Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_500 and Pf6N2E2_501 are separated by 84 nucleotides Pf6N2E2_501 and Pf6N2E2_502 are separated by 239 nucleotides Pf6N2E2_502 and Pf6N2E2_503 are separated by 185 nucleotides
Pf6N2E2_500 - Beta-ureidopropionase (EC 3.5.1.6), at 569,178 to 570,461
_500
Pf6N2E2_501 - Sensory box transcriptional regulator, LuxR family, at 570,546 to 572,030
_501
Pf6N2E2_502 - hypothetical protein, at 572,270 to 572,515
_502
Pf6N2E2_503 - sensor histidine kinase, at 572,701 to 574,086
_503
Position (kb) 570
571
572
573 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 569.760 kb on + strand, within Pf6N2E2_500 at 569.761 kb on - strand, within Pf6N2E2_500 at 569.909 kb on + strand, within Pf6N2E2_500 at 569.910 kb on - strand, within Pf6N2E2_500 at 570.190 kb on - strand, within Pf6N2E2_500 at 570.476 kb on - strand at 570.497 kb on - strand at 570.526 kb on - strand at 570.526 kb on - strand at 570.526 kb on - strand at 570.526 kb on - strand at 570.726 kb on + strand, within Pf6N2E2_501 at 570.726 kb on + strand, within Pf6N2E2_501 at 570.726 kb on + strand, within Pf6N2E2_501 at 570.727 kb on - strand, within Pf6N2E2_501 at 570.727 kb on - strand, within Pf6N2E2_501 at 570.783 kb on + strand, within Pf6N2E2_501 at 570.784 kb on - strand, within Pf6N2E2_501 at 570.784 kb on - strand, within Pf6N2E2_501 at 571.038 kb on + strand, within Pf6N2E2_501 at 571.038 kb on + strand, within Pf6N2E2_501 at 571.039 kb on - strand, within Pf6N2E2_501 at 571.111 kb on - strand, within Pf6N2E2_501 at 571.647 kb on + strand, within Pf6N2E2_501 at 571.647 kb on + strand, within Pf6N2E2_501 at 571.647 kb on + strand, within Pf6N2E2_501 at 571.648 kb on - strand, within Pf6N2E2_501 at 571.648 kb on - strand, within Pf6N2E2_501 at 571.648 kb on - strand, within Pf6N2E2_501 at 571.908 kb on - strand at 571.929 kb on - strand at 571.935 kb on + strand at 571.996 kb on + strand at 572.177 kb on + strand at 572.178 kb on - strand at 572.185 kb on + strand at 572.186 kb on - strand at 572.186 kb on - strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.206 kb on - strand at 572.364 kb on + strand, within Pf6N2E2_502 at 572.372 kb on + strand, within Pf6N2E2_502 at 572.373 kb on - strand, within Pf6N2E2_502 at 572.373 kb on - strand, within Pf6N2E2_502 at 572.474 kb on + strand, within Pf6N2E2_502 at 572.489 kb on - strand, within Pf6N2E2_502 at 572.561 kb on - strand at 572.561 kb on - strand at 572.561 kb on - strand at 572.637 kb on + strand at 572.703 kb on - strand at 572.761 kb on + strand at 572.761 kb on + strand at 572.761 kb on + strand at 572.855 kb on + strand, within Pf6N2E2_503 at 572.856 kb on - strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.026 kb on - strand, within Pf6N2E2_503
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 569,760 + Pf6N2E2_500 0.45 +1.1 569,761 - Pf6N2E2_500 0.45 -0.4 569,909 + Pf6N2E2_500 0.57 +0.2 569,910 - Pf6N2E2_500 0.57 -0.9 570,190 - Pf6N2E2_500 0.79 -0.1 570,476 - -0.1 570,497 - +0.5 570,526 - +0.5 570,526 - +0.1 570,526 - +0.2 570,526 - -0.7 570,726 + Pf6N2E2_501 0.12 -0.5 570,726 + Pf6N2E2_501 0.12 +1.0 570,726 + Pf6N2E2_501 0.12 +2.3 570,727 - Pf6N2E2_501 0.12 -1.7 570,727 - Pf6N2E2_501 0.12 -1.7 570,783 + Pf6N2E2_501 0.16 -0.6 570,784 - Pf6N2E2_501 0.16 +0.1 570,784 - Pf6N2E2_501 0.16 -2.0 571,038 + Pf6N2E2_501 0.33 +1.4 571,038 + Pf6N2E2_501 0.33 -0.8 571,039 - Pf6N2E2_501 0.33 -1.1 571,111 - Pf6N2E2_501 0.38 +0.0 571,647 + Pf6N2E2_501 0.74 +0.2 571,647 + Pf6N2E2_501 0.74 +0.4 571,647 + Pf6N2E2_501 0.74 -2.8 571,648 - Pf6N2E2_501 0.74 -1.3 571,648 - Pf6N2E2_501 0.74 -0.1 571,648 - Pf6N2E2_501 0.74 -0.1 571,908 - +1.0 571,929 - -1.1 571,935 + +0.7 571,996 + +0.4 572,177 + +0.7 572,178 - -0.6 572,185 + -0.3 572,186 - +0.9 572,186 - +1.2 572,205 + -0.9 572,205 + +0.4 572,205 + -1.3 572,206 - -0.1 572,364 + Pf6N2E2_502 0.38 +0.2 572,372 + Pf6N2E2_502 0.41 -1.3 572,373 - Pf6N2E2_502 0.42 +0.7 572,373 - Pf6N2E2_502 0.42 -0.9 572,474 + Pf6N2E2_502 0.83 -1.3 572,489 - Pf6N2E2_502 0.89 +0.1 572,561 - -0.7 572,561 - -0.3 572,561 - +0.0 572,637 + -1.1 572,703 - -0.4 572,761 + -0.1 572,761 + -0.1 572,761 + +0.6 572,855 + Pf6N2E2_503 0.11 +0.5 572,856 - Pf6N2E2_503 0.11 +0.3 573,025 + Pf6N2E2_503 0.23 -0.4 573,025 + Pf6N2E2_503 0.23 +0.1 573,025 + Pf6N2E2_503 0.23 +0.9 573,026 - Pf6N2E2_503 0.23 -1.0
Or see this region's nucleotide sequence