Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3013 and Pf6N2E2_3014 are separated by 9 nucleotides Pf6N2E2_3014 and Pf6N2E2_3015 overlap by 8 nucleotides Pf6N2E2_3015 and Pf6N2E2_3016 overlap by 1 nucleotides Pf6N2E2_3016 and Pf6N2E2_3017 are separated by 6 nucleotides Pf6N2E2_3017 and Pf6N2E2_3018 overlap by 4 nucleotides Pf6N2E2_3018 and Pf6N2E2_3019 are separated by 152 nucleotides
Pf6N2E2_3013 - FIG032563: Phage tail protein D, at 3,501,110 to 3,502,126
_3013
Pf6N2E2_3014 - P2-like prophage tail protein X, at 3,502,136 to 3,502,348
_3014
Pf6N2E2_3015 - hypothetical protein, at 3,502,341 to 3,502,724
_3015
Pf6N2E2_3016 - Phage tail length tape-measure protein, at 3,502,724 to 3,504,901
_3016
Pf6N2E2_3017 - hypothetical protein, at 3,504,908 to 3,505,033
_3017
Pf6N2E2_3018 - FIG00955390: hypothetical protein, at 3,505,030 to 3,505,596
_3018
Pf6N2E2_3019 - FIG00954559: hypothetical protein, at 3,505,749 to 3,506,255
_3019
Position (kb)
3502
3503
3504
3505 Strain fitness (log2 ratio)
-1
0
1
2 at 3502.217 kb on + strand, within Pf6N2E2_3014 at 3502.217 kb on + strand, within Pf6N2E2_3014 at 3502.299 kb on + strand, within Pf6N2E2_3014 at 3502.543 kb on + strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.729 kb on + strand at 3502.729 kb on + strand at 3502.730 kb on - strand at 3502.730 kb on - strand at 3502.866 kb on + strand at 3502.866 kb on + strand at 3502.867 kb on - strand at 3503.168 kb on + strand, within Pf6N2E2_3016 at 3503.207 kb on + strand, within Pf6N2E2_3016 at 3503.297 kb on - strand, within Pf6N2E2_3016 at 3503.297 kb on - strand, within Pf6N2E2_3016 at 3503.297 kb on - strand, within Pf6N2E2_3016 at 3503.306 kb on + strand, within Pf6N2E2_3016 at 3503.477 kb on + strand, within Pf6N2E2_3016 at 3503.548 kb on + strand, within Pf6N2E2_3016 at 3504.033 kb on - strand, within Pf6N2E2_3016 at 3504.608 kb on + strand, within Pf6N2E2_3016 at 3504.608 kb on + strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.626 kb on - strand, within Pf6N2E2_3016 at 3504.827 kb on - strand at 3504.835 kb on + strand at 3504.836 kb on - strand at 3504.836 kb on - strand at 3504.874 kb on - strand at 3504.874 kb on - strand at 3504.885 kb on + strand at 3504.886 kb on - strand at 3504.890 kb on - strand at 3504.890 kb on - strand at 3504.890 kb on - strand at 3504.909 kb on + strand at 3504.909 kb on + strand at 3504.910 kb on - strand at 3504.998 kb on + strand, within Pf6N2E2_3017 at 3504.999 kb on - strand, within Pf6N2E2_3017 at 3504.999 kb on - strand, within Pf6N2E2_3017 at 3505.006 kb on - strand, within Pf6N2E2_3017 at 3505.073 kb on - strand at 3505.291 kb on + strand, within Pf6N2E2_3018 at 3505.291 kb on + strand, within Pf6N2E2_3018 at 3505.292 kb on - strand, within Pf6N2E2_3018 at 3505.292 kb on - strand, within Pf6N2E2_3018 at 3505.361 kb on + strand, within Pf6N2E2_3018 at 3505.361 kb on + strand, within Pf6N2E2_3018 at 3505.361 kb on + strand, within Pf6N2E2_3018 at 3505.362 kb on - strand, within Pf6N2E2_3018 at 3505.362 kb on - strand, within Pf6N2E2_3018 at 3505.380 kb on - strand, within Pf6N2E2_3018 at 3505.502 kb on + strand, within Pf6N2E2_3018 at 3505.613 kb on + strand at 3505.614 kb on - strand at 3505.614 kb on - strand at 3505.864 kb on + strand, within Pf6N2E2_3019 at 3505.864 kb on + strand, within Pf6N2E2_3019 at 3505.865 kb on - strand, within Pf6N2E2_3019 at 3505.865 kb on - strand, within Pf6N2E2_3019 at 3505.865 kb on - strand, within Pf6N2E2_3019
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 3,502,217 + Pf6N2E2_3014 0.38 -0.6 3,502,217 + Pf6N2E2_3014 0.38 +0.0 3,502,299 + Pf6N2E2_3014 0.77 +0.3 3,502,543 + Pf6N2E2_3015 0.53 +1.3 3,502,544 - Pf6N2E2_3015 0.53 +0.7 3,502,544 - Pf6N2E2_3015 0.53 +1.1 3,502,544 - Pf6N2E2_3015 0.53 -0.5 3,502,729 + +0.6 3,502,729 + -0.2 3,502,730 - -1.3 3,502,730 - -0.6 3,502,866 + +0.1 3,502,866 + -0.3 3,502,867 - -0.7 3,503,168 + Pf6N2E2_3016 0.20 -0.5 3,503,207 + Pf6N2E2_3016 0.22 -0.9 3,503,297 - Pf6N2E2_3016 0.26 -0.0 3,503,297 - Pf6N2E2_3016 0.26 -0.7 3,503,297 - Pf6N2E2_3016 0.26 -0.6 3,503,306 + Pf6N2E2_3016 0.27 -0.7 3,503,477 + Pf6N2E2_3016 0.35 +0.1 3,503,548 + Pf6N2E2_3016 0.38 -0.8 3,504,033 - Pf6N2E2_3016 0.60 -1.6 3,504,608 + Pf6N2E2_3016 0.87 +0.6 3,504,608 + Pf6N2E2_3016 0.87 -0.1 3,504,609 - Pf6N2E2_3016 0.87 +0.2 3,504,609 - Pf6N2E2_3016 0.87 +0.0 3,504,609 - Pf6N2E2_3016 0.87 +0.8 3,504,609 - Pf6N2E2_3016 0.87 -0.2 3,504,626 - Pf6N2E2_3016 0.87 -0.4 3,504,827 - +0.5 3,504,835 + -1.5 3,504,836 - +0.5 3,504,836 - +0.7 3,504,874 - +0.5 3,504,874 - +0.6 3,504,885 + +1.0 3,504,886 - +0.2 3,504,890 - -0.6 3,504,890 - +2.4 3,504,890 - -0.9 3,504,909 + -1.3 3,504,909 + -0.3 3,504,910 - +0.7 3,504,998 + Pf6N2E2_3017 0.71 +0.0 3,504,999 - Pf6N2E2_3017 0.72 +1.0 3,504,999 - Pf6N2E2_3017 0.72 -0.4 3,505,006 - Pf6N2E2_3017 0.78 +0.3 3,505,073 - -0.6 3,505,291 + Pf6N2E2_3018 0.46 +1.6 3,505,291 + Pf6N2E2_3018 0.46 -0.0 3,505,292 - Pf6N2E2_3018 0.46 -1.0 3,505,292 - Pf6N2E2_3018 0.46 -0.2 3,505,361 + Pf6N2E2_3018 0.58 +0.6 3,505,361 + Pf6N2E2_3018 0.58 +0.3 3,505,361 + Pf6N2E2_3018 0.58 +0.1 3,505,362 - Pf6N2E2_3018 0.59 -0.0 3,505,362 - Pf6N2E2_3018 0.59 +1.2 3,505,380 - Pf6N2E2_3018 0.62 -0.5 3,505,502 + Pf6N2E2_3018 0.83 +0.2 3,505,613 + +0.8 3,505,614 - -0.2 3,505,614 - +0.9 3,505,864 + Pf6N2E2_3019 0.23 +0.0 3,505,864 + Pf6N2E2_3019 0.23 +0.4 3,505,865 - Pf6N2E2_3019 0.23 -0.9 3,505,865 - Pf6N2E2_3019 0.23 -0.4 3,505,865 - Pf6N2E2_3019 0.23 +1.2
Or see this region's nucleotide sequence