Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05885 and RR42_RS05890 are separated by 258 nucleotides RR42_RS05890 and RR42_RS05895 overlap by 4 nucleotides RR42_RS05895 and RR42_RS05900 are separated by 71 nucleotides
RR42_RS05885: RR42_RS05885 - hypothetical protein, at 1,291,737 to 1,295,921
_RS05885
RR42_RS05890: RR42_RS05890 - glutamine-synthetase adenylyltransferase, at 1,296,180 to 1,299,140
_RS05890
RR42_RS05895: RR42_RS05895 - rhombosortase, at 1,299,137 to 1,299,772
_RS05895
RR42_RS05900: RR42_RS05900 - peptidase, at 1,299,844 to 1,301,529
_RS05900
Position (kb)
1296
1297
1298
1299
1300 Strain fitness (log2 ratio)
-2
-1
0
1 at 1295.248 kb on + strand, within RR42_RS05885 at 1295.248 kb on + strand, within RR42_RS05885 at 1295.248 kb on + strand, within RR42_RS05885 at 1295.249 kb on - strand, within RR42_RS05885 at 1295.249 kb on - strand, within RR42_RS05885 at 1295.250 kb on - strand, within RR42_RS05885 at 1295.272 kb on - strand, within RR42_RS05885 at 1295.713 kb on + strand at 1295.713 kb on + strand at 1295.713 kb on + strand at 1295.714 kb on - strand at 1295.714 kb on - strand at 1295.714 kb on - strand at 1295.714 kb on - strand at 1295.717 kb on + strand at 1295.718 kb on - strand at 1296.090 kb on + strand at 1296.090 kb on + strand at 1296.091 kb on - strand at 1296.091 kb on - strand at 1296.091 kb on - strand at 1296.091 kb on - strand at 1296.094 kb on - strand at 1296.094 kb on - strand at 1296.173 kb on - strand at 1296.173 kb on - strand at 1296.372 kb on + strand at 1296.372 kb on + strand at 1296.373 kb on - strand at 1296.385 kb on - strand at 1296.385 kb on - strand at 1296.385 kb on - strand at 1296.393 kb on + strand at 1296.393 kb on + strand at 1296.394 kb on - strand at 1296.660 kb on + strand, within RR42_RS05890 at 1296.699 kb on + strand, within RR42_RS05890 at 1296.700 kb on - strand, within RR42_RS05890 at 1296.816 kb on + strand, within RR42_RS05890 at 1296.816 kb on + strand, within RR42_RS05890 at 1296.817 kb on - strand, within RR42_RS05890 at 1296.871 kb on - strand, within RR42_RS05890 at 1296.871 kb on - strand, within RR42_RS05890 at 1296.871 kb on - strand, within RR42_RS05890 at 1296.930 kb on + strand, within RR42_RS05890 at 1296.930 kb on + strand, within RR42_RS05890 at 1296.931 kb on - strand, within RR42_RS05890 at 1296.931 kb on - strand, within RR42_RS05890 at 1297.011 kb on + strand, within RR42_RS05890 at 1297.011 kb on + strand, within RR42_RS05890 at 1297.044 kb on + strand, within RR42_RS05890 at 1297.044 kb on + strand, within RR42_RS05890 at 1297.045 kb on - strand, within RR42_RS05890 at 1297.488 kb on + strand, within RR42_RS05890 at 1297.488 kb on + strand, within RR42_RS05890 at 1297.488 kb on + strand, within RR42_RS05890 at 1298.025 kb on + strand, within RR42_RS05890 at 1298.025 kb on + strand, within RR42_RS05890 at 1298.025 kb on + strand, within RR42_RS05890 at 1298.419 kb on - strand, within RR42_RS05890 at 1298.451 kb on + strand, within RR42_RS05890 at 1298.461 kb on - strand, within RR42_RS05890 at 1298.859 kb on + strand at 1298.859 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1299.195 kb on + strand at 1299.229 kb on + strand, within RR42_RS05895 at 1299.229 kb on + strand, within RR42_RS05895 at 1299.263 kb on + strand, within RR42_RS05895 at 1299.721 kb on - strand at 1299.771 kb on - strand at 1300.074 kb on - strand, within RR42_RS05900 at 1300.074 kb on - strand, within RR42_RS05900 at 1300.074 kb on - strand, within RR42_RS05900 at 1300.074 kb on - strand, within RR42_RS05900
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,295,248 + RR42_RS05885 0.84 +0.6 1,295,248 + RR42_RS05885 0.84 -2.5 1,295,248 + RR42_RS05885 0.84 -0.6 1,295,249 - RR42_RS05885 0.84 -0.9 1,295,249 - RR42_RS05885 0.84 -0.1 1,295,250 - RR42_RS05885 0.84 -0.3 1,295,272 - RR42_RS05885 0.84 +0.1 1,295,713 + -0.9 1,295,713 + -0.3 1,295,713 + -2.5 1,295,714 - -2.2 1,295,714 - -1.2 1,295,714 - -0.9 1,295,714 - +0.4 1,295,717 + -1.2 1,295,718 - +0.6 1,296,090 + -0.9 1,296,090 + -0.5 1,296,091 - +0.9 1,296,091 - -0.6 1,296,091 - +1.0 1,296,091 - +1.2 1,296,094 - +0.4 1,296,094 - -0.4 1,296,173 - -1.1 1,296,173 - +0.2 1,296,372 + +0.6 1,296,372 + -0.6 1,296,373 - -1.9 1,296,385 - +0.1 1,296,385 - +0.7 1,296,385 - -0.4 1,296,393 + +0.5 1,296,393 + -0.9 1,296,394 - -0.3 1,296,660 + RR42_RS05890 0.16 +0.3 1,296,699 + RR42_RS05890 0.18 -0.6 1,296,700 - RR42_RS05890 0.18 +0.2 1,296,816 + RR42_RS05890 0.21 +0.4 1,296,816 + RR42_RS05890 0.21 -0.2 1,296,817 - RR42_RS05890 0.22 -0.9 1,296,871 - RR42_RS05890 0.23 -1.2 1,296,871 - RR42_RS05890 0.23 -2.0 1,296,871 - RR42_RS05890 0.23 -0.6 1,296,930 + RR42_RS05890 0.25 +0.6 1,296,930 + RR42_RS05890 0.25 -0.4 1,296,931 - RR42_RS05890 0.25 -0.7 1,296,931 - RR42_RS05890 0.25 -0.4 1,297,011 + RR42_RS05890 0.28 -0.7 1,297,011 + RR42_RS05890 0.28 -0.3 1,297,044 + RR42_RS05890 0.29 +0.0 1,297,044 + RR42_RS05890 0.29 -0.8 1,297,045 - RR42_RS05890 0.29 +0.7 1,297,488 + RR42_RS05890 0.44 +0.2 1,297,488 + RR42_RS05890 0.44 -0.1 1,297,488 + RR42_RS05890 0.44 -0.9 1,298,025 + RR42_RS05890 0.62 -0.1 1,298,025 + RR42_RS05890 0.62 -0.4 1,298,025 + RR42_RS05890 0.62 -1.4 1,298,419 - RR42_RS05890 0.76 -0.7 1,298,451 + RR42_RS05890 0.77 -0.2 1,298,461 - RR42_RS05890 0.77 -2.6 1,298,859 + -0.0 1,298,859 - +0.7 1,298,860 - -2.0 1,298,860 - -0.0 1,298,860 - +0.1 1,298,860 - -0.2 1,298,860 - -1.0 1,299,195 + -1.0 1,299,229 + RR42_RS05895 0.14 -1.0 1,299,229 + RR42_RS05895 0.14 -0.2 1,299,263 + RR42_RS05895 0.20 -1.1 1,299,721 - +1.2 1,299,771 - -0.5 1,300,074 - RR42_RS05900 0.14 +0.5 1,300,074 - RR42_RS05900 0.14 -0.0 1,300,074 - RR42_RS05900 0.14 +0.6 1,300,074 - RR42_RS05900 0.14 -0.3
Or see this region's nucleotide sequence