Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30090 and RR42_RS30095 are separated by 183 nucleotides RR42_RS30095 and RR42_RS30100 are separated by 73 nucleotides
RR42_RS30090: RR42_RS30090 - MFS transporter, at 2,122,565 to 2,123,836
_RS30090
RR42_RS30095: RR42_RS30095 - alcohol dehydrogenase, at 2,124,020 to 2,124,991
_RS30095
RR42_RS30100: RR42_RS30100 - transposase, at 2,125,065 to 2,126,123
_RS30100
Position (kb)
2124
2125 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2123.062 kb on - strand, within RR42_RS30090 at 2123.233 kb on - strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.384 kb on + strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.385 kb on - strand, within RR42_RS30090 at 2123.489 kb on + strand, within RR42_RS30090 at 2123.489 kb on + strand, within RR42_RS30090 at 2123.499 kb on + strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.500 kb on - strand, within RR42_RS30090 at 2123.525 kb on - strand, within RR42_RS30090 at 2123.531 kb on + strand, within RR42_RS30090 at 2123.900 kb on + strand at 2123.900 kb on + strand at 2123.901 kb on - strand at 2124.044 kb on + strand at 2124.190 kb on + strand, within RR42_RS30095 at 2124.191 kb on - strand, within RR42_RS30095 at 2124.192 kb on + strand, within RR42_RS30095 at 2124.192 kb on + strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.193 kb on - strand, within RR42_RS30095 at 2124.559 kb on - strand, within RR42_RS30095 at 2124.606 kb on + strand, within RR42_RS30095 at 2124.606 kb on + strand, within RR42_RS30095 at 2124.735 kb on + strand, within RR42_RS30095 at 2124.838 kb on - strand, within RR42_RS30095 at 2124.855 kb on + strand, within RR42_RS30095 at 2124.856 kb on - strand, within RR42_RS30095 at 2124.856 kb on - strand, within RR42_RS30095 at 2124.859 kb on + strand, within RR42_RS30095 at 2124.882 kb on + strand, within RR42_RS30095 at 2124.882 kb on + strand, within RR42_RS30095 at 2124.882 kb on + strand, within RR42_RS30095 at 2124.883 kb on - strand, within RR42_RS30095 at 2124.924 kb on - strand at 2125.007 kb on + strand at 2125.007 kb on + strand at 2125.007 kb on + strand at 2125.008 kb on - strand at 2125.008 kb on - strand at 2125.008 kb on - strand at 2125.426 kb on + strand, within RR42_RS30100 at 2125.489 kb on + strand, within RR42_RS30100 at 2125.489 kb on + strand, within RR42_RS30100 at 2125.489 kb on + strand, within RR42_RS30100 at 2125.490 kb on - strand, within RR42_RS30100 at 2125.541 kb on - strand, within RR42_RS30100 at 2125.633 kb on + strand, within RR42_RS30100 at 2125.633 kb on + strand, within RR42_RS30100 at 2125.633 kb on + strand, within RR42_RS30100 at 2125.685 kb on - strand, within RR42_RS30100 at 2125.972 kb on + strand, within RR42_RS30100 at 2125.972 kb on + strand, within RR42_RS30100 at 2125.972 kb on + strand, within RR42_RS30100 at 2125.973 kb on - strand, within RR42_RS30100
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,123,062 - RR42_RS30090 0.39 -1.2 2,123,233 - RR42_RS30090 0.53 +0.5 2,123,384 + RR42_RS30090 0.64 +0.5 2,123,384 + RR42_RS30090 0.64 +0.8 2,123,384 + RR42_RS30090 0.64 +0.5 2,123,384 + RR42_RS30090 0.64 +0.3 2,123,385 - RR42_RS30090 0.64 -0.2 2,123,385 - RR42_RS30090 0.64 +1.2 2,123,385 - RR42_RS30090 0.64 -0.1 2,123,385 - RR42_RS30090 0.64 -0.2 2,123,489 + RR42_RS30090 0.73 +0.3 2,123,489 + RR42_RS30090 0.73 -0.5 2,123,499 + RR42_RS30090 0.73 -0.1 2,123,500 - RR42_RS30090 0.74 +0.2 2,123,500 - RR42_RS30090 0.74 -0.7 2,123,500 - RR42_RS30090 0.74 +1.1 2,123,525 - RR42_RS30090 0.75 -0.0 2,123,531 + RR42_RS30090 0.76 -0.0 2,123,900 + -3.3 2,123,900 + +0.5 2,123,901 - -0.2 2,124,044 + +0.5 2,124,190 + RR42_RS30095 0.17 -0.3 2,124,191 - RR42_RS30095 0.18 +0.9 2,124,192 + RR42_RS30095 0.18 +0.5 2,124,192 + RR42_RS30095 0.18 +0.4 2,124,193 - RR42_RS30095 0.18 -1.1 2,124,193 - RR42_RS30095 0.18 +0.3 2,124,193 - RR42_RS30095 0.18 +0.3 2,124,193 - RR42_RS30095 0.18 +0.1 2,124,559 - RR42_RS30095 0.55 -0.5 2,124,606 + RR42_RS30095 0.60 +1.4 2,124,606 + RR42_RS30095 0.60 +0.9 2,124,735 + RR42_RS30095 0.74 -0.1 2,124,838 - RR42_RS30095 0.84 +1.4 2,124,855 + RR42_RS30095 0.86 -0.3 2,124,856 - RR42_RS30095 0.86 +1.6 2,124,856 - RR42_RS30095 0.86 -0.7 2,124,859 + RR42_RS30095 0.86 +0.6 2,124,882 + RR42_RS30095 0.89 +0.1 2,124,882 + RR42_RS30095 0.89 +1.1 2,124,882 + RR42_RS30095 0.89 +1.1 2,124,883 - RR42_RS30095 0.89 -1.7 2,124,924 - +0.7 2,125,007 + +0.5 2,125,007 + +0.8 2,125,007 + -3.6 2,125,008 - -0.2 2,125,008 - -0.7 2,125,008 - -0.2 2,125,426 + RR42_RS30100 0.34 +0.7 2,125,489 + RR42_RS30100 0.40 -0.5 2,125,489 + RR42_RS30100 0.40 +0.3 2,125,489 + RR42_RS30100 0.40 -0.7 2,125,490 - RR42_RS30100 0.40 +0.4 2,125,541 - RR42_RS30100 0.45 +0.5 2,125,633 + RR42_RS30100 0.54 +0.2 2,125,633 + RR42_RS30100 0.54 -0.1 2,125,633 + RR42_RS30100 0.54 -0.2 2,125,685 - RR42_RS30100 0.59 -0.2 2,125,972 + RR42_RS30100 0.86 +0.6 2,125,972 + RR42_RS30100 0.86 -1.2 2,125,972 + RR42_RS30100 0.86 -0.4 2,125,973 - RR42_RS30100 0.86 +1.3
Or see this region's nucleotide sequence