Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30700 and RR42_RS30705 are separated by 54 nucleotides RR42_RS30705 and RR42_RS30710 are separated by 309 nucleotides
RR42_RS30700: RR42_RS30700 - alpha/beta hydrolase, at 2,264,406 to 2,265,344
_RS30700
RR42_RS30705: RR42_RS30705 - FAD-containing monooxygenase EthA, at 2,265,399 to 2,266,859
_RS30705
RR42_RS30710: RR42_RS30710 - TetR family transcriptional regulator, at 2,267,169 to 2,267,777
_RS30710
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2264.471 kb on + strand at 2264.471 kb on + strand at 2264.570 kb on - strand, within RR42_RS30700 at 2264.602 kb on + strand, within RR42_RS30700 at 2264.662 kb on + strand, within RR42_RS30700 at 2264.662 kb on + strand, within RR42_RS30700 at 2264.663 kb on - strand, within RR42_RS30700 at 2264.663 kb on - strand, within RR42_RS30700 at 2264.663 kb on - strand, within RR42_RS30700 at 2264.663 kb on - strand, within RR42_RS30700 at 2264.663 kb on - strand, within RR42_RS30700 at 2264.760 kb on - strand, within RR42_RS30700 at 2264.771 kb on + strand, within RR42_RS30700 at 2264.771 kb on + strand, within RR42_RS30700 at 2264.771 kb on + strand, within RR42_RS30700 at 2264.771 kb on + strand, within RR42_RS30700 at 2264.772 kb on - strand, within RR42_RS30700 at 2264.772 kb on - strand, within RR42_RS30700 at 2264.772 kb on - strand, within RR42_RS30700 at 2264.772 kb on - strand, within RR42_RS30700 at 2264.817 kb on - strand, within RR42_RS30700 at 2264.985 kb on + strand, within RR42_RS30700 at 2265.015 kb on - strand, within RR42_RS30700 at 2265.015 kb on - strand, within RR42_RS30700 at 2265.127 kb on + strand, within RR42_RS30700 at 2265.128 kb on - strand, within RR42_RS30700 at 2265.128 kb on - strand, within RR42_RS30700 at 2265.199 kb on + strand, within RR42_RS30700 at 2265.199 kb on + strand, within RR42_RS30700 at 2265.199 kb on + strand, within RR42_RS30700 at 2265.199 kb on + strand, within RR42_RS30700 at 2265.200 kb on - strand, within RR42_RS30700 at 2265.336 kb on + strand at 2265.336 kb on + strand at 2265.336 kb on + strand at 2265.337 kb on - strand at 2265.337 kb on - strand at 2265.337 kb on - strand at 2265.337 kb on - strand at 2265.349 kb on + strand at 2265.349 kb on + strand at 2265.349 kb on + strand at 2265.349 kb on + strand at 2265.349 kb on + strand at 2265.350 kb on - strand at 2265.350 kb on - strand at 2265.350 kb on - strand at 2265.353 kb on + strand at 2265.354 kb on - strand at 2265.354 kb on - strand at 2265.494 kb on - strand at 2265.494 kb on - strand at 2265.494 kb on - strand at 2265.563 kb on - strand, within RR42_RS30705 at 2265.650 kb on + strand, within RR42_RS30705 at 2265.650 kb on + strand, within RR42_RS30705 at 2265.650 kb on + strand, within RR42_RS30705 at 2265.651 kb on - strand, within RR42_RS30705 at 2265.651 kb on - strand, within RR42_RS30705 at 2265.673 kb on + strand, within RR42_RS30705 at 2265.673 kb on + strand, within RR42_RS30705 at 2265.673 kb on + strand, within RR42_RS30705 at 2265.673 kb on + strand, within RR42_RS30705 at 2265.673 kb on + strand, within RR42_RS30705 at 2265.674 kb on - strand, within RR42_RS30705 at 2265.674 kb on - strand, within RR42_RS30705 at 2265.674 kb on - strand, within RR42_RS30705 at 2265.674 kb on - strand, within RR42_RS30705 at 2265.674 kb on - strand, within RR42_RS30705 at 2265.718 kb on + strand, within RR42_RS30705 at 2265.752 kb on + strand, within RR42_RS30705 at 2265.752 kb on + strand, within RR42_RS30705 at 2265.752 kb on + strand, within RR42_RS30705 at 2265.752 kb on + strand, within RR42_RS30705 at 2265.754 kb on + strand, within RR42_RS30705 at 2265.754 kb on + strand, within RR42_RS30705 at 2265.754 kb on + strand, within RR42_RS30705 at 2265.754 kb on + strand, within RR42_RS30705 at 2265.754 kb on + strand, within RR42_RS30705 at 2265.754 kb on + strand, within RR42_RS30705 at 2265.755 kb on - strand, within RR42_RS30705 at 2265.755 kb on - strand, within RR42_RS30705 at 2265.755 kb on - strand, within RR42_RS30705 at 2265.755 kb on - strand, within RR42_RS30705 at 2265.755 kb on - strand, within RR42_RS30705 at 2265.755 kb on - strand, within RR42_RS30705 at 2265.769 kb on + strand, within RR42_RS30705 at 2265.769 kb on + strand, within RR42_RS30705 at 2265.769 kb on + strand, within RR42_RS30705 at 2265.769 kb on + strand, within RR42_RS30705 at 2265.769 kb on + strand, within RR42_RS30705 at 2265.769 kb on + strand, within RR42_RS30705 at 2265.770 kb on - strand, within RR42_RS30705 at 2266.018 kb on + strand, within RR42_RS30705 at 2266.018 kb on + strand, within RR42_RS30705 at 2266.019 kb on - strand, within RR42_RS30705 at 2266.019 kb on - strand, within RR42_RS30705 at 2266.049 kb on - strand, within RR42_RS30705 at 2266.049 kb on - strand, within RR42_RS30705 at 2266.126 kb on + strand, within RR42_RS30705 at 2266.126 kb on + strand, within RR42_RS30705 at 2266.126 kb on + strand, within RR42_RS30705 at 2266.132 kb on + strand, within RR42_RS30705 at 2266.132 kb on + strand, within RR42_RS30705 at 2266.132 kb on + strand, within RR42_RS30705 at 2266.132 kb on + strand, within RR42_RS30705 at 2266.132 kb on + strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.133 kb on - strand, within RR42_RS30705 at 2266.243 kb on + strand, within RR42_RS30705 at 2266.243 kb on + strand, within RR42_RS30705 at 2266.243 kb on + strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.244 kb on - strand, within RR42_RS30705 at 2266.355 kb on - strand, within RR42_RS30705 at 2266.430 kb on + strand, within RR42_RS30705 at 2266.430 kb on + strand, within RR42_RS30705 at 2266.431 kb on - strand, within RR42_RS30705 at 2266.432 kb on + strand, within RR42_RS30705 at 2266.432 kb on + strand, within RR42_RS30705 at 2266.432 kb on + strand, within RR42_RS30705 at 2266.433 kb on - strand, within RR42_RS30705 at 2266.433 kb on - strand, within RR42_RS30705 at 2266.450 kb on + strand, within RR42_RS30705 at 2266.450 kb on + strand, within RR42_RS30705 at 2266.451 kb on - strand, within RR42_RS30705 at 2266.451 kb on - strand, within RR42_RS30705 at 2266.454 kb on - strand, within RR42_RS30705 at 2266.454 kb on - strand, within RR42_RS30705 at 2266.469 kb on - strand, within RR42_RS30705 at 2266.485 kb on - strand, within RR42_RS30705 at 2266.566 kb on + strand, within RR42_RS30705 at 2266.627 kb on + strand, within RR42_RS30705 at 2266.627 kb on + strand, within RR42_RS30705 at 2266.628 kb on - strand, within RR42_RS30705 at 2266.628 kb on - strand, within RR42_RS30705 at 2266.699 kb on + strand, within RR42_RS30705 at 2266.699 kb on + strand, within RR42_RS30705 at 2266.699 kb on + strand, within RR42_RS30705 at 2266.699 kb on + strand, within RR42_RS30705 at 2266.699 kb on + strand, within RR42_RS30705 at 2266.700 kb on - strand, within RR42_RS30705 at 2266.700 kb on - strand, within RR42_RS30705 at 2266.700 kb on - strand, within RR42_RS30705 at 2266.700 kb on - strand, within RR42_RS30705 at 2266.700 kb on - strand, within RR42_RS30705 at 2266.700 kb on - strand, within RR42_RS30705 at 2266.784 kb on - strand at 2266.784 kb on - strand at 2266.784 kb on - strand at 2266.784 kb on - strand at 2266.784 kb on - strand at 2266.816 kb on + strand at 2266.817 kb on - strand at 2266.852 kb on - strand at 2266.966 kb on - strand at 2266.966 kb on - strand at 2267.040 kb on + strand at 2267.040 kb on + strand at 2267.041 kb on - strand at 2267.041 kb on - strand at 2267.054 kb on + strand at 2267.054 kb on + strand at 2267.054 kb on + strand at 2267.055 kb on - strand at 2267.072 kb on + strand at 2267.102 kb on + strand at 2267.103 kb on - strand at 2267.103 kb on - strand at 2267.122 kb on - strand at 2267.135 kb on + strand at 2267.136 kb on - strand at 2267.143 kb on + strand at 2267.143 kb on + strand at 2267.155 kb on + strand at 2267.155 kb on + strand at 2267.156 kb on - strand at 2267.293 kb on + strand, within RR42_RS30710 at 2267.293 kb on + strand, within RR42_RS30710 at 2267.294 kb on - strand, within RR42_RS30710 at 2267.294 kb on - strand, within RR42_RS30710 at 2267.310 kb on + strand, within RR42_RS30710 at 2267.310 kb on + strand, within RR42_RS30710 at 2267.311 kb on - strand, within RR42_RS30710 at 2267.311 kb on - strand, within RR42_RS30710 at 2267.311 kb on - strand, within RR42_RS30710 at 2267.311 kb on - strand, within RR42_RS30710 at 2267.313 kb on + strand, within RR42_RS30710 at 2267.313 kb on + strand, within RR42_RS30710 at 2267.434 kb on - strand, within RR42_RS30710 at 2267.478 kb on + strand, within RR42_RS30710 at 2267.478 kb on + strand, within RR42_RS30710 at 2267.478 kb on + strand, within RR42_RS30710 at 2267.479 kb on - strand, within RR42_RS30710 at 2267.633 kb on - strand, within RR42_RS30710 at 2267.654 kb on - strand, within RR42_RS30710 at 2267.661 kb on + strand, within RR42_RS30710 at 2267.749 kb on + strand at 2267.750 kb on - strand at 2267.793 kb on - strand at 2267.858 kb on - strand at 2267.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,264,471 + -1.1 2,264,471 + +0.1 2,264,570 - RR42_RS30700 0.17 +0.1 2,264,602 + RR42_RS30700 0.21 +0.8 2,264,662 + RR42_RS30700 0.27 +0.5 2,264,662 + RR42_RS30700 0.27 -1.6 2,264,663 - RR42_RS30700 0.27 -1.0 2,264,663 - RR42_RS30700 0.27 -2.3 2,264,663 - RR42_RS30700 0.27 +0.2 2,264,663 - RR42_RS30700 0.27 -1.0 2,264,663 - RR42_RS30700 0.27 +0.3 2,264,760 - RR42_RS30700 0.38 -0.5 2,264,771 + RR42_RS30700 0.39 -0.4 2,264,771 + RR42_RS30700 0.39 -0.2 2,264,771 + RR42_RS30700 0.39 +1.2 2,264,771 + RR42_RS30700 0.39 +0.2 2,264,772 - RR42_RS30700 0.39 +1.3 2,264,772 - RR42_RS30700 0.39 -0.7 2,264,772 - RR42_RS30700 0.39 +0.3 2,264,772 - RR42_RS30700 0.39 +0.5 2,264,817 - RR42_RS30700 0.44 -0.3 2,264,985 + RR42_RS30700 0.62 -0.1 2,265,015 - RR42_RS30700 0.65 -0.9 2,265,015 - RR42_RS30700 0.65 -0.2 2,265,127 + RR42_RS30700 0.77 -0.8 2,265,128 - RR42_RS30700 0.77 +0.4 2,265,128 - RR42_RS30700 0.77 +0.4 2,265,199 + RR42_RS30700 0.84 -0.7 2,265,199 + RR42_RS30700 0.84 -0.3 2,265,199 + RR42_RS30700 0.84 -0.4 2,265,199 + RR42_RS30700 0.84 -0.0 2,265,200 - RR42_RS30700 0.85 -0.8 2,265,336 + +0.4 2,265,336 + +1.0 2,265,336 + -0.4 2,265,337 - +0.3 2,265,337 - +0.4 2,265,337 - -0.5 2,265,337 - +0.2 2,265,349 + -0.3 2,265,349 + -0.0 2,265,349 + -2.0 2,265,349 + -1.8 2,265,349 + +0.4 2,265,350 - +0.9 2,265,350 - -0.4 2,265,350 - +0.5 2,265,353 + -0.5 2,265,354 - +2.0 2,265,354 - +0.8 2,265,494 - +2.1 2,265,494 - +1.8 2,265,494 - -0.0 2,265,563 - RR42_RS30705 0.11 +1.8 2,265,650 + RR42_RS30705 0.17 +0.9 2,265,650 + RR42_RS30705 0.17 +0.5 2,265,650 + RR42_RS30705 0.17 +0.1 2,265,651 - RR42_RS30705 0.17 +2.4 2,265,651 - RR42_RS30705 0.17 +1.1 2,265,673 + RR42_RS30705 0.19 -0.6 2,265,673 + RR42_RS30705 0.19 +0.6 2,265,673 + RR42_RS30705 0.19 -0.0 2,265,673 + RR42_RS30705 0.19 -0.7 2,265,673 + RR42_RS30705 0.19 -0.4 2,265,674 - RR42_RS30705 0.19 +1.7 2,265,674 - RR42_RS30705 0.19 +1.4 2,265,674 - RR42_RS30705 0.19 +1.0 2,265,674 - RR42_RS30705 0.19 +0.1 2,265,674 - RR42_RS30705 0.19 +0.1 2,265,718 + RR42_RS30705 0.22 +0.8 2,265,752 + RR42_RS30705 0.24 +1.5 2,265,752 + RR42_RS30705 0.24 +0.8 2,265,752 + RR42_RS30705 0.24 -1.0 2,265,752 + RR42_RS30705 0.24 -0.3 2,265,754 + RR42_RS30705 0.24 +0.4 2,265,754 + RR42_RS30705 0.24 -0.8 2,265,754 + RR42_RS30705 0.24 -0.1 2,265,754 + RR42_RS30705 0.24 -1.9 2,265,754 + RR42_RS30705 0.24 -2.4 2,265,754 + RR42_RS30705 0.24 +0.1 2,265,755 - RR42_RS30705 0.24 +1.6 2,265,755 - RR42_RS30705 0.24 +1.7 2,265,755 - RR42_RS30705 0.24 +1.5 2,265,755 - RR42_RS30705 0.24 +1.9 2,265,755 - RR42_RS30705 0.24 -0.3 2,265,755 - RR42_RS30705 0.24 +2.2 2,265,769 + RR42_RS30705 0.25 -0.3 2,265,769 + RR42_RS30705 0.25 +0.1 2,265,769 + RR42_RS30705 0.25 +0.1 2,265,769 + RR42_RS30705 0.25 -1.0 2,265,769 + RR42_RS30705 0.25 +0.4 2,265,769 + RR42_RS30705 0.25 -0.2 2,265,770 - RR42_RS30705 0.25 +1.2 2,266,018 + RR42_RS30705 0.42 -2.3 2,266,018 + RR42_RS30705 0.42 +1.3 2,266,019 - RR42_RS30705 0.42 +2.4 2,266,019 - RR42_RS30705 0.42 +1.9 2,266,049 - RR42_RS30705 0.44 +0.6 2,266,049 - RR42_RS30705 0.44 +2.2 2,266,126 + RR42_RS30705 0.50 +0.1 2,266,126 + RR42_RS30705 0.50 -0.0 2,266,126 + RR42_RS30705 0.50 +1.9 2,266,132 + RR42_RS30705 0.50 +1.4 2,266,132 + RR42_RS30705 0.50 +0.4 2,266,132 + RR42_RS30705 0.50 +1.0 2,266,132 + RR42_RS30705 0.50 -0.4 2,266,132 + RR42_RS30705 0.50 +0.6 2,266,133 - RR42_RS30705 0.50 +1.6 2,266,133 - RR42_RS30705 0.50 +1.7 2,266,133 - RR42_RS30705 0.50 +1.8 2,266,133 - RR42_RS30705 0.50 +1.2 2,266,133 - RR42_RS30705 0.50 +1.4 2,266,133 - RR42_RS30705 0.50 +0.6 2,266,133 - RR42_RS30705 0.50 +1.8 2,266,133 - RR42_RS30705 0.50 +1.6 2,266,133 - RR42_RS30705 0.50 +2.1 2,266,243 + RR42_RS30705 0.58 +1.0 2,266,243 + RR42_RS30705 0.58 -0.9 2,266,243 + RR42_RS30705 0.58 -0.8 2,266,244 - RR42_RS30705 0.58 +0.4 2,266,244 - RR42_RS30705 0.58 +1.7 2,266,244 - RR42_RS30705 0.58 +1.9 2,266,244 - RR42_RS30705 0.58 +1.3 2,266,244 - RR42_RS30705 0.58 +0.6 2,266,244 - RR42_RS30705 0.58 +2.7 2,266,244 - RR42_RS30705 0.58 +1.6 2,266,244 - RR42_RS30705 0.58 +1.1 2,266,244 - RR42_RS30705 0.58 +1.5 2,266,355 - RR42_RS30705 0.65 -1.2 2,266,430 + RR42_RS30705 0.71 +0.8 2,266,430 + RR42_RS30705 0.71 +0.3 2,266,431 - RR42_RS30705 0.71 +1.8 2,266,432 + RR42_RS30705 0.71 +0.0 2,266,432 + RR42_RS30705 0.71 +0.8 2,266,432 + RR42_RS30705 0.71 +0.6 2,266,433 - RR42_RS30705 0.71 +1.1 2,266,433 - RR42_RS30705 0.71 +0.6 2,266,450 + RR42_RS30705 0.72 -0.2 2,266,450 + RR42_RS30705 0.72 -1.0 2,266,451 - RR42_RS30705 0.72 +2.1 2,266,451 - RR42_RS30705 0.72 +2.3 2,266,454 - RR42_RS30705 0.72 +3.3 2,266,454 - RR42_RS30705 0.72 +0.6 2,266,469 - RR42_RS30705 0.73 +1.2 2,266,485 - RR42_RS30705 0.74 +0.6 2,266,566 + RR42_RS30705 0.80 -0.0 2,266,627 + RR42_RS30705 0.84 -0.4 2,266,627 + RR42_RS30705 0.84 -1.7 2,266,628 - RR42_RS30705 0.84 +1.2 2,266,628 - RR42_RS30705 0.84 +1.4 2,266,699 + RR42_RS30705 0.89 -3.6 2,266,699 + RR42_RS30705 0.89 -0.2 2,266,699 + RR42_RS30705 0.89 +0.5 2,266,699 + RR42_RS30705 0.89 +0.9 2,266,699 + RR42_RS30705 0.89 +0.5 2,266,700 - RR42_RS30705 0.89 +0.8 2,266,700 - RR42_RS30705 0.89 +1.6 2,266,700 - RR42_RS30705 0.89 +0.5 2,266,700 - RR42_RS30705 0.89 -0.5 2,266,700 - RR42_RS30705 0.89 -0.3 2,266,700 - RR42_RS30705 0.89 +0.9 2,266,784 - +0.3 2,266,784 - +0.4 2,266,784 - -0.3 2,266,784 - +0.8 2,266,784 - +1.3 2,266,816 + +0.6 2,266,817 - +0.5 2,266,852 - -1.2 2,266,966 - -0.0 2,266,966 - +0.5 2,267,040 + -0.2 2,267,040 + -0.5 2,267,041 - +0.4 2,267,041 - +0.6 2,267,054 + -3.3 2,267,054 + -0.2 2,267,054 + -1.4 2,267,055 - +1.3 2,267,072 + -0.1 2,267,102 + -0.3 2,267,103 - +2.1 2,267,103 - +1.9 2,267,122 - +1.9 2,267,135 + +1.0 2,267,136 - +2.0 2,267,143 + +0.7 2,267,143 + -0.3 2,267,155 + -3.3 2,267,155 + -0.7 2,267,156 - +2.0 2,267,293 + RR42_RS30710 0.20 +1.3 2,267,293 + RR42_RS30710 0.20 +1.6 2,267,294 - RR42_RS30710 0.21 +1.1 2,267,294 - RR42_RS30710 0.21 +2.3 2,267,310 + RR42_RS30710 0.23 -0.1 2,267,310 + RR42_RS30710 0.23 +2.5 2,267,311 - RR42_RS30710 0.23 +1.8 2,267,311 - RR42_RS30710 0.23 +1.3 2,267,311 - RR42_RS30710 0.23 +0.7 2,267,311 - RR42_RS30710 0.23 +2.1 2,267,313 + RR42_RS30710 0.24 +1.2 2,267,313 + RR42_RS30710 0.24 -0.3 2,267,434 - RR42_RS30710 0.44 +0.9 2,267,478 + RR42_RS30710 0.51 +0.8 2,267,478 + RR42_RS30710 0.51 +0.6 2,267,478 + RR42_RS30710 0.51 +0.2 2,267,479 - RR42_RS30710 0.51 +1.5 2,267,633 - RR42_RS30710 0.76 +1.1 2,267,654 - RR42_RS30710 0.80 +2.5 2,267,661 + RR42_RS30710 0.81 -0.1 2,267,749 + -0.0 2,267,750 - +1.0 2,267,793 - -1.4 2,267,858 - +0.6 2,267,858 - +0.5
Or see this region's nucleotide sequence