Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35720 and RR42_RS35725 are separated by 7 nucleotides RR42_RS35725 and RR42_RS35730 are separated by 222 nucleotides RR42_RS35730 and RR42_RS35735 are separated by 165 nucleotides RR42_RS35735 and RR42_RS35740 are separated by 54 nucleotides
RR42_RS35720: RR42_RS35720 - flagellar basal body rod protein FlgC, at 3,464,228 to 3,464,635
_RS35720
RR42_RS35725: RR42_RS35725 - flagellar biosynthesis protein FlgB, at 3,464,643 to 3,465,053
_RS35725
RR42_RS35730: RR42_RS35730 - flagellar basal body P-ring biosynthesis protein FlgA, at 3,465,276 to 3,465,995
_RS35730
RR42_RS35735: RR42_RS35735 - flagellar biosynthesis protein FlgM, at 3,466,161 to 3,466,466
_RS35735
RR42_RS35740: RR42_RS35740 - flagellar biosynthesis protein FlgN, at 3,466,521 to 3,466,979
_RS35740
Position (kb)
3465
3466 Strain fitness (log2 ratio)
-2
-1
0
1 at 3464.298 kb on + strand, within RR42_RS35720 at 3464.298 kb on + strand, within RR42_RS35720 at 3464.679 kb on - strand at 3464.942 kb on - strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3465.197 kb on + strand at 3465.197 kb on + strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.271 kb on + strand at 3465.271 kb on + strand at 3465.271 kb on + strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.272 kb on - strand at 3465.464 kb on - strand, within RR42_RS35730 at 3465.591 kb on + strand, within RR42_RS35730 at 3465.592 kb on - strand, within RR42_RS35730 at 3465.624 kb on + strand, within RR42_RS35730 at 3465.625 kb on - strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.627 kb on + strand, within RR42_RS35730 at 3465.628 kb on - strand, within RR42_RS35730 at 3465.628 kb on - strand, within RR42_RS35730 at 3465.628 kb on - strand, within RR42_RS35730 at 3466.035 kb on + strand at 3466.036 kb on - strand at 3466.055 kb on + strand at 3466.055 kb on + strand at 3466.055 kb on + strand at 3466.055 kb on + strand at 3466.108 kb on - strand at 3466.108 kb on - strand at 3466.132 kb on - strand at 3466.480 kb on + strand at 3466.507 kb on - strand at 3466.511 kb on + strand at 3466.512 kb on - strand at 3466.586 kb on - strand, within RR42_RS35740 at 3466.611 kb on - strand, within RR42_RS35740 at 3466.611 kb on - strand, within RR42_RS35740 at 3466.921 kb on - strand, within RR42_RS35740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 3,464,298 + RR42_RS35720 0.17 -1.5 3,464,298 + RR42_RS35720 0.17 +0.5 3,464,679 - -1.1 3,464,942 - RR42_RS35725 0.73 -0.8 3,464,951 + RR42_RS35725 0.75 -1.2 3,464,951 + RR42_RS35725 0.75 -2.6 3,464,951 + RR42_RS35725 0.75 +1.1 3,465,197 + +1.6 3,465,197 + -1.2 3,465,203 - +0.8 3,465,203 - -0.3 3,465,203 - +0.8 3,465,203 - +0.6 3,465,271 + +0.4 3,465,271 + +0.3 3,465,271 + -0.7 3,465,272 - -0.2 3,465,272 - +0.4 3,465,272 - -0.5 3,465,272 - +0.4 3,465,272 - -1.1 3,465,272 - -2.0 3,465,464 - RR42_RS35730 0.26 +0.0 3,465,591 + RR42_RS35730 0.44 +0.2 3,465,592 - RR42_RS35730 0.44 +0.9 3,465,624 + RR42_RS35730 0.48 +0.6 3,465,625 - RR42_RS35730 0.48 +0.9 3,465,627 + RR42_RS35730 0.49 +0.4 3,465,627 + RR42_RS35730 0.49 -0.0 3,465,627 + RR42_RS35730 0.49 +1.0 3,465,627 + RR42_RS35730 0.49 +1.4 3,465,628 - RR42_RS35730 0.49 -1.0 3,465,628 - RR42_RS35730 0.49 -0.1 3,465,628 - RR42_RS35730 0.49 +0.5 3,466,035 + -1.2 3,466,036 - -1.5 3,466,055 + -0.8 3,466,055 + -0.3 3,466,055 + -0.1 3,466,055 + +0.3 3,466,108 - -2.0 3,466,108 - -1.6 3,466,132 - +0.2 3,466,480 + -1.0 3,466,507 - +1.0 3,466,511 + -0.0 3,466,512 - +0.4 3,466,586 - RR42_RS35740 0.14 -0.2 3,466,611 - RR42_RS35740 0.20 -0.0 3,466,611 - RR42_RS35740 0.20 -0.4 3,466,921 - RR42_RS35740 0.87 -0.6
Or see this region's nucleotide sequence