Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_134

Experiment: dimethyl sulfone 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_132 and Pf6N2E2_133 are separated by 42 nucleotidesPf6N2E2_133 and Pf6N2E2_134 are separated by 342 nucleotidesPf6N2E2_134 and Pf6N2E2_135 are separated by 88 nucleotides Pf6N2E2_132 - hcp, at 181,377 to 181,658 _132 Pf6N2E2_133 - hypothetical protein, at 181,701 to 181,850 _133 Pf6N2E2_134 - FIG00960493: hypothetical protein, at 182,193 to 183,569 _134 Pf6N2E2_135 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 183,658 to 184,878 _135 Position (kb) 182 183 184Strain fitness (log2 ratio) -2 -1 0 1 2at 181.205 kb on - strandat 181.221 kb on + strandat 181.221 kb on + strandat 181.221 kb on + strandat 181.224 kb on + strandat 181.224 kb on + strandat 181.224 kb on + strandat 181.224 kb on + strandat 181.224 kb on + strandat 181.224 kb on + strandat 181.224 kb on + strandat 181.224 kb on + strandat 181.225 kb on - strandat 181.225 kb on - strandat 181.225 kb on - strandat 181.225 kb on - strandat 181.255 kb on + strandat 181.256 kb on - strandat 181.312 kb on - strandat 181.321 kb on + strandat 181.321 kb on + strandat 181.428 kb on + strand, within Pf6N2E2_132at 181.428 kb on + strand, within Pf6N2E2_132at 181.429 kb on - strand, within Pf6N2E2_132at 181.429 kb on - strand, within Pf6N2E2_132at 181.429 kb on - strand, within Pf6N2E2_132at 181.519 kb on + strand, within Pf6N2E2_132at 181.519 kb on + strand, within Pf6N2E2_132at 181.564 kb on + strand, within Pf6N2E2_132at 181.565 kb on - strand, within Pf6N2E2_132at 181.565 kb on - strand, within Pf6N2E2_132at 181.567 kb on + strand, within Pf6N2E2_132at 181.567 kb on + strand, within Pf6N2E2_132at 181.567 kb on + strand, within Pf6N2E2_132at 181.568 kb on - strand, within Pf6N2E2_132at 181.568 kb on - strand, within Pf6N2E2_132at 181.568 kb on - strand, within Pf6N2E2_132at 181.683 kb on - strandat 181.692 kb on + strandat 181.693 kb on - strandat 181.693 kb on - strandat 181.705 kb on - strandat 181.705 kb on - strandat 181.790 kb on + strand, within Pf6N2E2_133at 181.811 kb on + strand, within Pf6N2E2_133at 181.829 kb on + strand, within Pf6N2E2_133at 181.851 kb on + strandat 181.851 kb on + strandat 181.851 kb on + strandat 181.851 kb on + strandat 181.852 kb on - strandat 181.852 kb on - strandat 181.852 kb on - strandat 181.852 kb on - strandat 181.852 kb on - strandat 181.879 kb on + strandat 181.879 kb on + strandat 181.879 kb on + strandat 181.880 kb on - strandat 182.188 kb on + strandat 182.320 kb on + strandat 182.320 kb on + strandat 182.392 kb on + strand, within Pf6N2E2_134at 182.392 kb on + strand, within Pf6N2E2_134at 182.393 kb on - strand, within Pf6N2E2_134at 182.393 kb on - strand, within Pf6N2E2_134at 182.416 kb on + strand, within Pf6N2E2_134at 182.416 kb on + strand, within Pf6N2E2_134at 182.417 kb on - strand, within Pf6N2E2_134at 182.506 kb on + strand, within Pf6N2E2_134at 182.506 kb on + strand, within Pf6N2E2_134at 182.506 kb on + strand, within Pf6N2E2_134at 182.506 kb on + strand, within Pf6N2E2_134at 182.581 kb on + strand, within Pf6N2E2_134at 182.582 kb on - strand, within Pf6N2E2_134at 182.582 kb on - strand, within Pf6N2E2_134at 183.027 kb on + strand, within Pf6N2E2_134at 183.071 kb on - strand, within Pf6N2E2_134at 183.192 kb on - strand, within Pf6N2E2_134at 183.283 kb on + strand, within Pf6N2E2_134at 183.283 kb on + strand, within Pf6N2E2_134at 183.283 kb on + strand, within Pf6N2E2_134at 183.284 kb on - strand, within Pf6N2E2_134at 183.284 kb on - strand, within Pf6N2E2_134at 183.284 kb on - strand, within Pf6N2E2_134at 183.487 kb on + strandat 183.487 kb on + strandat 183.508 kb on + strandat 183.508 kb on + strandat 183.508 kb on + strandat 183.509 kb on - strandat 183.509 kb on - strandat 183.509 kb on - strandat 183.528 kb on - strandat 183.839 kb on + strand, within Pf6N2E2_135at 183.839 kb on + strand, within Pf6N2E2_135at 183.839 kb on + strand, within Pf6N2E2_135at 183.839 kb on + strand, within Pf6N2E2_135at 183.840 kb on - strand, within Pf6N2E2_135at 184.106 kb on + strand, within Pf6N2E2_135at 184.239 kb on - strand, within Pf6N2E2_135

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Per-strain Table

Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M
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181,205 - +0.3
181,221 + -0.5
181,221 + +1.2
181,221 + -0.6
181,224 + -0.4
181,224 + -1.5
181,224 + +0.1
181,224 + +0.3
181,224 + -0.5
181,224 + +0.9
181,224 + +0.7
181,224 + +0.2
181,225 - +2.2
181,225 - +0.6
181,225 - -0.7
181,225 - +2.0
181,255 + -0.7
181,256 - +0.6
181,312 - +1.0
181,321 + +1.6
181,321 + -0.2
181,428 + Pf6N2E2_132 0.18 +1.2
181,428 + Pf6N2E2_132 0.18 +0.3
181,429 - Pf6N2E2_132 0.18 +0.5
181,429 - Pf6N2E2_132 0.18 +0.1
181,429 - Pf6N2E2_132 0.18 +0.5
181,519 + Pf6N2E2_132 0.50 +0.6
181,519 + Pf6N2E2_132 0.50 -0.3
181,564 + Pf6N2E2_132 0.66 +0.7
181,565 - Pf6N2E2_132 0.67 +0.2
181,565 - Pf6N2E2_132 0.67 -0.1
181,567 + Pf6N2E2_132 0.67 +0.6
181,567 + Pf6N2E2_132 0.67 -0.8
181,567 + Pf6N2E2_132 0.67 -0.7
181,568 - Pf6N2E2_132 0.68 +0.6
181,568 - Pf6N2E2_132 0.68 +1.3
181,568 - Pf6N2E2_132 0.68 +0.3
181,683 - +0.3
181,692 + +1.1
181,693 - +0.0
181,693 - -1.6
181,705 - +0.3
181,705 - -0.1
181,790 + Pf6N2E2_133 0.59 -1.1
181,811 + Pf6N2E2_133 0.73 +0.9
181,829 + Pf6N2E2_133 0.85 -0.6
181,851 + -0.7
181,851 + +0.7
181,851 + +0.3
181,851 + +0.7
181,852 - +0.2
181,852 - -0.3
181,852 - -0.4
181,852 - +0.6
181,852 - -0.8
181,879 + +0.7
181,879 + +0.1
181,879 + +0.0
181,880 - -0.9
182,188 + +0.4
182,320 + +1.2
182,320 + -0.7
182,392 + Pf6N2E2_134 0.14 +0.2
182,392 + Pf6N2E2_134 0.14 -0.3
182,393 - Pf6N2E2_134 0.15 +0.5
182,393 - Pf6N2E2_134 0.15 -1.8
182,416 + Pf6N2E2_134 0.16 -0.2
182,416 + Pf6N2E2_134 0.16 +0.1
182,417 - Pf6N2E2_134 0.16 -0.5
182,506 + Pf6N2E2_134 0.23 -0.2
182,506 + Pf6N2E2_134 0.23 -0.8
182,506 + Pf6N2E2_134 0.23 +0.2
182,506 + Pf6N2E2_134 0.23 +0.6
182,581 + Pf6N2E2_134 0.28 +0.0
182,582 - Pf6N2E2_134 0.28 -1.1
182,582 - Pf6N2E2_134 0.28 -2.6
183,027 + Pf6N2E2_134 0.61 -0.4
183,071 - Pf6N2E2_134 0.64 -0.7
183,192 - Pf6N2E2_134 0.73 -0.4
183,283 + Pf6N2E2_134 0.79 +0.6
183,283 + Pf6N2E2_134 0.79 -0.3
183,283 + Pf6N2E2_134 0.79 -0.1
183,284 - Pf6N2E2_134 0.79 +1.3
183,284 - Pf6N2E2_134 0.79 +0.1
183,284 - Pf6N2E2_134 0.79 +0.6
183,487 + -0.6
183,487 + +0.0
183,508 + +0.2
183,508 + -0.6
183,508 + +0.8
183,509 - -0.4
183,509 - -0.4
183,509 - +0.7
183,528 - +0.4
183,839 + Pf6N2E2_135 0.15 +0.8
183,839 + Pf6N2E2_135 0.15 -0.1
183,839 + Pf6N2E2_135 0.15 +0.6
183,839 + Pf6N2E2_135 0.15 -0.4
183,840 - Pf6N2E2_135 0.15 +0.7
184,106 + Pf6N2E2_135 0.37 +0.9
184,239 - Pf6N2E2_135 0.48 +1.1

Or see this region's nucleotide sequence