Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31385

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31375 and RR42_RS31380 are separated by 1 nucleotidesRR42_RS31380 and RR42_RS31385 are separated by 80 nucleotidesRR42_RS31385 and RR42_RS31390 are separated by 228 nucleotides RR42_RS31375: RR42_RS31375 - succinyl-CoA synthetase subunit beta, at 2,415,790 to 2,417,022 _RS31375 RR42_RS31380: RR42_RS31380 - succinate--CoA ligase, at 2,417,024 to 2,417,908 _RS31380 RR42_RS31385: RR42_RS31385 - GntR family transcriptional regulator, at 2,417,989 to 2,418,708 _RS31385 RR42_RS31390: RR42_RS31390 - 2-dehydropantoate 2-reductase, at 2,418,937 to 2,419,911 _RS31390 Position (kb) 2417 2418 2419Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2417.006 kb on + strandat 2417.006 kb on + strandat 2417.006 kb on + strandat 2417.006 kb on + strandat 2417.007 kb on - strandat 2417.115 kb on - strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.237 kb on + strand, within RR42_RS31380at 2417.238 kb on - strand, within RR42_RS31380at 2417.238 kb on - strand, within RR42_RS31380at 2417.238 kb on - strand, within RR42_RS31380at 2417.361 kb on - strand, within RR42_RS31380at 2417.361 kb on - strand, within RR42_RS31380at 2417.446 kb on + strand, within RR42_RS31380at 2417.446 kb on + strand, within RR42_RS31380at 2417.498 kb on + strand, within RR42_RS31380at 2417.735 kb on - strand, within RR42_RS31380at 2417.806 kb on + strand, within RR42_RS31380at 2417.907 kb on - strandat 2417.939 kb on + strandat 2417.940 kb on - strandat 2417.940 kb on - strandat 2417.956 kb on - strandat 2418.020 kb on + strandat 2418.020 kb on + strandat 2418.021 kb on - strandat 2418.177 kb on + strand, within RR42_RS31385at 2418.264 kb on - strand, within RR42_RS31385at 2418.274 kb on - strand, within RR42_RS31385at 2418.312 kb on + strand, within RR42_RS31385at 2418.312 kb on + strand, within RR42_RS31385at 2418.437 kb on + strand, within RR42_RS31385at 2418.438 kb on - strand, within RR42_RS31385at 2418.438 kb on - strand, within RR42_RS31385at 2418.438 kb on - strand, within RR42_RS31385at 2418.576 kb on - strand, within RR42_RS31385at 2418.576 kb on - strand, within RR42_RS31385at 2418.614 kb on + strand, within RR42_RS31385at 2418.614 kb on + strand, within RR42_RS31385at 2418.615 kb on - strand, within RR42_RS31385at 2418.771 kb on + strandat 2418.771 kb on + strandat 2418.772 kb on - strandat 2418.772 kb on - strandat 2418.772 kb on - strandat 2418.860 kb on + strandat 2418.863 kb on - strandat 2418.867 kb on + strandat 2418.867 kb on + strandat 2418.868 kb on - strandat 2418.868 kb on - strandat 2418.870 kb on - strandat 2419.210 kb on + strand, within RR42_RS31390at 2419.210 kb on + strand, within RR42_RS31390at 2419.211 kb on - strand, within RR42_RS31390at 2419.242 kb on + strand, within RR42_RS31390at 2419.242 kb on + strand, within RR42_RS31390at 2419.243 kb on - strand, within RR42_RS31390at 2419.479 kb on - strand, within RR42_RS31390at 2419.589 kb on + strand, within RR42_RS31390at 2419.589 kb on + strand, within RR42_RS31390at 2419.589 kb on + strand, within RR42_RS31390at 2419.590 kb on - strand, within RR42_RS31390at 2419.595 kb on + strand, within RR42_RS31390at 2419.595 kb on + strand, within RR42_RS31390at 2419.595 kb on + strand, within RR42_RS31390at 2419.596 kb on - strand, within RR42_RS31390at 2419.703 kb on + strand, within RR42_RS31390at 2419.703 kb on + strand, within RR42_RS31390at 2419.704 kb on - strand, within RR42_RS31390at 2419.704 kb on - strand, within RR42_RS31390

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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2,417,006 + +2.2
2,417,006 + -0.4
2,417,006 + +0.7
2,417,006 + -0.2
2,417,007 - -1.6
2,417,115 - RR42_RS31380 0.10 -0.7
2,417,237 + RR42_RS31380 0.24 -0.4
2,417,237 + RR42_RS31380 0.24 -1.0
2,417,237 + RR42_RS31380 0.24 +0.9
2,417,237 + RR42_RS31380 0.24 -3.3
2,417,237 + RR42_RS31380 0.24 +0.5
2,417,237 + RR42_RS31380 0.24 -0.9
2,417,238 - RR42_RS31380 0.24 -0.3
2,417,238 - RR42_RS31380 0.24 -0.6
2,417,238 - RR42_RS31380 0.24 -0.8
2,417,361 - RR42_RS31380 0.38 -1.2
2,417,361 - RR42_RS31380 0.38 -0.4
2,417,446 + RR42_RS31380 0.48 -1.7
2,417,446 + RR42_RS31380 0.48 -1.0
2,417,498 + RR42_RS31380 0.54 +0.1
2,417,735 - RR42_RS31380 0.80 +0.1
2,417,806 + RR42_RS31380 0.88 -1.4
2,417,907 - +0.9
2,417,939 + +0.6
2,417,940 - -0.5
2,417,940 - +0.4
2,417,956 - +1.0
2,418,020 + -0.3
2,418,020 + -0.9
2,418,021 - +0.6
2,418,177 + RR42_RS31385 0.26 -0.7
2,418,264 - RR42_RS31385 0.38 -0.6
2,418,274 - RR42_RS31385 0.40 +0.1
2,418,312 + RR42_RS31385 0.45 -1.7
2,418,312 + RR42_RS31385 0.45 +1.1
2,418,437 + RR42_RS31385 0.62 -0.9
2,418,438 - RR42_RS31385 0.62 -0.7
2,418,438 - RR42_RS31385 0.62 +0.1
2,418,438 - RR42_RS31385 0.62 -0.7
2,418,576 - RR42_RS31385 0.82 -1.3
2,418,576 - RR42_RS31385 0.82 -0.0
2,418,614 + RR42_RS31385 0.87 -3.0
2,418,614 + RR42_RS31385 0.87 -0.1
2,418,615 - RR42_RS31385 0.87 -0.0
2,418,771 + -1.1
2,418,771 + -0.4
2,418,772 - -0.0
2,418,772 - -0.2
2,418,772 - +0.8
2,418,860 + -0.3
2,418,863 - -0.0
2,418,867 + -0.2
2,418,867 + +0.4
2,418,868 - -1.1
2,418,868 - +0.0
2,418,870 - -0.4
2,419,210 + RR42_RS31390 0.28 -0.1
2,419,210 + RR42_RS31390 0.28 -0.5
2,419,211 - RR42_RS31390 0.28 +0.3
2,419,242 + RR42_RS31390 0.31 +0.7
2,419,242 + RR42_RS31390 0.31 +0.7
2,419,243 - RR42_RS31390 0.31 -2.8
2,419,479 - RR42_RS31390 0.56 -1.1
2,419,589 + RR42_RS31390 0.67 -0.2
2,419,589 + RR42_RS31390 0.67 +0.6
2,419,589 + RR42_RS31390 0.67 -0.2
2,419,590 - RR42_RS31390 0.67 -0.7
2,419,595 + RR42_RS31390 0.67 +0.1
2,419,595 + RR42_RS31390 0.67 +1.8
2,419,595 + RR42_RS31390 0.67 +0.1
2,419,596 - RR42_RS31390 0.68 +0.9
2,419,703 + RR42_RS31390 0.79 +0.1
2,419,703 + RR42_RS31390 0.79 +1.1
2,419,704 - RR42_RS31390 0.79 +1.3
2,419,704 - RR42_RS31390 0.79 -0.6

Or see this region's nucleotide sequence