Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_821 and Pf6N2E2_822 are separated by 341 nucleotides Pf6N2E2_822 and Pf6N2E2_823 are separated by 297 nucleotides
Pf6N2E2_821 - KaiC, at 955,360 to 956,862
_821
Pf6N2E2_822 - TPR domain protein, at 957,204 to 958,262
_822
Pf6N2E2_823 - Outer membrane protein OprN, at 958,560 to 959,981
_823
Position (kb)
957
958
959 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 956.204 kb on + strand, within Pf6N2E2_821 at 956.219 kb on + strand, within Pf6N2E2_821 at 956.255 kb on + strand, within Pf6N2E2_821 at 956.256 kb on - strand, within Pf6N2E2_821 at 956.256 kb on - strand, within Pf6N2E2_821 at 956.256 kb on - strand, within Pf6N2E2_821 at 956.375 kb on + strand, within Pf6N2E2_821 at 956.375 kb on + strand, within Pf6N2E2_821 at 956.375 kb on + strand, within Pf6N2E2_821 at 956.375 kb on + strand, within Pf6N2E2_821 at 956.376 kb on - strand, within Pf6N2E2_821 at 956.534 kb on + strand, within Pf6N2E2_821 at 956.595 kb on + strand, within Pf6N2E2_821 at 956.596 kb on - strand, within Pf6N2E2_821 at 956.675 kb on + strand, within Pf6N2E2_821 at 956.675 kb on + strand, within Pf6N2E2_821 at 956.676 kb on - strand, within Pf6N2E2_821 at 956.676 kb on - strand, within Pf6N2E2_821 at 956.850 kb on + strand at 956.851 kb on - strand at 956.856 kb on + strand at 956.866 kb on + strand at 956.963 kb on + strand at 956.963 kb on + strand at 956.963 kb on + strand at 956.972 kb on - strand at 956.983 kb on + strand at 956.983 kb on + strand at 956.984 kb on - strand at 956.984 kb on - strand at 957.016 kb on - strand at 957.036 kb on + strand at 957.199 kb on + strand at 957.199 kb on + strand at 957.206 kb on - strand at 957.301 kb on + strand at 957.301 kb on + strand at 957.301 kb on + strand at 957.302 kb on - strand at 957.302 kb on - strand at 957.419 kb on - strand, within Pf6N2E2_822 at 957.478 kb on + strand, within Pf6N2E2_822 at 957.478 kb on + strand, within Pf6N2E2_822 at 957.479 kb on - strand, within Pf6N2E2_822 at 957.530 kb on - strand, within Pf6N2E2_822 at 957.570 kb on + strand, within Pf6N2E2_822 at 957.606 kb on + strand, within Pf6N2E2_822 at 957.606 kb on + strand, within Pf6N2E2_822 at 957.607 kb on - strand, within Pf6N2E2_822 at 958.016 kb on - strand, within Pf6N2E2_822 at 958.084 kb on + strand, within Pf6N2E2_822 at 958.084 kb on + strand, within Pf6N2E2_822 at 958.085 kb on - strand, within Pf6N2E2_822 at 958.142 kb on - strand, within Pf6N2E2_822 at 958.191 kb on + strand at 958.240 kb on + strand at 958.609 kb on + strand at 958.609 kb on + strand at 958.609 kb on + strand at 958.609 kb on + strand at 958.609 kb on + strand at 958.609 kb on + strand at 958.851 kb on + strand, within Pf6N2E2_823 at 958.851 kb on + strand, within Pf6N2E2_823 at 958.851 kb on + strand, within Pf6N2E2_823 at 958.852 kb on - strand, within Pf6N2E2_823 at 958.852 kb on - strand, within Pf6N2E2_823 at 958.852 kb on - strand, within Pf6N2E2_823 at 958.864 kb on + strand, within Pf6N2E2_823 at 958.864 kb on + strand, within Pf6N2E2_823 at 958.864 kb on + strand, within Pf6N2E2_823 at 958.864 kb on + strand, within Pf6N2E2_823 at 958.865 kb on - strand, within Pf6N2E2_823 at 958.946 kb on + strand, within Pf6N2E2_823 at 958.947 kb on - strand, within Pf6N2E2_823 at 958.947 kb on - strand at 958.947 kb on - strand, within Pf6N2E2_823 at 958.947 kb on - strand, within Pf6N2E2_823 at 958.947 kb on - strand, within Pf6N2E2_823 at 958.947 kb on - strand, within Pf6N2E2_823
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 956,204 + Pf6N2E2_821 0.56 -2.0 956,219 + Pf6N2E2_821 0.57 -2.1 956,255 + Pf6N2E2_821 0.60 -1.0 956,256 - Pf6N2E2_821 0.60 +0.5 956,256 - Pf6N2E2_821 0.60 -0.1 956,256 - Pf6N2E2_821 0.60 -0.2 956,375 + Pf6N2E2_821 0.68 -0.9 956,375 + Pf6N2E2_821 0.68 -1.0 956,375 + Pf6N2E2_821 0.68 -1.6 956,375 + Pf6N2E2_821 0.68 +0.6 956,376 - Pf6N2E2_821 0.68 -0.5 956,534 + Pf6N2E2_821 0.78 +2.7 956,595 + Pf6N2E2_821 0.82 -0.6 956,596 - Pf6N2E2_821 0.82 +0.4 956,675 + Pf6N2E2_821 0.87 +0.4 956,675 + Pf6N2E2_821 0.87 -1.2 956,676 - Pf6N2E2_821 0.88 +0.0 956,676 - Pf6N2E2_821 0.88 -1.0 956,850 + -1.4 956,851 - +0.1 956,856 + +0.5 956,866 + -0.8 956,963 + +1.7 956,963 + +0.1 956,963 + -0.2 956,972 - +0.2 956,983 + +0.5 956,983 + -0.1 956,984 - +0.5 956,984 - -0.3 957,016 - +0.4 957,036 + -0.9 957,199 + -0.3 957,199 + +0.8 957,206 - +0.4 957,301 + +1.4 957,301 + +0.8 957,301 + -0.6 957,302 - +0.7 957,302 - +1.2 957,419 - Pf6N2E2_822 0.20 +2.2 957,478 + Pf6N2E2_822 0.26 +1.4 957,478 + Pf6N2E2_822 0.26 +1.3 957,479 - Pf6N2E2_822 0.26 +0.5 957,530 - Pf6N2E2_822 0.31 +0.8 957,570 + Pf6N2E2_822 0.35 -0.3 957,606 + Pf6N2E2_822 0.38 +0.0 957,606 + Pf6N2E2_822 0.38 +0.9 957,607 - Pf6N2E2_822 0.38 +0.2 958,016 - Pf6N2E2_822 0.77 +1.4 958,084 + Pf6N2E2_822 0.83 +0.1 958,084 + Pf6N2E2_822 0.83 +1.4 958,085 - Pf6N2E2_822 0.83 +0.5 958,142 - Pf6N2E2_822 0.89 +1.3 958,191 + +0.8 958,240 + +1.0 958,609 + -0.5 958,609 + +0.4 958,609 + +1.1 958,609 + -0.5 958,609 + -1.6 958,609 + +0.9 958,851 + Pf6N2E2_823 0.20 +0.2 958,851 + Pf6N2E2_823 0.20 +0.8 958,851 + Pf6N2E2_823 0.20 +0.9 958,852 - Pf6N2E2_823 0.21 -0.8 958,852 - Pf6N2E2_823 0.21 +0.4 958,852 - Pf6N2E2_823 0.21 +0.1 958,864 + Pf6N2E2_823 0.21 -0.4 958,864 + Pf6N2E2_823 0.21 +0.1 958,864 + Pf6N2E2_823 0.21 -0.8 958,864 + Pf6N2E2_823 0.21 -0.9 958,865 - Pf6N2E2_823 0.21 +0.5 958,946 + Pf6N2E2_823 0.27 -1.1 958,947 - Pf6N2E2_823 0.27 +1.8 958,947 - +1.4 958,947 - Pf6N2E2_823 0.27 +0.4 958,947 - Pf6N2E2_823 0.27 +2.4 958,947 - Pf6N2E2_823 0.27 -0.2 958,947 - Pf6N2E2_823 0.27 +0.8
Or see this region's nucleotide sequence