Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33075 and RR42_RS33080 are separated by 24 nucleotides RR42_RS33080 and RR42_RS33085 are separated by 588 nucleotides
RR42_RS33075: RR42_RS33075 - dihydrodipicolinate synthetase, at 2,826,307 to 2,827,248
_RS33075
RR42_RS33080: RR42_RS33080 - IclR family transcriptional regulator, at 2,827,273 to 2,828,109
_RS33080
RR42_RS33085: RR42_RS33085 - ligand-gated channel protein, at 2,828,698 to 2,830,917
_RS33085
Position (kb)
2827
2828
2829 Strain fitness (log2 ratio)
-2
-1
0
1 at 2826.486 kb on - strand, within RR42_RS33075 at 2826.647 kb on + strand, within RR42_RS33075 at 2826.648 kb on - strand, within RR42_RS33075 at 2826.717 kb on - strand, within RR42_RS33075 at 2826.717 kb on - strand, within RR42_RS33075 at 2826.758 kb on + strand, within RR42_RS33075 at 2826.758 kb on + strand, within RR42_RS33075 at 2826.759 kb on - strand, within RR42_RS33075 at 2826.797 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.822 kb on - strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.872 kb on + strand, within RR42_RS33075 at 2826.872 kb on + strand, within RR42_RS33075 at 2826.872 kb on + strand, within RR42_RS33075 at 2827.101 kb on + strand, within RR42_RS33075 at 2827.187 kb on + strand at 2827.187 kb on + strand at 2827.188 kb on - strand at 2827.188 kb on - strand at 2827.188 kb on - strand at 2827.239 kb on + strand at 2827.240 kb on - strand at 2827.404 kb on + strand, within RR42_RS33080 at 2827.404 kb on + strand, within RR42_RS33080 at 2827.405 kb on - strand, within RR42_RS33080 at 2827.405 kb on - strand, within RR42_RS33080 at 2827.481 kb on + strand, within RR42_RS33080 at 2827.481 kb on + strand, within RR42_RS33080 at 2827.481 kb on + strand, within RR42_RS33080 at 2827.481 kb on + strand, within RR42_RS33080 at 2827.481 kb on + strand, within RR42_RS33080 at 2827.482 kb on - strand, within RR42_RS33080 at 2827.517 kb on + strand, within RR42_RS33080 at 2827.517 kb on + strand, within RR42_RS33080 at 2827.518 kb on - strand, within RR42_RS33080 at 2827.518 kb on - strand, within RR42_RS33080 at 2827.518 kb on - strand, within RR42_RS33080 at 2827.656 kb on - strand, within RR42_RS33080 at 2827.926 kb on - strand, within RR42_RS33080 at 2828.134 kb on - strand at 2828.321 kb on - strand at 2828.461 kb on + strand at 2828.462 kb on - strand at 2828.462 kb on - strand at 2828.519 kb on - strand at 2828.519 kb on - strand at 2828.524 kb on + strand at 2828.524 kb on + strand at 2828.524 kb on + strand at 2828.525 kb on - strand at 2828.530 kb on + strand at 2828.530 kb on + strand at 2828.530 kb on + strand at 2828.531 kb on - strand at 2828.609 kb on + strand at 2828.610 kb on - strand at 2828.610 kb on - strand at 2828.610 kb on - strand at 2828.610 kb on - strand at 2828.611 kb on + strand at 2828.753 kb on - strand at 2828.887 kb on + strand at 2828.888 kb on - strand at 2828.888 kb on - strand at 2828.913 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,826,486 - RR42_RS33075 0.19 -0.5 2,826,647 + RR42_RS33075 0.36 +0.6 2,826,648 - RR42_RS33075 0.36 +0.3 2,826,717 - RR42_RS33075 0.44 -0.3 2,826,717 - RR42_RS33075 0.44 -0.7 2,826,758 + RR42_RS33075 0.48 +0.0 2,826,758 + RR42_RS33075 0.48 -2.4 2,826,759 - RR42_RS33075 0.48 +1.6 2,826,797 + RR42_RS33075 0.52 +0.5 2,826,821 + RR42_RS33075 0.55 -0.8 2,826,821 + RR42_RS33075 0.55 -1.0 2,826,821 + RR42_RS33075 0.55 -1.2 2,826,821 + RR42_RS33075 0.55 -1.4 2,826,821 + RR42_RS33075 0.55 -0.7 2,826,822 - RR42_RS33075 0.55 +0.9 2,826,825 + RR42_RS33075 0.55 +1.2 2,826,825 + RR42_RS33075 0.55 -2.0 2,826,825 + RR42_RS33075 0.55 +0.8 2,826,825 + RR42_RS33075 0.55 -0.0 2,826,825 + RR42_RS33075 0.55 -0.1 2,826,825 + RR42_RS33075 0.55 -1.1 2,826,825 + RR42_RS33075 0.55 -0.3 2,826,825 + RR42_RS33075 0.55 -1.0 2,826,825 + RR42_RS33075 0.55 +1.4 2,826,826 - RR42_RS33075 0.55 -0.8 2,826,826 - RR42_RS33075 0.55 -2.1 2,826,826 - RR42_RS33075 0.55 +1.0 2,826,826 - RR42_RS33075 0.55 -0.2 2,826,826 - RR42_RS33075 0.55 +0.5 2,826,872 + RR42_RS33075 0.60 -0.3 2,826,872 + RR42_RS33075 0.60 +0.8 2,826,872 + RR42_RS33075 0.60 +0.6 2,827,101 + RR42_RS33075 0.84 -0.9 2,827,187 + -1.3 2,827,187 + +1.4 2,827,188 - -1.0 2,827,188 - -1.6 2,827,188 - +0.0 2,827,239 + -0.1 2,827,240 - -1.1 2,827,404 + RR42_RS33080 0.16 -0.4 2,827,404 + RR42_RS33080 0.16 +0.1 2,827,405 - RR42_RS33080 0.16 -2.3 2,827,405 - RR42_RS33080 0.16 -0.2 2,827,481 + RR42_RS33080 0.25 -1.4 2,827,481 + RR42_RS33080 0.25 -2.0 2,827,481 + RR42_RS33080 0.25 -0.3 2,827,481 + RR42_RS33080 0.25 +0.3 2,827,481 + RR42_RS33080 0.25 -2.3 2,827,482 - RR42_RS33080 0.25 -2.1 2,827,517 + RR42_RS33080 0.29 -0.6 2,827,517 + RR42_RS33080 0.29 -1.0 2,827,518 - RR42_RS33080 0.29 -0.4 2,827,518 - RR42_RS33080 0.29 -1.0 2,827,518 - RR42_RS33080 0.29 -1.5 2,827,656 - RR42_RS33080 0.46 -2.3 2,827,926 - RR42_RS33080 0.78 -1.8 2,828,134 - -0.4 2,828,321 - -2.3 2,828,461 + +1.3 2,828,462 - -0.6 2,828,462 - -2.6 2,828,519 - -2.2 2,828,519 - -0.5 2,828,524 + -1.5 2,828,524 + +0.1 2,828,524 + +0.5 2,828,525 - -0.2 2,828,530 + -1.9 2,828,530 + -0.7 2,828,530 + -1.4 2,828,531 - +0.1 2,828,609 + -1.5 2,828,610 - -0.5 2,828,610 - -2.0 2,828,610 - -2.2 2,828,610 - +0.5 2,828,611 + -2.2 2,828,753 - -0.2 2,828,887 + +1.2 2,828,888 - -0.8 2,828,888 - +0.0 2,828,913 + -0.4
Or see this region's nucleotide sequence