Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36975 and RR42_RS36980 are separated by 22 nucleotides RR42_RS36980 and RR42_RS36985 are separated by 106 nucleotides RR42_RS36985 and RR42_RS36990 are separated by 245 nucleotides RR42_RS36990 and RR42_RS36995 are separated by 38 nucleotides
RR42_RS36975: RR42_RS36975 - polar amino acid ABC transporter permease, at 3,751,759 to 3,752,394
_RS36975
RR42_RS36980: RR42_RS36980 - amino acid ABC transporter permease, at 3,752,417 to 3,753,091
_RS36980
RR42_RS36985: RR42_RS36985 - amino acid ABC transporter substrate-binding protein, at 3,753,198 to 3,754,010
_RS36985
RR42_RS36990: RR42_RS36990 - hemolysin III, at 3,754,256 to 3,754,873
_RS36990
RR42_RS36995: RR42_RS36995 - hypothetical protein, at 3,754,912 to 3,755,346
_RS36995
Position (kb)
3753
3754
3755 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 3752.499 kb on + strand, within RR42_RS36980 at 3752.704 kb on + strand, within RR42_RS36980 at 3752.880 kb on + strand, within RR42_RS36980 at 3752.881 kb on - strand, within RR42_RS36980 at 3752.881 kb on - strand, within RR42_RS36980 at 3752.928 kb on + strand, within RR42_RS36980 at 3752.928 kb on + strand, within RR42_RS36980 at 3752.929 kb on - strand, within RR42_RS36980 at 3753.167 kb on + strand at 3753.183 kb on - strand at 3753.271 kb on + strand at 3753.271 kb on + strand at 3753.271 kb on + strand at 3753.272 kb on - strand at 3753.370 kb on + strand, within RR42_RS36985 at 3753.370 kb on + strand, within RR42_RS36985 at 3753.371 kb on - strand, within RR42_RS36985 at 3753.371 kb on - strand, within RR42_RS36985 at 3753.371 kb on - strand, within RR42_RS36985 at 3753.371 kb on - strand, within RR42_RS36985 at 3753.518 kb on - strand, within RR42_RS36985 at 3753.665 kb on - strand, within RR42_RS36985 at 3753.854 kb on + strand, within RR42_RS36985 at 3753.855 kb on - strand, within RR42_RS36985 at 3754.022 kb on + strand at 3754.023 kb on - strand at 3754.108 kb on + strand at 3754.108 kb on + strand at 3754.108 kb on + strand at 3754.182 kb on + strand at 3754.183 kb on - strand at 3754.190 kb on + strand at 3754.239 kb on - strand at 3754.259 kb on + strand at 3754.260 kb on - strand at 3754.260 kb on - strand at 3754.284 kb on - strand at 3754.284 kb on - strand at 3754.333 kb on + strand, within RR42_RS36990 at 3754.334 kb on - strand, within RR42_RS36990 at 3754.353 kb on - strand, within RR42_RS36990 at 3754.413 kb on - strand, within RR42_RS36990 at 3754.478 kb on + strand, within RR42_RS36990 at 3754.490 kb on + strand, within RR42_RS36990 at 3754.490 kb on + strand, within RR42_RS36990 at 3754.490 kb on + strand, within RR42_RS36990 at 3754.491 kb on - strand, within RR42_RS36990 at 3754.511 kb on + strand, within RR42_RS36990 at 3754.511 kb on + strand, within RR42_RS36990 at 3754.511 kb on + strand, within RR42_RS36990 at 3754.512 kb on - strand, within RR42_RS36990 at 3754.512 kb on - strand, within RR42_RS36990 at 3754.514 kb on - strand, within RR42_RS36990 at 3754.514 kb on - strand, within RR42_RS36990 at 3754.649 kb on + strand, within RR42_RS36990 at 3754.649 kb on + strand, within RR42_RS36990 at 3754.649 kb on + strand, within RR42_RS36990 at 3754.649 kb on + strand, within RR42_RS36990 at 3754.649 kb on + strand, within RR42_RS36990 at 3754.650 kb on - strand, within RR42_RS36990 at 3754.650 kb on - strand, within RR42_RS36990 at 3754.650 kb on - strand, within RR42_RS36990 at 3754.650 kb on - strand, within RR42_RS36990 at 3754.650 kb on - strand, within RR42_RS36990 at 3754.721 kb on + strand, within RR42_RS36990 at 3754.722 kb on - strand, within RR42_RS36990 at 3754.748 kb on + strand, within RR42_RS36990 at 3754.749 kb on - strand, within RR42_RS36990 at 3754.751 kb on - strand, within RR42_RS36990 at 3754.751 kb on - strand, within RR42_RS36990 at 3754.863 kb on - strand at 3754.882 kb on + strand at 3754.882 kb on + strand at 3754.882 kb on + strand at 3754.883 kb on - strand at 3754.923 kb on - strand at 3754.979 kb on + strand, within RR42_RS36995
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 3,752,499 + RR42_RS36980 0.12 +1.2 3,752,704 + RR42_RS36980 0.43 +0.5 3,752,880 + RR42_RS36980 0.69 +0.7 3,752,881 - RR42_RS36980 0.69 +0.8 3,752,881 - RR42_RS36980 0.69 -2.3 3,752,928 + RR42_RS36980 0.76 +1.4 3,752,928 + RR42_RS36980 0.76 +0.1 3,752,929 - RR42_RS36980 0.76 +0.1 3,753,167 + +1.8 3,753,183 - +1.9 3,753,271 + +1.9 3,753,271 + +0.7 3,753,271 + +1.8 3,753,272 - -0.9 3,753,370 + RR42_RS36985 0.21 +1.2 3,753,370 + RR42_RS36985 0.21 +1.6 3,753,371 - RR42_RS36985 0.21 +2.1 3,753,371 - RR42_RS36985 0.21 +0.4 3,753,371 - RR42_RS36985 0.21 +0.8 3,753,371 - RR42_RS36985 0.21 +1.9 3,753,518 - RR42_RS36985 0.39 +0.9 3,753,665 - RR42_RS36985 0.57 +1.3 3,753,854 + RR42_RS36985 0.81 +2.2 3,753,855 - RR42_RS36985 0.81 +1.2 3,754,022 + +2.5 3,754,023 - -1.7 3,754,108 + +2.8 3,754,108 + +1.1 3,754,108 + +2.4 3,754,182 + +4.4 3,754,183 - -3.5 3,754,190 + +2.1 3,754,239 - -3.3 3,754,259 + -5.0 3,754,260 - -2.4 3,754,260 - -3.8 3,754,284 - -3.6 3,754,284 - -2.8 3,754,333 + RR42_RS36990 0.12 -3.0 3,754,334 - RR42_RS36990 0.13 -5.5 3,754,353 - RR42_RS36990 0.16 -3.0 3,754,413 - RR42_RS36990 0.25 -3.6 3,754,478 + RR42_RS36990 0.36 -1.9 3,754,490 + RR42_RS36990 0.38 -2.8 3,754,490 + RR42_RS36990 0.38 -3.8 3,754,490 + RR42_RS36990 0.38 -4.4 3,754,491 - RR42_RS36990 0.38 -5.4 3,754,511 + RR42_RS36990 0.41 -3.3 3,754,511 + RR42_RS36990 0.41 -4.2 3,754,511 + RR42_RS36990 0.41 -4.2 3,754,512 - RR42_RS36990 0.41 -3.6 3,754,512 - RR42_RS36990 0.41 -4.8 3,754,514 - RR42_RS36990 0.42 -2.4 3,754,514 - RR42_RS36990 0.42 -1.2 3,754,649 + RR42_RS36990 0.64 -2.6 3,754,649 + RR42_RS36990 0.64 -3.4 3,754,649 + RR42_RS36990 0.64 -5.8 3,754,649 + RR42_RS36990 0.64 -2.9 3,754,649 + RR42_RS36990 0.64 -3.3 3,754,650 - RR42_RS36990 0.64 -4.5 3,754,650 - RR42_RS36990 0.64 -2.6 3,754,650 - RR42_RS36990 0.64 -0.5 3,754,650 - RR42_RS36990 0.64 -4.2 3,754,650 - RR42_RS36990 0.64 -4.5 3,754,721 + RR42_RS36990 0.75 -3.1 3,754,722 - RR42_RS36990 0.75 -4.1 3,754,748 + RR42_RS36990 0.80 -2.6 3,754,749 - RR42_RS36990 0.80 -4.2 3,754,751 - RR42_RS36990 0.80 -3.5 3,754,751 - RR42_RS36990 0.80 -1.5 3,754,863 - -3.2 3,754,882 + +0.5 3,754,882 + +2.3 3,754,882 + +1.1 3,754,883 - +0.6 3,754,923 - -0.7 3,754,979 + RR42_RS36995 0.15 -0.8
Or see this region's nucleotide sequence