Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33325 and RR42_RS33330 are separated by 61 nucleotides RR42_RS33330 and RR42_RS33335 are separated by 191 nucleotides
RR42_RS33325: RR42_RS33325 - LacI family transcriptional regulator, at 2,891,888 to 2,892,892
_RS33325
RR42_RS33330: RR42_RS33330 - hydroxyacid dehydrogenase, at 2,892,954 to 2,893,892
_RS33330
RR42_RS33335: RR42_RS33335 - hypothetical protein, at 2,894,084 to 2,894,464
_RS33335
Position (kb)
2892
2893
2894 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2892.182 kb on + strand, within RR42_RS33325 at 2892.233 kb on + strand, within RR42_RS33325 at 2892.234 kb on - strand, within RR42_RS33325 at 2892.234 kb on - strand, within RR42_RS33325 at 2892.248 kb on + strand, within RR42_RS33325 at 2892.248 kb on + strand, within RR42_RS33325 at 2892.248 kb on + strand, within RR42_RS33325 at 2892.248 kb on + strand, within RR42_RS33325 at 2892.249 kb on - strand, within RR42_RS33325 at 2892.249 kb on - strand, within RR42_RS33325 at 2892.249 kb on - strand, within RR42_RS33325 at 2892.249 kb on - strand, within RR42_RS33325 at 2892.310 kb on - strand, within RR42_RS33325 at 2892.383 kb on + strand, within RR42_RS33325 at 2892.384 kb on - strand, within RR42_RS33325 at 2892.384 kb on - strand, within RR42_RS33325 at 2892.439 kb on - strand, within RR42_RS33325 at 2892.439 kb on - strand, within RR42_RS33325 at 2892.470 kb on + strand, within RR42_RS33325 at 2892.471 kb on - strand, within RR42_RS33325 at 2892.851 kb on + strand at 2893.020 kb on + strand at 2893.020 kb on + strand at 2893.234 kb on - strand, within RR42_RS33330 at 2893.235 kb on + strand, within RR42_RS33330 at 2893.235 kb on + strand, within RR42_RS33330 at 2893.235 kb on + strand, within RR42_RS33330 at 2893.236 kb on - strand, within RR42_RS33330 at 2893.236 kb on - strand, within RR42_RS33330 at 2893.465 kb on - strand, within RR42_RS33330 at 2893.637 kb on + strand, within RR42_RS33330 at 2893.787 kb on + strand, within RR42_RS33330 at 2893.788 kb on - strand, within RR42_RS33330 at 2893.890 kb on + strand at 2893.891 kb on - strand at 2893.891 kb on - strand at 2893.987 kb on + strand at 2893.988 kb on - strand at 2893.988 kb on - strand at 2893.988 kb on - strand at 2894.004 kb on - strand at 2894.004 kb on - strand at 2894.010 kb on + strand at 2894.011 kb on - strand at 2894.243 kb on + strand, within RR42_RS33335 at 2894.243 kb on + strand, within RR42_RS33335 at 2894.243 kb on + strand, within RR42_RS33335 at 2894.243 kb on + strand, within RR42_RS33335 at 2894.243 kb on + strand, within RR42_RS33335 at 2894.243 kb on + strand, within RR42_RS33335 at 2894.243 kb on + strand, within RR42_RS33335 at 2894.244 kb on - strand, within RR42_RS33335 at 2894.244 kb on - strand, within RR42_RS33335 at 2894.246 kb on + strand, within RR42_RS33335 at 2894.246 kb on + strand, within RR42_RS33335 at 2894.247 kb on - strand, within RR42_RS33335 at 2894.247 kb on - strand, within RR42_RS33335 at 2894.247 kb on - strand, within RR42_RS33335 at 2894.296 kb on + strand, within RR42_RS33335 at 2894.297 kb on - strand, within RR42_RS33335 at 2894.378 kb on - strand, within RR42_RS33335 at 2894.432 kb on + strand at 2894.432 kb on + strand at 2894.432 kb on + strand at 2894.433 kb on - strand at 2894.600 kb on + strand at 2894.609 kb on + strand at 2894.610 kb on - strand at 2894.660 kb on + strand at 2894.660 kb on + strand at 2894.744 kb on + strand at 2894.744 kb on + strand at 2894.744 kb on + strand at 2894.745 kb on - strand at 2894.745 kb on - strand at 2894.750 kb on + strand at 2894.788 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,892,182 + RR42_RS33325 0.29 -0.0 2,892,233 + RR42_RS33325 0.34 -1.2 2,892,234 - RR42_RS33325 0.34 -1.1 2,892,234 - RR42_RS33325 0.34 -1.7 2,892,248 + RR42_RS33325 0.36 -0.7 2,892,248 + RR42_RS33325 0.36 +0.1 2,892,248 + RR42_RS33325 0.36 -0.2 2,892,248 + RR42_RS33325 0.36 -0.9 2,892,249 - RR42_RS33325 0.36 -0.8 2,892,249 - RR42_RS33325 0.36 +0.4 2,892,249 - RR42_RS33325 0.36 -2.1 2,892,249 - RR42_RS33325 0.36 +0.0 2,892,310 - RR42_RS33325 0.42 +0.4 2,892,383 + RR42_RS33325 0.49 +0.2 2,892,384 - RR42_RS33325 0.49 +0.3 2,892,384 - RR42_RS33325 0.49 +0.1 2,892,439 - RR42_RS33325 0.55 +1.1 2,892,439 - RR42_RS33325 0.55 +0.0 2,892,470 + RR42_RS33325 0.58 -0.5 2,892,471 - RR42_RS33325 0.58 +0.4 2,892,851 + +0.1 2,893,020 + +0.4 2,893,020 + +1.1 2,893,234 - RR42_RS33330 0.30 -0.8 2,893,235 + RR42_RS33330 0.30 -3.0 2,893,235 + RR42_RS33330 0.30 +0.2 2,893,235 + RR42_RS33330 0.30 +0.7 2,893,236 - RR42_RS33330 0.30 +0.0 2,893,236 - RR42_RS33330 0.30 -0.3 2,893,465 - RR42_RS33330 0.54 -2.5 2,893,637 + RR42_RS33330 0.73 +0.0 2,893,787 + RR42_RS33330 0.89 -2.9 2,893,788 - RR42_RS33330 0.89 +0.5 2,893,890 + +2.0 2,893,891 - -0.1 2,893,891 - -0.1 2,893,987 + -0.1 2,893,988 - +0.5 2,893,988 - +0.3 2,893,988 - +1.0 2,894,004 - -0.0 2,894,004 - +0.9 2,894,010 + -1.2 2,894,011 - -1.5 2,894,243 + RR42_RS33335 0.42 +1.0 2,894,243 + RR42_RS33335 0.42 +0.4 2,894,243 + RR42_RS33335 0.42 -0.2 2,894,243 + RR42_RS33335 0.42 -0.5 2,894,243 + RR42_RS33335 0.42 +0.4 2,894,243 + RR42_RS33335 0.42 -2.2 2,894,243 + RR42_RS33335 0.42 -0.0 2,894,244 - RR42_RS33335 0.42 +0.3 2,894,244 - RR42_RS33335 0.42 +0.3 2,894,246 + RR42_RS33335 0.43 +0.5 2,894,246 + RR42_RS33335 0.43 -0.5 2,894,247 - RR42_RS33335 0.43 -0.3 2,894,247 - RR42_RS33335 0.43 +0.6 2,894,247 - RR42_RS33335 0.43 +1.0 2,894,296 + RR42_RS33335 0.56 -1.5 2,894,297 - RR42_RS33335 0.56 +1.4 2,894,378 - RR42_RS33335 0.77 +0.2 2,894,432 + +0.2 2,894,432 + -0.2 2,894,432 + -0.2 2,894,433 - +0.5 2,894,600 + -2.1 2,894,609 + +0.7 2,894,610 - +0.4 2,894,660 + -0.2 2,894,660 + -0.1 2,894,744 + +0.3 2,894,744 + +1.1 2,894,744 + -1.0 2,894,745 - -0.4 2,894,745 - +0.2 2,894,750 + +1.1 2,894,788 + +1.2
Or see this region's nucleotide sequence