Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31695

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31685 and RR42_RS31690 are separated by 226 nucleotidesRR42_RS31690 and RR42_RS31695 are separated by 63 nucleotidesRR42_RS31695 and RR42_RS31700 are separated by 46 nucleotidesRR42_RS31700 and RR42_RS31705 are separated by 8 nucleotides RR42_RS31685: RR42_RS31685 - Asp/Glu/hydantoin racemase, at 2,488,467 to 2,489,087 _RS31685 RR42_RS31690: RR42_RS31690 - 4-oxalocrotonate tautomerase, at 2,489,314 to 2,489,727 _RS31690 RR42_RS31695: RR42_RS31695 - hypothetical protein, at 2,489,791 to 2,490,201 _RS31695 RR42_RS31700: RR42_RS31700 - esterase, at 2,490,248 to 2,491,141 _RS31700 RR42_RS31705: RR42_RS31705 - MBL fold metallo-hydrolase, at 2,491,150 to 2,492,133 _RS31705 Position (kb) 2489 2490 2491Strain fitness (log2 ratio) -2 -1 0 1 2at 2488.839 kb on + strand, within RR42_RS31685at 2488.839 kb on + strand, within RR42_RS31685at 2488.839 kb on + strand, within RR42_RS31685at 2488.951 kb on + strand, within RR42_RS31685at 2488.952 kb on - strand, within RR42_RS31685at 2488.979 kb on + strand, within RR42_RS31685at 2489.069 kb on - strandat 2489.069 kb on - strandat 2489.069 kb on - strandat 2489.087 kb on + strandat 2489.088 kb on - strandat 2489.094 kb on - strandat 2489.283 kb on + strandat 2489.315 kb on + strandat 2489.315 kb on + strandat 2489.316 kb on - strandat 2489.399 kb on + strand, within RR42_RS31690at 2489.400 kb on - strand, within RR42_RS31690at 2489.400 kb on - strand, within RR42_RS31690at 2489.415 kb on + strand, within RR42_RS31690at 2489.518 kb on + strand, within RR42_RS31690at 2489.519 kb on - strand, within RR42_RS31690at 2489.697 kb on + strandat 2489.797 kb on - strandat 2489.797 kb on - strandat 2489.976 kb on - strand, within RR42_RS31695at 2490.024 kb on - strand, within RR42_RS31695at 2490.024 kb on - strand, within RR42_RS31695at 2490.196 kb on - strandat 2490.223 kb on + strandat 2490.224 kb on - strandat 2490.426 kb on - strand, within RR42_RS31700at 2490.525 kb on + strand, within RR42_RS31700at 2490.525 kb on + strand, within RR42_RS31700at 2490.612 kb on - strand, within RR42_RS31700at 2490.635 kb on + strand, within RR42_RS31700at 2490.636 kb on - strand, within RR42_RS31700at 2490.780 kb on + strand, within RR42_RS31700at 2490.841 kb on + strand, within RR42_RS31700at 2490.841 kb on + strand, within RR42_RS31700at 2490.972 kb on - strand, within RR42_RS31700at 2490.972 kb on - strand, within RR42_RS31700at 2490.972 kb on - strand, within RR42_RS31700at 2490.972 kb on - strand, within RR42_RS31700at 2491.001 kb on + strand, within RR42_RS31700at 2491.134 kb on + strandat 2491.135 kb on - strandat 2491.156 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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2,488,839 + RR42_RS31685 0.60 -0.4
2,488,839 + RR42_RS31685 0.60 -1.0
2,488,839 + RR42_RS31685 0.60 +0.0
2,488,951 + RR42_RS31685 0.78 -1.0
2,488,952 - RR42_RS31685 0.78 -1.2
2,488,979 + RR42_RS31685 0.82 +1.4
2,489,069 - -0.6
2,489,069 - +0.3
2,489,069 - -1.5
2,489,087 + -2.0
2,489,088 - +0.3
2,489,094 - -0.2
2,489,283 + +0.0
2,489,315 + -0.2
2,489,315 + -0.1
2,489,316 - +0.4
2,489,399 + RR42_RS31690 0.21 +0.5
2,489,400 - RR42_RS31690 0.21 +1.5
2,489,400 - RR42_RS31690 0.21 +0.5
2,489,415 + RR42_RS31690 0.24 -0.1
2,489,518 + RR42_RS31690 0.49 -0.1
2,489,519 - RR42_RS31690 0.50 +0.4
2,489,697 + +1.0
2,489,797 - +0.3
2,489,797 - +2.1
2,489,976 - RR42_RS31695 0.45 +0.8
2,490,024 - RR42_RS31695 0.57 +1.5
2,490,024 - RR42_RS31695 0.57 +0.7
2,490,196 - -0.2
2,490,223 + -0.1
2,490,224 - -0.0
2,490,426 - RR42_RS31700 0.20 -0.8
2,490,525 + RR42_RS31700 0.31 -0.2
2,490,525 + RR42_RS31700 0.31 +0.6
2,490,612 - RR42_RS31700 0.41 -0.4
2,490,635 + RR42_RS31700 0.43 +0.4
2,490,636 - RR42_RS31700 0.43 +0.6
2,490,780 + RR42_RS31700 0.60 +0.1
2,490,841 + RR42_RS31700 0.66 +0.1
2,490,841 + RR42_RS31700 0.66 +0.2
2,490,972 - RR42_RS31700 0.81 +1.1
2,490,972 - RR42_RS31700 0.81 -1.8
2,490,972 - RR42_RS31700 0.81 -2.6
2,490,972 - RR42_RS31700 0.81 -1.3
2,491,001 + RR42_RS31700 0.84 +0.7
2,491,134 + +0.2
2,491,135 - -0.7
2,491,156 + -0.4

Or see this region's nucleotide sequence