Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02855 and RR42_RS02860 are separated by 94 nucleotides RR42_RS02860 and RR42_RS02865 are separated by 123 nucleotides RR42_RS02865 and RR42_RS02870 are separated by 307 nucleotides
RR42_RS02855: RR42_RS02855 - dimethylallyladenosine tRNA methylthiotransferase, at 636,191 to 637,543
_RS02855
RR42_RS02860: RR42_RS02860 - hypothetical protein, at 637,638 to 638,018
_RS02860
RR42_RS02865: RR42_RS02865 - chloride channel protein, at 638,142 to 639,830
_RS02865
RR42_RS02870: RR42_RS02870 - RNA helicase, at 640,138 to 641,826
_RS02870
Position (kb)
638
639
640 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 637.475 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.634 kb on - strand at 637.634 kb on - strand at 637.639 kb on + strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.763 kb on + strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 638.106 kb on + strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.143 kb on + strand at 638.144 kb on - strand at 638.144 kb on - strand at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.414 kb on - strand, within RR42_RS02865 at 638.419 kb on + strand, within RR42_RS02865 at 638.575 kb on + strand, within RR42_RS02865 at 638.575 kb on + strand, within RR42_RS02865 at 638.575 kb on + strand, within RR42_RS02865 at 638.576 kb on - strand, within RR42_RS02865 at 638.608 kb on + strand, within RR42_RS02865 at 638.609 kb on - strand, within RR42_RS02865 at 638.612 kb on - strand, within RR42_RS02865 at 638.902 kb on - strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 639.195 kb on + strand, within RR42_RS02865 at 639.196 kb on - strand, within RR42_RS02865 at 639.196 kb on - strand, within RR42_RS02865 at 639.196 kb on - strand, within RR42_RS02865 at 639.196 kb on - strand, within RR42_RS02865 at 639.196 kb on - strand, within RR42_RS02865 at 639.557 kb on + strand, within RR42_RS02865 at 639.557 kb on + strand, within RR42_RS02865 at 639.558 kb on - strand, within RR42_RS02865 at 639.558 kb on - strand, within RR42_RS02865 at 639.559 kb on + strand, within RR42_RS02865 at 639.559 kb on + strand, within RR42_RS02865 at 639.560 kb on - strand, within RR42_RS02865 at 639.678 kb on + strand at 639.689 kb on + strand at 639.789 kb on - strand at 639.996 kb on - strand at 640.000 kb on + strand at 640.001 kb on - strand at 640.173 kb on + strand at 640.173 kb on + strand at 640.174 kb on - strand at 640.174 kb on - strand at 640.200 kb on + strand at 640.200 kb on + strand at 640.200 kb on + strand at 640.200 kb on + strand at 640.201 kb on - strand at 640.201 kb on - strand at 640.201 kb on - strand at 640.201 kb on - strand at 640.201 kb on - strand at 640.437 kb on + strand, within RR42_RS02870 at 640.441 kb on + strand, within RR42_RS02870 at 640.441 kb on + strand, within RR42_RS02870 at 640.442 kb on - strand, within RR42_RS02870 at 640.792 kb on - strand, within RR42_RS02870 at 640.823 kb on - strand, within RR42_RS02870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 637,475 - +0.9 637,519 - -0.1 637,519 - -0.9 637,519 - -0.1 637,634 - -0.0 637,634 - -0.3 637,639 + -1.9 637,640 - +1.0 637,640 - +0.6 637,640 - -0.2 637,640 - -0.3 637,763 + RR42_RS02860 0.33 -0.8 637,960 - RR42_RS02860 0.85 +1.8 637,960 - RR42_RS02860 0.85 -0.8 638,106 + -1.3 638,107 - +0.7 638,107 - +0.3 638,107 - -0.3 638,107 - +0.3 638,110 - +0.3 638,110 - +0.7 638,110 - -0.5 638,110 - +0.4 638,110 - +0.1 638,143 + +0.3 638,144 - +0.5 638,144 - +0.9 638,413 + RR42_RS02865 0.16 +1.0 638,413 + RR42_RS02865 0.16 -0.8 638,413 + RR42_RS02865 0.16 -0.0 638,413 + RR42_RS02865 0.16 +0.3 638,414 - RR42_RS02865 0.16 -0.2 638,419 + RR42_RS02865 0.16 -0.2 638,575 + RR42_RS02865 0.26 +0.0 638,575 + RR42_RS02865 0.26 +0.1 638,575 + RR42_RS02865 0.26 +0.3 638,576 - RR42_RS02865 0.26 -0.6 638,608 + RR42_RS02865 0.28 +0.3 638,609 - RR42_RS02865 0.28 -1.1 638,612 - RR42_RS02865 0.28 +0.6 638,902 - RR42_RS02865 0.45 +1.3 638,941 + RR42_RS02865 0.47 +0.2 638,941 + RR42_RS02865 0.47 +0.4 638,941 + RR42_RS02865 0.47 -1.0 638,941 + RR42_RS02865 0.47 -0.9 638,942 - RR42_RS02865 0.47 -1.7 638,942 - RR42_RS02865 0.47 +1.1 638,942 - RR42_RS02865 0.47 -0.1 638,942 - RR42_RS02865 0.47 -0.5 638,942 - RR42_RS02865 0.47 +0.9 638,942 - RR42_RS02865 0.47 -0.0 639,195 + RR42_RS02865 0.62 -0.3 639,196 - RR42_RS02865 0.62 +0.3 639,196 - RR42_RS02865 0.62 +1.6 639,196 - RR42_RS02865 0.62 +1.0 639,196 - RR42_RS02865 0.62 +0.4 639,196 - RR42_RS02865 0.62 +1.6 639,557 + RR42_RS02865 0.84 +0.4 639,557 + RR42_RS02865 0.84 +1.2 639,558 - RR42_RS02865 0.84 +2.0 639,558 - RR42_RS02865 0.84 +1.3 639,559 + RR42_RS02865 0.84 +0.9 639,559 + RR42_RS02865 0.84 -0.0 639,560 - RR42_RS02865 0.84 +1.0 639,678 + -0.4 639,689 + +1.3 639,789 - -0.6 639,996 - +0.2 640,000 + +1.5 640,001 - -0.3 640,173 + +0.7 640,173 + +1.1 640,174 - -1.0 640,174 - +0.1 640,200 + +1.1 640,200 + -0.7 640,200 + -0.0 640,200 + -0.2 640,201 - +0.5 640,201 - +0.4 640,201 - +0.7 640,201 - -1.1 640,201 - +1.4 640,437 + RR42_RS02870 0.18 +1.0 640,441 + RR42_RS02870 0.18 -0.1 640,441 + RR42_RS02870 0.18 -0.1 640,442 - RR42_RS02870 0.18 +0.5 640,792 - RR42_RS02870 0.39 -2.7 640,823 - RR42_RS02870 0.41 +2.2
Or see this region's nucleotide sequence