Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06120 and RR42_RS06125 are separated by 325 nucleotides RR42_RS06125 and RR42_RS06130 are separated by 187 nucleotides RR42_RS06130 and RR42_RS06135 are separated by 262 nucleotides
RR42_RS06120: RR42_RS06120 - transcriptional regulator, at 1,344,095 to 1,345,033
_RS06120
RR42_RS06125: RR42_RS06125 - phosphogluconate dehydratase, at 1,345,359 to 1,347,245
_RS06125
RR42_RS06130: RR42_RS06130 - Gluconokinase (EC 2.7.1.12) (from data) , at 1,347,433 to 1,347,942
_RS06130
RR42_RS06135: RR42_RS06135 - hypothetical protein, at 1,348,205 to 1,349,677
_RS06135
Position (kb)
1345
1346
1347
1348 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1344.640 kb on - strand, within RR42_RS06120 at 1344.970 kb on - strand at 1344.970 kb on - strand at 1345.008 kb on + strand at 1345.009 kb on - strand at 1345.009 kb on - strand at 1345.009 kb on - strand at 1345.119 kb on - strand at 1345.122 kb on + strand at 1345.122 kb on + strand at 1345.132 kb on - strand at 1345.135 kb on - strand at 1345.187 kb on + strand at 1345.212 kb on - strand at 1345.261 kb on - strand at 1345.262 kb on + strand at 1345.262 kb on + strand at 1345.263 kb on - strand at 1345.305 kb on + strand at 1345.306 kb on - strand at 1345.306 kb on - strand at 1345.306 kb on - strand at 1345.306 kb on - strand at 1345.438 kb on - strand at 1345.438 kb on - strand at 1345.590 kb on + strand, within RR42_RS06125 at 1345.591 kb on - strand, within RR42_RS06125 at 1346.034 kb on - strand, within RR42_RS06125 at 1346.034 kb on - strand, within RR42_RS06125 at 1346.034 kb on - strand, within RR42_RS06125 at 1346.034 kb on - strand, within RR42_RS06125 at 1346.041 kb on - strand, within RR42_RS06125 at 1346.091 kb on - strand, within RR42_RS06125 at 1347.244 kb on - strand at 1347.244 kb on - strand at 1347.439 kb on + strand at 1347.439 kb on + strand at 1347.439 kb on + strand at 1347.439 kb on + strand at 1347.439 kb on + strand at 1347.439 kb on + strand at 1347.439 kb on + strand at 1347.439 kb on + strand at 1347.440 kb on - strand at 1347.663 kb on + strand, within RR42_RS06130 at 1347.734 kb on - strand, within RR42_RS06130 at 1347.734 kb on - strand, within RR42_RS06130 at 1347.734 kb on - strand, within RR42_RS06130 at 1347.734 kb on - strand, within RR42_RS06130 at 1347.989 kb on + strand at 1347.990 kb on - strand at 1348.039 kb on + strand at 1348.040 kb on - strand at 1348.040 kb on - strand at 1348.040 kb on - strand at 1348.040 kb on - strand at 1348.040 kb on - strand at 1348.040 kb on - strand at 1348.040 kb on - strand at 1348.040 kb on - strand at 1348.043 kb on + strand at 1348.043 kb on + strand at 1348.044 kb on - strand at 1348.044 kb on - strand at 1348.048 kb on + strand at 1348.048 kb on + strand at 1348.049 kb on - strand at 1348.049 kb on - strand at 1348.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,344,640 - RR42_RS06120 0.58 -1.1 1,344,970 - -0.2 1,344,970 - +0.1 1,345,008 + -0.5 1,345,009 - -0.7 1,345,009 - +0.6 1,345,009 - -1.3 1,345,119 - +0.1 1,345,122 + +0.2 1,345,122 + +0.4 1,345,132 - +0.3 1,345,135 - -0.7 1,345,187 + -0.8 1,345,212 - -0.5 1,345,261 - +0.4 1,345,262 + +0.3 1,345,262 + -1.1 1,345,263 - -0.5 1,345,305 + -0.7 1,345,306 - +0.7 1,345,306 - -1.8 1,345,306 - -0.4 1,345,306 - -0.2 1,345,438 - -1.2 1,345,438 - -1.4 1,345,590 + RR42_RS06125 0.12 -2.1 1,345,591 - RR42_RS06125 0.12 -1.4 1,346,034 - RR42_RS06125 0.36 -0.2 1,346,034 - RR42_RS06125 0.36 -0.7 1,346,034 - RR42_RS06125 0.36 -1.8 1,346,034 - RR42_RS06125 0.36 -0.7 1,346,041 - RR42_RS06125 0.36 +0.2 1,346,091 - RR42_RS06125 0.39 -1.4 1,347,244 - +1.2 1,347,244 - +0.4 1,347,439 + +0.6 1,347,439 + -0.1 1,347,439 + +0.3 1,347,439 + -1.2 1,347,439 + -0.1 1,347,439 + +0.4 1,347,439 + -0.7 1,347,439 + +0.1 1,347,440 - +1.8 1,347,663 + RR42_RS06130 0.45 +0.1 1,347,734 - RR42_RS06130 0.59 +0.0 1,347,734 - RR42_RS06130 0.59 +0.4 1,347,734 - RR42_RS06130 0.59 -0.1 1,347,734 - RR42_RS06130 0.59 +0.7 1,347,989 + +0.2 1,347,990 - -0.3 1,348,039 + +0.8 1,348,040 - -0.5 1,348,040 - -0.6 1,348,040 - -0.2 1,348,040 - -0.0 1,348,040 - -0.2 1,348,040 - +0.3 1,348,040 - +0.3 1,348,040 - -1.9 1,348,043 + -1.3 1,348,043 + -3.9 1,348,044 - +0.1 1,348,044 - +0.9 1,348,048 + -1.3 1,348,048 + -0.7 1,348,049 - +0.3 1,348,049 - +0.6 1,348,051 - +0.4
Or see this region's nucleotide sequence