Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29335 and RR42_RS29340 are separated by 308 nucleotides RR42_RS29340 and RR42_RS29345 are separated by 208 nucleotides RR42_RS29345 and RR42_RS29350 are separated by 79 nucleotides
RR42_RS29335: RR42_RS29335 - integrase, at 1,935,039 to 1,935,983
_RS29335
RR42_RS29340: RR42_RS29340 - hypothetical protein, at 1,936,292 to 1,937,260
_RS29340
RR42_RS29345: RR42_RS29345 - ArsR family transcriptional regulator, at 1,937,469 to 1,937,834
_RS29345
RR42_RS29350: RR42_RS29350 - glycerol uptake facilitator or water-selective channels, at 1,937,914 to 1,938,639
_RS29350
Position (kb)
1936
1937
1938 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1935.780 kb on - strand, within RR42_RS29335 at 1935.873 kb on + strand, within RR42_RS29335 at 1935.873 kb on + strand, within RR42_RS29335 at 1936.147 kb on - strand at 1936.147 kb on - strand at 1936.394 kb on - strand, within RR42_RS29340 at 1936.550 kb on + strand, within RR42_RS29340 at 1936.550 kb on + strand, within RR42_RS29340 at 1936.551 kb on - strand, within RR42_RS29340 at 1936.551 kb on - strand, within RR42_RS29340 at 1936.551 kb on - strand, within RR42_RS29340 at 1936.601 kb on + strand, within RR42_RS29340 at 1936.602 kb on - strand, within RR42_RS29340 at 1936.602 kb on - strand, within RR42_RS29340 at 1936.602 kb on - strand, within RR42_RS29340 at 1937.182 kb on + strand at 1937.259 kb on - strand at 1937.259 kb on - strand at 1937.259 kb on - strand at 1937.259 kb on - strand at 1937.259 kb on - strand at 1937.259 kb on - strand at 1937.259 kb on - strand at 1937.293 kb on + strand at 1937.293 kb on + strand at 1937.294 kb on - strand at 1937.294 kb on - strand at 1937.294 kb on - strand at 1937.407 kb on + strand at 1937.436 kb on + strand at 1937.436 kb on + strand at 1937.436 kb on + strand at 1937.437 kb on - strand at 1937.437 kb on - strand at 1937.685 kb on + strand, within RR42_RS29345 at 1937.686 kb on - strand, within RR42_RS29345 at 1937.686 kb on - strand, within RR42_RS29345 at 1937.686 kb on - strand, within RR42_RS29345 at 1937.719 kb on + strand, within RR42_RS29345 at 1937.719 kb on + strand, within RR42_RS29345 at 1937.719 kb on + strand, within RR42_RS29345 at 1937.720 kb on - strand, within RR42_RS29345 at 1938.012 kb on + strand, within RR42_RS29350 at 1938.097 kb on - strand, within RR42_RS29350 at 1938.097 kb on - strand, within RR42_RS29350 at 1938.132 kb on + strand, within RR42_RS29350 at 1938.162 kb on - strand, within RR42_RS29350 at 1938.162 kb on - strand, within RR42_RS29350 at 1938.162 kb on - strand, within RR42_RS29350 at 1938.172 kb on + strand, within RR42_RS29350 at 1938.176 kb on + strand, within RR42_RS29350 at 1938.176 kb on + strand, within RR42_RS29350 at 1938.176 kb on + strand, within RR42_RS29350 at 1938.177 kb on + strand, within RR42_RS29350 at 1938.177 kb on - strand, within RR42_RS29350 at 1938.177 kb on - strand, within RR42_RS29350 at 1938.177 kb on - strand, within RR42_RS29350 at 1938.229 kb on + strand, within RR42_RS29350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 1,935,780 - RR42_RS29335 0.78 +2.1 1,935,873 + RR42_RS29335 0.88 -1.2 1,935,873 + RR42_RS29335 0.88 -0.6 1,936,147 - +0.8 1,936,147 - +1.6 1,936,394 - RR42_RS29340 0.11 +1.0 1,936,550 + RR42_RS29340 0.27 +0.3 1,936,550 + RR42_RS29340 0.27 +2.0 1,936,551 - RR42_RS29340 0.27 +2.1 1,936,551 - RR42_RS29340 0.27 +1.1 1,936,551 - RR42_RS29340 0.27 +0.2 1,936,601 + RR42_RS29340 0.32 +2.0 1,936,602 - RR42_RS29340 0.32 +1.0 1,936,602 - RR42_RS29340 0.32 +0.4 1,936,602 - RR42_RS29340 0.32 +0.1 1,937,182 + +0.7 1,937,259 - -1.0 1,937,259 - -0.5 1,937,259 - +1.1 1,937,259 - -1.9 1,937,259 - -1.0 1,937,259 - -0.3 1,937,259 - -0.2 1,937,293 + -2.6 1,937,293 + +0.0 1,937,294 - -0.5 1,937,294 - -0.7 1,937,294 - +0.5 1,937,407 + +1.6 1,937,436 + -0.8 1,937,436 + -0.1 1,937,436 + -0.2 1,937,437 - +1.2 1,937,437 - -0.3 1,937,685 + RR42_RS29345 0.59 -0.1 1,937,686 - RR42_RS29345 0.59 -0.3 1,937,686 - RR42_RS29345 0.59 +0.3 1,937,686 - RR42_RS29345 0.59 -0.0 1,937,719 + RR42_RS29345 0.68 -2.5 1,937,719 + RR42_RS29345 0.68 +0.0 1,937,719 + RR42_RS29345 0.68 +0.1 1,937,720 - RR42_RS29345 0.69 +2.0 1,938,012 + RR42_RS29350 0.13 -0.3 1,938,097 - RR42_RS29350 0.25 +0.4 1,938,097 - RR42_RS29350 0.25 +0.9 1,938,132 + RR42_RS29350 0.30 +0.6 1,938,162 - RR42_RS29350 0.34 +0.7 1,938,162 - RR42_RS29350 0.34 -0.6 1,938,162 - RR42_RS29350 0.34 +0.3 1,938,172 + RR42_RS29350 0.36 +0.9 1,938,176 + RR42_RS29350 0.36 +0.9 1,938,176 + RR42_RS29350 0.36 +0.1 1,938,176 + RR42_RS29350 0.36 -0.6 1,938,177 + RR42_RS29350 0.36 +1.1 1,938,177 - RR42_RS29350 0.36 +0.1 1,938,177 - RR42_RS29350 0.36 +1.0 1,938,177 - RR42_RS29350 0.36 +0.3 1,938,229 + RR42_RS29350 0.43 +0.3
Or see this region's nucleotide sequence