Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_849

Experiment: dimethyl sulfone 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_847 and Pf6N2E2_848 overlap by 8 nucleotidesPf6N2E2_848 and Pf6N2E2_849 overlap by 19 nucleotidesPf6N2E2_849 and Pf6N2E2_850 are separated by 282 nucleotidesPf6N2E2_850 and Pf6N2E2_851 are separated by 96 nucleotides Pf6N2E2_847 - Phosphoribosyl transferase domain protein, at 992,337 to 993,041 _847 Pf6N2E2_848 - hypothetical protein, at 993,034 to 993,729 _848 Pf6N2E2_849 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 993,711 to 995,078 _849 Pf6N2E2_850 - hypothetical protein, at 995,361 to 995,474 _850 Pf6N2E2_851 - Osmotically inducible protein Y precursor, at 995,571 to 996,218 _851 Position (kb) 993 994 995 996Strain fitness (log2 ratio) -2 -1 0 1 2at 992.798 kb on - strand, within Pf6N2E2_847at 992.934 kb on + strand, within Pf6N2E2_847at 992.934 kb on + strand, within Pf6N2E2_847at 992.935 kb on - strand, within Pf6N2E2_847at 993.172 kb on + strand, within Pf6N2E2_848at 993.172 kb on + strand, within Pf6N2E2_848at 993.173 kb on - strand, within Pf6N2E2_848at 993.173 kb on - strand, within Pf6N2E2_848at 993.670 kb on + strandat 993.671 kb on - strandat 993.671 kb on - strandat 993.671 kb on - strandat 993.705 kb on - strandat 993.705 kb on - strandat 993.705 kb on - strandat 993.727 kb on + strandat 993.728 kb on - strandat 993.874 kb on + strand, within Pf6N2E2_849at 993.874 kb on + strand, within Pf6N2E2_849at 994.115 kb on - strand, within Pf6N2E2_849at 994.318 kb on + strand, within Pf6N2E2_849at 994.319 kb on - strand, within Pf6N2E2_849at 994.321 kb on + strand, within Pf6N2E2_849at 994.322 kb on - strand, within Pf6N2E2_849at 994.322 kb on - strand, within Pf6N2E2_849at 994.322 kb on - strand, within Pf6N2E2_849at 994.322 kb on - strand, within Pf6N2E2_849at 994.483 kb on + strand, within Pf6N2E2_849at 994.484 kb on - strand, within Pf6N2E2_849at 994.484 kb on - strand, within Pf6N2E2_849at 994.484 kb on - strand, within Pf6N2E2_849at 994.484 kb on - strand, within Pf6N2E2_849at 994.523 kb on - strand, within Pf6N2E2_849at 994.570 kb on + strand, within Pf6N2E2_849at 994.570 kb on + strand, within Pf6N2E2_849at 994.607 kb on - strand, within Pf6N2E2_849at 994.771 kb on + strand, within Pf6N2E2_849at 994.771 kb on + strand, within Pf6N2E2_849at 994.772 kb on - strand, within Pf6N2E2_849at 994.772 kb on - strand, within Pf6N2E2_849at 994.870 kb on - strand, within Pf6N2E2_849at 994.873 kb on + strand, within Pf6N2E2_849at 994.873 kb on + strand, within Pf6N2E2_849at 994.874 kb on - strand, within Pf6N2E2_849at 994.874 kb on - strand, within Pf6N2E2_849at 995.018 kb on + strandat 995.019 kb on - strandat 995.029 kb on + strandat 995.030 kb on - strandat 995.041 kb on + strandat 995.125 kb on + strandat 995.125 kb on + strandat 995.125 kb on + strandat 995.125 kb on + strandat 995.126 kb on - strandat 995.126 kb on - strandat 995.126 kb on - strandat 995.215 kb on + strandat 995.219 kb on + strandat 995.219 kb on + strandat 995.219 kb on + strandat 995.220 kb on - strandat 995.225 kb on + strandat 995.226 kb on - strandat 995.290 kb on - strandat 995.297 kb on + strandat 995.323 kb on - strandat 995.362 kb on + strandat 995.739 kb on - strand, within Pf6N2E2_851at 995.864 kb on - strand, within Pf6N2E2_851at 995.928 kb on + strand, within Pf6N2E2_851at 995.929 kb on - strand, within Pf6N2E2_851at 995.929 kb on - strand, within Pf6N2E2_851at 996.015 kb on + strand, within Pf6N2E2_851at 996.015 kb on + strand, within Pf6N2E2_851at 996.015 kb on + strand, within Pf6N2E2_851at 996.016 kb on - strand, within Pf6N2E2_851at 996.016 kb on - strand, within Pf6N2E2_851at 996.016 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M
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992,798 - Pf6N2E2_847 0.65 +0.9
992,934 + Pf6N2E2_847 0.85 -0.4
992,934 + Pf6N2E2_847 0.85 +0.8
992,935 - Pf6N2E2_847 0.85 +0.5
993,172 + Pf6N2E2_848 0.20 +0.5
993,172 + Pf6N2E2_848 0.20 +0.5
993,173 - Pf6N2E2_848 0.20 +0.1
993,173 - Pf6N2E2_848 0.20 -0.4
993,670 + +0.4
993,671 - +0.1
993,671 - -0.7
993,671 - -1.3
993,705 - -0.3
993,705 - -1.1
993,705 - +0.8
993,727 + -1.1
993,728 - +0.1
993,874 + Pf6N2E2_849 0.12 -1.2
993,874 + Pf6N2E2_849 0.12 -0.4
994,115 - Pf6N2E2_849 0.30 -0.8
994,318 + Pf6N2E2_849 0.44 -0.8
994,319 - Pf6N2E2_849 0.44 -0.4
994,321 + Pf6N2E2_849 0.45 -0.6
994,322 - Pf6N2E2_849 0.45 +0.6
994,322 - Pf6N2E2_849 0.45 -0.6
994,322 - Pf6N2E2_849 0.45 -0.1
994,322 - Pf6N2E2_849 0.45 +0.7
994,483 + Pf6N2E2_849 0.56 -1.0
994,484 - Pf6N2E2_849 0.57 -0.2
994,484 - Pf6N2E2_849 0.57 -1.2
994,484 - Pf6N2E2_849 0.57 +1.4
994,484 - Pf6N2E2_849 0.57 +0.2
994,523 - Pf6N2E2_849 0.59 +0.7
994,570 + Pf6N2E2_849 0.63 +0.2
994,570 + Pf6N2E2_849 0.63 +0.4
994,607 - Pf6N2E2_849 0.65 +0.4
994,771 + Pf6N2E2_849 0.77 +1.2
994,771 + Pf6N2E2_849 0.77 -0.8
994,772 - Pf6N2E2_849 0.78 +0.5
994,772 - Pf6N2E2_849 0.78 +0.7
994,870 - Pf6N2E2_849 0.85 -0.9
994,873 + Pf6N2E2_849 0.85 -0.1
994,873 + Pf6N2E2_849 0.85 +0.1
994,874 - Pf6N2E2_849 0.85 -1.9
994,874 - Pf6N2E2_849 0.85 -2.1
995,018 + +0.5
995,019 - +0.2
995,029 + -0.7
995,030 - +0.4
995,041 + -0.2
995,125 + +0.0
995,125 + -1.2
995,125 + +0.3
995,125 + -0.1
995,126 - +1.4
995,126 - +0.2
995,126 - +0.3
995,215 + +0.5
995,219 + -0.1
995,219 + +0.0
995,219 + -1.2
995,220 - -1.5
995,225 + +0.1
995,226 - -0.6
995,290 - -1.9
995,297 + -0.6
995,323 - +0.9
995,362 + +2.2
995,739 - Pf6N2E2_851 0.26 +0.2
995,864 - Pf6N2E2_851 0.45 -0.9
995,928 + Pf6N2E2_851 0.55 -1.3
995,929 - Pf6N2E2_851 0.55 +0.4
995,929 - Pf6N2E2_851 0.55 +1.9
996,015 + Pf6N2E2_851 0.69 +0.4
996,015 + Pf6N2E2_851 0.69 +1.7
996,015 + Pf6N2E2_851 0.69 -0.8
996,016 - Pf6N2E2_851 0.69 +0.9
996,016 - Pf6N2E2_851 0.69 +0.5
996,016 - +1.4

Or see this region's nucleotide sequence