Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_812 and Pf6N2E2_813 are separated by 77 nucleotides Pf6N2E2_813 and Pf6N2E2_814 are separated by 212 nucleotides Pf6N2E2_814 and Pf6N2E2_815 overlap by 30 nucleotides
Pf6N2E2_812 - Glycosyltransferase, at 944,886 to 946,178
_812
Pf6N2E2_813 - Response regulator, at 946,256 to 946,561
_813
Pf6N2E2_814 - hypothetical protein, at 946,774 to 946,917
_814
Pf6N2E2_815 - Signal transduction histidine kinase, at 946,888 to 950,370
_815
Position (kb) 946
947 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 945.369 kb on - strand, within Pf6N2E2_812 at 945.397 kb on + strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.407 kb on - strand, within Pf6N2E2_812 at 946.138 kb on + strand at 946.138 kb on + strand at 946.138 kb on + strand at 946.138 kb on + strand at 946.139 kb on - strand at 946.342 kb on + strand, within Pf6N2E2_813 at 946.343 kb on - strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.527 kb on + strand, within Pf6N2E2_813 at 946.627 kb on + strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.628 kb on - strand at 946.690 kb on + strand at 946.690 kb on + strand at 946.690 kb on + strand at 946.691 kb on - strand at 946.724 kb on - strand at 946.737 kb on + strand at 946.737 kb on + strand at 946.738 kb on - strand at 946.739 kb on + strand at 946.740 kb on - strand at 946.742 kb on + strand at 946.775 kb on + strand at 946.776 kb on - strand at 946.782 kb on + strand at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.819 kb on + strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.820 kb on - strand, within Pf6N2E2_814 at 946.944 kb on + strand at 946.944 kb on + strand at 946.944 kb on + strand at 947.005 kb on + strand at 947.005 kb on + strand at 947.005 kb on + strand at 947.006 kb on - strand at 947.146 kb on + strand at 947.184 kb on + strand at 947.185 kb on - strand at 947.185 kb on - strand at 947.240 kb on + strand, within Pf6N2E2_815 at 947.240 kb on + strand, within Pf6N2E2_815 at 947.240 kb on + strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.241 kb on - strand, within Pf6N2E2_815 at 947.270 kb on - strand, within Pf6N2E2_815 at 947.270 kb on - strand, within Pf6N2E2_815 at 947.458 kb on + strand, within Pf6N2E2_815 at 947.458 kb on + strand, within Pf6N2E2_815 at 947.459 kb on - strand, within Pf6N2E2_815 at 947.497 kb on + strand, within Pf6N2E2_815 at 947.498 kb on - strand, within Pf6N2E2_815 at 947.498 kb on - strand, within Pf6N2E2_815 at 947.536 kb on + strand, within Pf6N2E2_815 at 947.536 kb on + strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815 at 947.537 kb on - strand, within Pf6N2E2_815
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 945,369 - Pf6N2E2_812 0.37 -0.9 945,397 + Pf6N2E2_812 0.40 +0.3 945,398 - Pf6N2E2_812 0.40 +0.1 945,398 - Pf6N2E2_812 0.40 +2.0 945,398 - Pf6N2E2_812 0.40 +0.5 945,407 - Pf6N2E2_812 0.40 +0.3 946,138 + -1.1 946,138 + +0.4 946,138 + -0.3 946,138 + -0.1 946,139 - +1.8 946,342 + Pf6N2E2_813 0.28 +1.9 946,343 - Pf6N2E2_813 0.28 +3.5 946,527 + Pf6N2E2_813 0.89 +3.2 946,527 + Pf6N2E2_813 0.89 +2.9 946,527 + Pf6N2E2_813 0.89 +2.0 946,527 + Pf6N2E2_813 0.89 +4.1 946,527 + Pf6N2E2_813 0.89 +1.9 946,627 + +0.9 946,628 - +2.7 946,628 - +3.5 946,628 - +3.1 946,628 - +2.4 946,628 - +2.9 946,628 - +1.4 946,628 - +2.2 946,690 + +1.0 946,690 + +0.2 946,690 + +1.7 946,691 - +1.1 946,724 - -0.6 946,737 + +1.3 946,737 + +1.4 946,738 - +0.6 946,739 + +2.4 946,740 - -0.2 946,742 + +1.5 946,775 + +1.2 946,776 - +0.5 946,782 + -0.7 946,819 + Pf6N2E2_814 0.31 +0.9 946,819 + Pf6N2E2_814 0.31 +0.1 946,819 + Pf6N2E2_814 0.31 +0.0 946,819 + Pf6N2E2_814 0.31 -1.3 946,820 - Pf6N2E2_814 0.32 +0.4 946,820 - Pf6N2E2_814 0.32 -1.7 946,820 - Pf6N2E2_814 0.32 +0.3 946,820 - Pf6N2E2_814 0.32 -0.6 946,944 + +1.6 946,944 + +1.8 946,944 + +0.7 947,005 + +0.1 947,005 + +3.2 947,005 + +1.4 947,006 - +3.0 947,146 + +0.7 947,184 + +1.6 947,185 - +0.8 947,185 - +0.9 947,240 + Pf6N2E2_815 0.10 +0.1 947,240 + Pf6N2E2_815 0.10 +1.6 947,240 + Pf6N2E2_815 0.10 -0.5 947,241 - Pf6N2E2_815 0.10 +1.0 947,241 - Pf6N2E2_815 0.10 -0.5 947,241 - Pf6N2E2_815 0.10 +0.6 947,241 - Pf6N2E2_815 0.10 +1.0 947,270 - Pf6N2E2_815 0.11 +0.7 947,270 - Pf6N2E2_815 0.11 +1.4 947,458 + Pf6N2E2_815 0.16 +1.0 947,458 + Pf6N2E2_815 0.16 +0.9 947,459 - Pf6N2E2_815 0.16 -0.5 947,497 + Pf6N2E2_815 0.17 +1.8 947,498 - Pf6N2E2_815 0.18 +1.6 947,498 - Pf6N2E2_815 0.18 +1.0 947,536 + Pf6N2E2_815 0.19 +1.4 947,536 + Pf6N2E2_815 0.19 +1.8 947,537 - Pf6N2E2_815 0.19 +2.2 947,537 - Pf6N2E2_815 0.19 +0.4 947,537 - Pf6N2E2_815 0.19 +0.4 947,537 - Pf6N2E2_815 0.19 +1.4
Or see this region's nucleotide sequence