Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00405 and RR42_RS00410 are separated by 121 nucleotides RR42_RS00410 and RR42_RS00415 are separated by 339 nucleotides RR42_RS00415 and RR42_RS00420 are separated by 128 nucleotides RR42_RS00420 and RR42_RS00425 are separated by 126 nucleotides
RR42_RS00405: RR42_RS00405 - glutamate carboxypeptidase, at 108,369 to 109,688
_RS00405
RR42_RS00410: RR42_RS00410 - transcriptional regulator, at 109,810 to 110,286
_RS00410
RR42_RS00415: RR42_RS00415 - ABC transporter permease, at 110,626 to 111,465
_RS00415
RR42_RS00420: RR42_RS00420 - hypothetical protein, at 111,594 to 111,884
_RS00420
RR42_RS00425: RR42_RS00425 - ABC transporter substrate-binding protein, at 112,011 to 113,024
_RS00425
Position (kb) 110
111
112 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 109.674 kb on - strand at 109.674 kb on - strand at 109.736 kb on + strand at 109.736 kb on + strand at 109.736 kb on + strand at 109.737 kb on - strand at 109.737 kb on - strand at 109.737 kb on - strand at 109.921 kb on + strand, within RR42_RS00410 at 109.921 kb on + strand, within RR42_RS00410 at 109.922 kb on - strand, within RR42_RS00410 at 110.105 kb on + strand, within RR42_RS00410 at 110.106 kb on - strand, within RR42_RS00410 at 110.200 kb on - strand, within RR42_RS00410 at 110.200 kb on - strand, within RR42_RS00410 at 110.286 kb on + strand at 110.446 kb on + strand at 110.609 kb on + strand at 110.610 kb on - strand at 110.665 kb on + strand at 110.666 kb on - strand at 110.666 kb on - strand at 110.666 kb on - strand at 110.818 kb on - strand, within RR42_RS00415 at 110.818 kb on - strand, within RR42_RS00415 at 111.227 kb on - strand, within RR42_RS00415 at 111.227 kb on - strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.362 kb on + strand, within RR42_RS00415 at 111.363 kb on - strand, within RR42_RS00415 at 111.496 kb on - strand at 111.616 kb on + strand at 111.616 kb on + strand at 111.796 kb on + strand, within RR42_RS00420 at 111.797 kb on - strand, within RR42_RS00420 at 111.797 kb on - strand, within RR42_RS00420 at 111.807 kb on + strand, within RR42_RS00420 at 111.807 kb on + strand, within RR42_RS00420 at 111.808 kb on - strand, within RR42_RS00420 at 111.883 kb on - strand at 112.129 kb on - strand, within RR42_RS00425 at 112.374 kb on + strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 109,674 - -0.4 109,674 - +0.3 109,736 + -1.9 109,736 + +0.5 109,736 + +0.4 109,737 - +0.1 109,737 - -0.8 109,737 - +0.8 109,921 + RR42_RS00410 0.23 -0.6 109,921 + RR42_RS00410 0.23 -0.3 109,922 - RR42_RS00410 0.23 -1.0 110,105 + RR42_RS00410 0.62 -1.2 110,106 - RR42_RS00410 0.62 -1.0 110,200 - RR42_RS00410 0.82 +0.4 110,200 - RR42_RS00410 0.82 +0.0 110,286 + -1.6 110,446 + +0.2 110,609 + -0.8 110,610 - -1.8 110,665 + +0.3 110,666 - -0.5 110,666 - +0.4 110,666 - -0.8 110,818 - RR42_RS00415 0.23 -1.3 110,818 - RR42_RS00415 0.23 +1.2 111,227 - RR42_RS00415 0.72 -1.9 111,227 - RR42_RS00415 0.72 -1.5 111,262 + RR42_RS00415 0.76 -0.9 111,262 + RR42_RS00415 0.76 -0.0 111,262 + RR42_RS00415 0.76 -2.9 111,263 - RR42_RS00415 0.76 +0.7 111,263 - RR42_RS00415 0.76 -0.9 111,263 - RR42_RS00415 0.76 -0.6 111,362 + RR42_RS00415 0.88 -0.1 111,363 - RR42_RS00415 0.88 -0.4 111,496 - -0.8 111,616 + -0.4 111,616 + +0.8 111,796 + RR42_RS00420 0.69 -0.4 111,797 - RR42_RS00420 0.70 -1.3 111,797 - RR42_RS00420 0.70 -0.4 111,807 + RR42_RS00420 0.73 +1.1 111,807 + RR42_RS00420 0.73 +0.4 111,808 - RR42_RS00420 0.74 +0.3 111,883 - -0.8 112,129 - RR42_RS00425 0.12 -0.1 112,374 + RR42_RS00425 0.36 -0.4 112,375 - RR42_RS00425 0.36 -1.7 112,375 - RR42_RS00425 0.36 +0.1 112,375 - RR42_RS00425 0.36 +0.3 112,375 - RR42_RS00425 0.36 +1.1
Or see this region's nucleotide sequence