Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02130 and RR42_RS02135 are separated by 28 nucleotides RR42_RS02135 and RR42_RS02140 are separated by 111 nucleotides RR42_RS02140 and RR42_RS02145 are separated by 5 nucleotides
RR42_RS02130: RR42_RS02130 - membrane protein, at 472,170 to 473,855
_RS02130
RR42_RS02135: RR42_RS02135 - membrane protein, at 473,884 to 474,369
_RS02135
RR42_RS02140: RR42_RS02140 - hypothetical protein, at 474,481 to 474,831
_RS02140
RR42_RS02145: RR42_RS02145 - excinuclease ABC subunit A, at 474,837 to 477,701
_RS02145
Position (kb)
474
475 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 473.631 kb on + strand, within RR42_RS02130 at 473.632 kb on - strand, within RR42_RS02130 at 473.639 kb on + strand, within RR42_RS02130 at 473.640 kb on - strand, within RR42_RS02130 at 473.754 kb on + strand at 473.784 kb on - strand at 473.865 kb on - strand at 473.885 kb on + strand at 473.885 kb on + strand at 473.885 kb on + strand at 473.887 kb on + strand at 473.887 kb on + strand at 473.887 kb on + strand at 473.887 kb on + strand at 473.888 kb on - strand at 473.888 kb on - strand at 473.888 kb on - strand at 473.888 kb on - strand at 473.895 kb on - strand at 474.010 kb on + strand, within RR42_RS02135 at 474.010 kb on + strand, within RR42_RS02135 at 474.011 kb on - strand, within RR42_RS02135 at 474.011 kb on - strand, within RR42_RS02135 at 474.011 kb on - strand, within RR42_RS02135 at 474.055 kb on + strand, within RR42_RS02135 at 474.056 kb on - strand, within RR42_RS02135 at 474.235 kb on + strand, within RR42_RS02135 at 474.236 kb on - strand, within RR42_RS02135 at 474.355 kb on + strand at 474.355 kb on + strand at 474.356 kb on - strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.443 kb on + strand at 474.444 kb on - strand at 474.483 kb on - strand at 474.611 kb on - strand, within RR42_RS02140 at 474.849 kb on + strand at 474.850 kb on - strand at 474.850 kb on - strand at 474.924 kb on + strand at 475.108 kb on + strand at 475.108 kb on + strand at 475.108 kb on + strand at 475.109 kb on - strand at 475.109 kb on - strand at 475.209 kb on - strand, within RR42_RS02145 at 475.210 kb on + strand, within RR42_RS02145 at 475.210 kb on + strand, within RR42_RS02145 at 475.210 kb on + strand, within RR42_RS02145 at 475.211 kb on - strand, within RR42_RS02145 at 475.330 kb on + strand, within RR42_RS02145 at 475.358 kb on - strand, within RR42_RS02145 at 475.358 kb on - strand, within RR42_RS02145 at 475.390 kb on + strand, within RR42_RS02145 at 475.390 kb on + strand, within RR42_RS02145 at 475.391 kb on - strand, within RR42_RS02145 at 475.391 kb on - strand, within RR42_RS02145 at 475.558 kb on + strand, within RR42_RS02145 at 475.558 kb on + strand, within RR42_RS02145 at 475.558 kb on + strand, within RR42_RS02145 at 475.562 kb on + strand, within RR42_RS02145 at 475.563 kb on - strand, within RR42_RS02145 at 475.678 kb on + strand, within RR42_RS02145 at 475.679 kb on - strand, within RR42_RS02145 at 475.679 kb on - strand, within RR42_RS02145 at 475.702 kb on + strand, within RR42_RS02145 at 475.703 kb on - strand, within RR42_RS02145
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 473,631 + RR42_RS02130 0.87 -0.1 473,632 - RR42_RS02130 0.87 +0.1 473,639 + RR42_RS02130 0.87 +0.5 473,640 - RR42_RS02130 0.87 +0.0 473,754 + +0.1 473,784 - +0.5 473,865 - +0.5 473,885 + -0.2 473,885 + +0.3 473,885 + +1.0 473,887 + -0.3 473,887 + -0.5 473,887 + -0.5 473,887 + -1.6 473,888 - +0.5 473,888 - +0.1 473,888 - -1.2 473,888 - -0.9 473,895 - -1.4 474,010 + RR42_RS02135 0.26 +0.2 474,010 + RR42_RS02135 0.26 +0.4 474,011 - RR42_RS02135 0.26 -2.2 474,011 - RR42_RS02135 0.26 -0.8 474,011 - RR42_RS02135 0.26 +0.1 474,055 + RR42_RS02135 0.35 -0.5 474,056 - RR42_RS02135 0.35 +0.1 474,235 + RR42_RS02135 0.72 +0.0 474,236 - RR42_RS02135 0.72 +0.4 474,355 + -0.4 474,355 + +0.0 474,356 - +0.1 474,438 + +0.1 474,438 + -3.1 474,438 + +0.0 474,438 + -0.2 474,438 + +0.5 474,439 - -0.8 474,439 - -1.7 474,439 - -0.3 474,439 - -0.4 474,439 - -0.4 474,443 + +0.8 474,444 - +0.6 474,483 - +0.0 474,611 - RR42_RS02140 0.37 -1.2 474,849 + -1.4 474,850 - +1.1 474,850 - +0.3 474,924 + -0.8 475,108 + -4.5 475,108 + -0.9 475,108 + -3.8 475,109 - -3.9 475,109 - -1.8 475,209 - RR42_RS02145 0.13 -2.6 475,210 + RR42_RS02145 0.13 -4.1 475,210 + RR42_RS02145 0.13 -3.4 475,210 + RR42_RS02145 0.13 -4.0 475,211 - RR42_RS02145 0.13 -1.9 475,330 + RR42_RS02145 0.17 -2.4 475,358 - RR42_RS02145 0.18 -1.8 475,358 - RR42_RS02145 0.18 -3.0 475,390 + RR42_RS02145 0.19 -4.4 475,390 + RR42_RS02145 0.19 -4.7 475,391 - RR42_RS02145 0.19 -3.1 475,391 - RR42_RS02145 0.19 -1.6 475,558 + RR42_RS02145 0.25 -2.0 475,558 + RR42_RS02145 0.25 -3.7 475,558 + RR42_RS02145 0.25 -3.3 475,562 + RR42_RS02145 0.25 -3.7 475,563 - RR42_RS02145 0.25 -2.6 475,678 + RR42_RS02145 0.29 -4.5 475,679 - RR42_RS02145 0.29 -3.3 475,679 - RR42_RS02145 0.29 -2.5 475,702 + RR42_RS02145 0.30 -2.7 475,703 - RR42_RS02145 0.30 -3.3
Or see this region's nucleotide sequence