Experiment: Carbon source D-Glucose 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03135 and HSERO_RS03140 are separated by 48 nucleotides HSERO_RS03140 and HSERO_RS03145 are separated by 10 nucleotides HSERO_RS03145 and HSERO_RS03150 overlap by 4 nucleotides HSERO_RS03150 and HSERO_RS03155 overlap by 4 nucleotides
HSERO_RS03135: HSERO_RS03135 - diguanylate cyclase, at 686,209 to 687,471
_RS03135
HSERO_RS03140: HSERO_RS03140 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 687,520 to 688,005
_RS03140
HSERO_RS03145: HSERO_RS03145 - poly(A) polymerase, at 688,016 to 689,620
_RS03145
HSERO_RS03150: HSERO_RS03150 - phosphoserine phosphatase, at 689,617 to 690,300
_RS03150
HSERO_RS03155: HSERO_RS03155 - hypothetical protein, at 690,297 to 691,013
_RS03155
Position (kb)
688
689
690 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 687.077 kb on - strand, within HSERO_RS03135 at 687.106 kb on - strand, within HSERO_RS03135 at 687.131 kb on + strand, within HSERO_RS03135 at 687.182 kb on - strand, within HSERO_RS03135 at 687.346 kb on - strand at 687.396 kb on + strand at 687.477 kb on + strand at 687.487 kb on - strand at 688.021 kb on - strand at 688.145 kb on + strand at 688.493 kb on - strand, within HSERO_RS03145 at 688.493 kb on - strand, within HSERO_RS03145 at 688.498 kb on - strand, within HSERO_RS03145 at 688.576 kb on - strand, within HSERO_RS03145 at 688.674 kb on + strand, within HSERO_RS03145 at 688.674 kb on + strand, within HSERO_RS03145 at 688.682 kb on - strand, within HSERO_RS03145 at 688.682 kb on - strand, within HSERO_RS03145 at 688.682 kb on - strand, within HSERO_RS03145 at 688.682 kb on - strand, within HSERO_RS03145 at 688.682 kb on - strand, within HSERO_RS03145 at 688.878 kb on + strand, within HSERO_RS03145 at 688.984 kb on - strand, within HSERO_RS03145 at 689.256 kb on + strand, within HSERO_RS03145 at 689.298 kb on + strand at 689.349 kb on - strand, within HSERO_RS03145 at 689.444 kb on - strand, within HSERO_RS03145 at 689.499 kb on + strand at 690.049 kb on - strand, within HSERO_RS03150 at 690.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D-Glucose 20 mM remove 687,077 - HSERO_RS03135 0.69 +0.1 687,106 - HSERO_RS03135 0.71 -0.7 687,131 + HSERO_RS03135 0.73 +1.4 687,182 - HSERO_RS03135 0.77 -0.2 687,346 - +0.5 687,396 + +0.0 687,477 + -0.2 687,487 - +0.0 688,021 - -0.3 688,145 + +1.5 688,493 - HSERO_RS03145 0.30 +0.7 688,493 - HSERO_RS03145 0.30 +0.1 688,498 - HSERO_RS03145 0.30 +1.7 688,576 - HSERO_RS03145 0.35 +0.5 688,674 + HSERO_RS03145 0.41 -0.6 688,674 + HSERO_RS03145 0.41 +0.4 688,682 - HSERO_RS03145 0.41 -0.1 688,682 - HSERO_RS03145 0.41 +0.2 688,682 - HSERO_RS03145 0.41 +0.3 688,682 - HSERO_RS03145 0.41 +0.6 688,682 - HSERO_RS03145 0.41 +0.4 688,878 + HSERO_RS03145 0.54 +1.3 688,984 - HSERO_RS03145 0.60 +1.5 689,256 + HSERO_RS03145 0.77 +0.2 689,298 + +0.4 689,349 - HSERO_RS03145 0.83 +0.7 689,444 - HSERO_RS03145 0.89 +0.2 689,499 + +0.1 690,049 - HSERO_RS03150 0.63 -2.5 690,265 - -3.3
Or see this region's nucleotide sequence