleotides RR42_RS08440 and RR42_RS08445 overlap by 11 nucleotides
RR42_RS08430: RR42_RS08430 - hypothetical protein, at 1,779,634 to 1,780,215
_RS08430
RR42_RS08435: RR42_RS08435 - hypothetical protein, at 1,780,264 to 1,780,749
_RS08435
RR42_RS08440: RR42_RS08440 - ATP synthase I, at 1,780,746 to 1,781,387
_RS08440
RR42_RS08445: RR42_RS08445 - hypothetical protein, at 1,781,377 to 1,781,799
_RS08445
Position (kb)
1780
1781 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1779.382 kb on - strand at 1779.382 kb on - strand at 1779.470 kb on + strand at 1779.471 kb on - strand at 1779.511 kb on + strand at 1779.514 kb on + strand at 1779.514 kb on + strand at 1779.520 kb on + strand at 1779.565 kb on + strand at 1779.679 kb on + strand at 1779.680 kb on - strand at 1779.681 kb on + strand at 1779.682 kb on - strand at 1779.682 kb on - strand at 1779.682 kb on - strand at 1779.778 kb on + strand, within RR42_RS08430 at 1779.779 kb on - strand, within RR42_RS08430 at 1779.875 kb on + strand, within RR42_RS08430 at 1779.930 kb on + strand, within RR42_RS08430 at 1779.930 kb on + strand, within RR42_RS08430 at 1779.931 kb on - strand, within RR42_RS08430 at 1779.931 kb on - strand, within RR42_RS08430 at 1779.931 kb on - strand, within RR42_RS08430 at 1779.958 kb on - strand, within RR42_RS08430 at 1780.010 kb on + strand, within RR42_RS08430 at 1780.014 kb on - strand, within RR42_RS08430 at 1780.039 kb on + strand, within RR42_RS08430 at 1780.056 kb on - strand, within RR42_RS08430 at 1780.066 kb on + strand, within RR42_RS08430 at 1780.083 kb on + strand, within RR42_RS08430 at 1780.083 kb on + strand, within RR42_RS08430 at 1780.084 kb on - strand, within RR42_RS08430 at 1780.084 kb on - strand, within RR42_RS08430 at 1780.167 kb on + strand at 1780.168 kb on - strand at 1780.188 kb on - strand at 1780.221 kb on - strand at 1780.234 kb on + strand at 1780.235 kb on - strand at 1780.235 kb on - strand at 1780.235 kb on - strand at 1780.348 kb on + strand, within RR42_RS08435 at 1780.349 kb on - strand, within RR42_RS08435 at 1780.349 kb on - strand, within RR42_RS08435 at 1780.350 kb on + strand, within RR42_RS08435 at 1780.350 kb on + strand, within RR42_RS08435 at 1780.353 kb on + strand, within RR42_RS08435 at 1780.353 kb on + strand, within RR42_RS08435 at 1780.363 kb on - strand, within RR42_RS08435 at 1780.395 kb on + strand, within RR42_RS08435 at 1780.395 kb on + strand, within RR42_RS08435 at 1780.396 kb on - strand, within RR42_RS08435 at 1780.417 kb on - strand, within RR42_RS08435 at 1780.480 kb on - strand, within RR42_RS08435 at 1780.609 kb on + strand, within RR42_RS08435 at 1780.637 kb on - strand, within RR42_RS08435 at 1780.967 kb on - strand, within RR42_RS08440 at 1781.068 kb on + strand, within RR42_RS08440 at 1781.068 kb on + strand, within RR42_RS08440 at 1781.069 kb on - strand, within RR42_RS08440 at 1781.069 kb on - strand, within RR42_RS08440 at 1781.069 kb on - strand, within RR42_RS08440 at 1781.081 kb on - strand, within RR42_RS08440 at 1781.312 kb on - strand, within RR42_RS08440 at 1781.374 kb on + strand at 1781.374 kb on + strand at 1781.375 kb on - strand at 1781.375 kb on - strand at 1781.375 kb on - strand at 1781.375 kb on - strand at 1781.378 kb on + strand at 1781.378 kb on + strand at 1781.379 kb on - strand at 1781.379 kb on - strand at 1781.455 kb on - strand, within RR42_RS08445 at 1781.699 kb on + strand, within RR42_RS08445 at 1781.699 kb on + strand, within RR42_RS08445 at 1781.700 kb on - strand, within RR42_RS08445 at 1781.726 kb on + strand, within RR42_RS08445 at 1781.727 kb on - strand, within RR42_RS08445 at 1781.727 kb on - strand, within RR42_RS08445
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,779,382 - +1.7 1,779,382 - +0.6 1,779,470 + +0.1 1,779,471 - +0.5 1,779,511 + -0.1 1,779,514 + +0.2 1,779,514 + +0.3 1,779,520 + -2.7 1,779,565 + +0.9 1,779,679 + +0.2 1,779,680 - +0.8 1,779,681 + -0.8 1,779,682 - +1.0 1,779,682 - +0.5 1,779,682 - +0.5 1,779,778 + RR42_RS08430 0.25 -0.5 1,779,779 - RR42_RS08430 0.25 +1.1 1,779,875 + RR42_RS08430 0.41 -0.3 1,779,930 + RR42_RS08430 0.51 -0.3 1,779,930 + RR42_RS08430 0.51 -1.8 1,779,931 - RR42_RS08430 0.51 -0.8 1,779,931 - RR42_RS08430 0.51 +0.2 1,779,931 - RR42_RS08430 0.51 +0.4 1,779,958 - RR42_RS08430 0.56 +1.4 1,780,010 + RR42_RS08430 0.65 +0.4 1,780,014 - RR42_RS08430 0.65 +1.8 1,780,039 + RR42_RS08430 0.70 +0.7 1,780,056 - RR42_RS08430 0.73 +0.1 1,780,066 + RR42_RS08430 0.74 +1.3 1,780,083 + RR42_RS08430 0.77 +0.6 1,780,083 + RR42_RS08430 0.77 +1.0 1,780,084 - RR42_RS08430 0.77 -0.3 1,780,084 - RR42_RS08430 0.77 +0.2 1,780,167 + +0.3 1,780,168 - +0.2 1,780,188 - +0.7 1,780,221 - +0.8 1,780,234 + +0.5 1,780,235 - +0.7 1,780,235 - -0.2 1,780,235 - +0.3 1,780,348 + RR42_RS08435 0.17 +0.7 1,780,349 - RR42_RS08435 0.17 +1.1 1,780,349 - RR42_RS08435 0.17 +0.9 1,780,350 + RR42_RS08435 0.18 +1.0 1,780,350 + RR42_RS08435 0.18 +0.3 1,780,353 + RR42_RS08435 0.18 -0.2 1,780,353 + RR42_RS08435 0.18 +0.6 1,780,363 - RR42_RS08435 0.20 -1.9 1,780,395 + RR42_RS08435 0.27 -1.2 1,780,395 + RR42_RS08435 0.27 +0.3 1,780,396 - RR42_RS08435 0.27 +0.4 1,780,417 - RR42_RS08435 0.31 +0.8 1,780,480 - RR42_RS08435 0.44 +1.1 1,780,609 + RR42_RS08435 0.71 -0.8 1,780,637 - RR42_RS08435 0.77 +1.1 1,780,967 - RR42_RS08440 0.34 +1.0 1,781,068 + RR42_RS08440 0.50 +0.3 1,781,068 + RR42_RS08440 0.50 +0.6 1,781,069 - RR42_RS08440 0.50 +1.0 1,781,069 - RR42_RS08440 0.50 +0.2 1,781,069 - RR42_RS08440 0.50 +1.1 1,781,081 - RR42_RS08440 0.52 +0.5 1,781,312 - RR42_RS08440 0.88 +1.2 1,781,374 + -0.2 1,781,374 + -0.3 1,781,375 - +1.7 1,781,375 - +0.2 1,781,375 - +1.2 1,781,375 - -0.1 1,781,378 + +0.3 1,781,378 + -0.7 1,781,379 - +0.6 1,781,379 - -0.0 1,781,455 - RR42_RS08445 0.18 +0.0 1,781,699 + RR42_RS08445 0.76 +1.1 1,781,699 + RR42_RS08445 0.76 -0.1 1,781,700 - RR42_RS08445 0.76 +2.7 1,781,726 + RR42_RS08445 0.83 -2.8 1,781,727 - RR42_RS08445 0.83 +0.4 1,781,727 - RR42_RS08445 0.83 +0.0
Or see this region's nucleotide sequence