Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_20065

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_20060 and AO356_20065 are separated by 65 nucleotidesAO356_20065 and AO356_20070 overlap by 4 nucleotidesAO356_20070 and AO356_20075 overlap by 1 nucleotides AO356_20060: AO356_20060 - competence protein ComEC, at 4,489,621 to 4,491,867 _20060 AO356_20065: AO356_20065 - biopolymer transporter ExbB, at 4,491,933 to 4,492,598 _20065 AO356_20070: AO356_20070 - biopolymer transporter ExbD, at 4,492,595 to 4,493,023 _20070 AO356_20075: AO356_20075 - tetraacyldisaccharide 4'-kinase, at 4,493,023 to 4,494,033 _20075 Position (kb) 4491 4492 4493Strain fitness (log2 ratio) -1 0 1at 4491.166 kb on + strand, within AO356_20060at 4491.166 kb on + strand, within AO356_20060at 4491.215 kb on + strand, within AO356_20060at 4491.216 kb on - strand, within AO356_20060at 4491.460 kb on - strand, within AO356_20060at 4491.742 kb on + strandat 4491.743 kb on - strandat 4491.743 kb on - strandat 4491.743 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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4,491,166 + AO356_20060 0.69 -1.4
4,491,166 + AO356_20060 0.69 -0.7
4,491,215 + AO356_20060 0.71 +0.3
4,491,216 - AO356_20060 0.71 +0.2
4,491,460 - AO356_20060 0.82 +0.5
4,491,742 + -0.1
4,491,743 - -0.5
4,491,743 - -1.2
4,491,743 - +0.9

Or see this region's nucleotide sequence