Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01300 and RR42_RS01305 are separated by 6 nucleotides RR42_RS01305 and RR42_RS01310 overlap by 4 nucleotides RR42_RS01310 and RR42_RS01315 are separated by 92 nucleotides RR42_RS01315 and RR42_RS01320 are separated by 59 nucleotides
RR42_RS01300: RR42_RS01300 - thiamine biosynthesis protein ThiS, at 297,091 to 297,288
_RS01300
RR42_RS01305: RR42_RS01305 - thiazole synthase, at 297,295 to 298,143
_RS01305
RR42_RS01310: RR42_RS01310 - thiamine-phosphate pyrophosphorylase, at 298,140 to 299,273
_RS01310
RR42_RS01315: RR42_RS01315 - GNAT family acetyltransferase, at 299,366 to 299,869
_RS01315
RR42_RS01320: RR42_RS01320 - ATPase AAA, at 299,929 to 300,705
_RS01320
Position (kb) 298
299
300 Strain fitness (log2 ratio) -2
-1
0
1 at 299.401 kb on - strand at 299.401 kb on - strand at 299.439 kb on + strand, within RR42_RS01315 at 299.440 kb on - strand, within RR42_RS01315 at 299.440 kb on - strand, within RR42_RS01315 at 300.037 kb on + strand, within RR42_RS01320 at 300.124 kb on + strand, within RR42_RS01320 at 300.124 kb on + strand, within RR42_RS01320 at 300.125 kb on - strand, within RR42_RS01320 at 300.193 kb on + strand, within RR42_RS01320 at 300.193 kb on + strand, within RR42_RS01320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 299,401 - +0.3 299,401 - +1.4 299,439 + RR42_RS01315 0.14 -0.9 299,440 - RR42_RS01315 0.15 -0.0 299,440 - RR42_RS01315 0.15 -1.9 300,037 + RR42_RS01320 0.14 -0.7 300,124 + RR42_RS01320 0.25 -0.7 300,124 + RR42_RS01320 0.25 -0.5 300,125 - RR42_RS01320 0.25 +0.8 300,193 + RR42_RS01320 0.34 -0.4 300,193 + RR42_RS01320 0.34 -0.9
Or see this region's nucleotide sequence