Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30160 and RR42_RS30165 are separated by 178 nucleotides RR42_RS30165 and RR42_RS30170 are separated by 123 nucleotides
RR42_RS30160: RR42_RS30160 - sulfatase, at 2,140,146 to 2,141,345
_RS30160
RR42_RS30165: RR42_RS30165 - membrane protein, at 2,141,524 to 2,142,720
_RS30165
RR42_RS30170: RR42_RS30170 - aldolase, at 2,142,844 to 2,143,608
_RS30170
Position (kb)
2141
2142
2143 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.748 kb on - strand, within RR42_RS30160 at 2140.871 kb on - strand, within RR42_RS30160 at 2141.014 kb on + strand, within RR42_RS30160 at 2141.014 kb on + strand, within RR42_RS30160 at 2141.015 kb on - strand, within RR42_RS30160 at 2141.454 kb on - strand at 2141.454 kb on - strand at 2141.507 kb on + strand at 2141.508 kb on - strand at 2141.508 kb on - strand at 2141.526 kb on - strand at 2141.581 kb on + strand at 2141.582 kb on - strand at 2141.645 kb on + strand, within RR42_RS30165 at 2141.646 kb on - strand, within RR42_RS30165 at 2141.646 kb on - strand, within RR42_RS30165 at 2141.660 kb on + strand, within RR42_RS30165 at 2141.660 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.778 kb on - strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.030 kb on - strand, within RR42_RS30165 at 2142.054 kb on + strand, within RR42_RS30165 at 2142.055 kb on - strand, within RR42_RS30165 at 2142.143 kb on + strand, within RR42_RS30165 at 2142.144 kb on - strand, within RR42_RS30165 at 2142.182 kb on + strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.512 kb on - strand, within RR42_RS30165 at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.730 kb on + strand at 2142.730 kb on + strand at 2142.733 kb on + strand at 2142.734 kb on - strand at 2142.734 kb on - strand at 2142.845 kb on + strand at 2142.846 kb on - strand at 2142.846 kb on - strand at 2143.034 kb on + strand, within RR42_RS30170 at 2143.034 kb on + strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.266 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.600 kb on - strand at 2143.630 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,140,666 + RR42_RS30160 0.43 -1.2 2,140,666 + RR42_RS30160 0.43 +0.2 2,140,666 + RR42_RS30160 0.43 -0.1 2,140,666 + RR42_RS30160 0.43 +0.0 2,140,667 - RR42_RS30160 0.43 -0.2 2,140,667 - RR42_RS30160 0.43 +0.4 2,140,667 - RR42_RS30160 0.43 +0.5 2,140,748 - RR42_RS30160 0.50 +0.1 2,140,871 - RR42_RS30160 0.60 -0.2 2,141,014 + RR42_RS30160 0.72 -0.7 2,141,014 + RR42_RS30160 0.72 -0.4 2,141,015 - RR42_RS30160 0.72 +0.4 2,141,454 - -0.2 2,141,454 - +0.7 2,141,507 + +0.7 2,141,508 - -0.4 2,141,508 - -0.2 2,141,526 - -0.1 2,141,581 + +0.5 2,141,582 - -0.5 2,141,645 + RR42_RS30165 0.10 -1.8 2,141,646 - RR42_RS30165 0.10 +0.3 2,141,646 - RR42_RS30165 0.10 +0.2 2,141,660 + RR42_RS30165 0.11 -0.1 2,141,660 + RR42_RS30165 0.11 -0.7 2,141,774 + RR42_RS30165 0.21 -0.1 2,141,774 + RR42_RS30165 0.21 -1.4 2,141,774 + RR42_RS30165 0.21 +0.0 2,141,775 - RR42_RS30165 0.21 -0.7 2,141,775 - RR42_RS30165 0.21 +0.9 2,141,775 - RR42_RS30165 0.21 +0.7 2,141,775 - RR42_RS30165 0.21 +0.1 2,141,778 - RR42_RS30165 0.21 +0.4 2,141,924 + RR42_RS30165 0.33 -0.5 2,141,924 + RR42_RS30165 0.33 -1.1 2,141,925 - RR42_RS30165 0.34 -0.5 2,141,925 - RR42_RS30165 0.34 -0.2 2,142,029 + RR42_RS30165 0.42 -1.5 2,142,029 + RR42_RS30165 0.42 +0.4 2,142,030 - RR42_RS30165 0.42 -0.7 2,142,054 + RR42_RS30165 0.44 -1.5 2,142,055 - RR42_RS30165 0.44 +0.6 2,142,143 + RR42_RS30165 0.52 +0.2 2,142,144 - RR42_RS30165 0.52 +0.9 2,142,182 + RR42_RS30165 0.55 -0.7 2,142,294 - RR42_RS30165 0.64 -0.2 2,142,294 - RR42_RS30165 0.64 -0.6 2,142,294 - RR42_RS30165 0.64 +1.1 2,142,294 - RR42_RS30165 0.64 -0.1 2,142,512 - RR42_RS30165 0.83 -0.4 2,142,626 + -0.4 2,142,626 + +0.0 2,142,626 + -0.8 2,142,627 - -0.2 2,142,627 - -0.4 2,142,627 - -0.1 2,142,627 - -0.2 2,142,705 - +0.0 2,142,705 - +0.3 2,142,705 - +0.1 2,142,709 - +0.3 2,142,709 - +0.2 2,142,709 - -0.2 2,142,730 + +0.7 2,142,730 + -0.1 2,142,733 + +0.4 2,142,734 - -3.0 2,142,734 - -0.5 2,142,845 + +1.1 2,142,846 - +0.7 2,142,846 - -3.7 2,143,034 + RR42_RS30170 0.25 +0.2 2,143,034 + RR42_RS30170 0.25 +0.1 2,143,035 - RR42_RS30170 0.25 +0.1 2,143,035 - RR42_RS30170 0.25 -1.3 2,143,035 - RR42_RS30170 0.25 -0.6 2,143,266 + RR42_RS30170 0.55 +0.2 2,143,272 + RR42_RS30170 0.56 +2.5 2,143,272 + RR42_RS30170 0.56 +1.2 2,143,600 - +1.3 2,143,630 + -0.3
Or see this region's nucleotide sequence