Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28555 and RR42_RS28560 are separated by 449 nucleotides RR42_RS28560 and RR42_RS28565 are separated by 76 nucleotides
RR42_RS28555: RR42_RS28555 - 3-hydroxyisobutyrate dehydrogenase, at 1,761,093 to 1,761,992
_RS28555
RR42_RS28560: RR42_RS28560 - methylmalonate-semialdehyde dehydrogenase, at 1,762,442 to 1,763,962
_RS28560
RR42_RS28565: RR42_RS28565 - acyl-CoA dehydrogenase, at 1,764,039 to 1,765,172
_RS28565
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1761.970 kb on - strand at 1761.984 kb on + strand at 1761.984 kb on + strand at 1761.984 kb on + strand at 1761.985 kb on - strand at 1762.042 kb on + strand at 1762.043 kb on - strand at 1762.462 kb on + strand at 1762.527 kb on + strand at 1762.551 kb on + strand at 1762.698 kb on - strand, within RR42_RS28560 at 1762.772 kb on + strand, within RR42_RS28560 at 1762.773 kb on - strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.979 kb on + strand, within RR42_RS28560 at 1762.980 kb on - strand, within RR42_RS28560 at 1763.067 kb on - strand, within RR42_RS28560 at 1763.067 kb on - strand, within RR42_RS28560 at 1763.236 kb on - strand, within RR42_RS28560 at 1763.236 kb on - strand, within RR42_RS28560 at 1763.535 kb on + strand, within RR42_RS28560 at 1763.539 kb on + strand, within RR42_RS28560 at 1763.681 kb on - strand, within RR42_RS28560 at 1763.721 kb on + strand, within RR42_RS28560 at 1763.721 kb on + strand, within RR42_RS28560 at 1763.983 kb on + strand at 1763.983 kb on + strand at 1764.088 kb on + strand at 1764.134 kb on - strand at 1764.145 kb on + strand at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.845 kb on - strand, within RR42_RS28565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 1,761,970 - -0.3 1,761,984 + -0.9 1,761,984 + +0.9 1,761,984 + +0.6 1,761,985 - +0.8 1,762,042 + +0.2 1,762,043 - -1.3 1,762,462 + +0.1 1,762,527 + +0.3 1,762,551 + +0.8 1,762,698 - RR42_RS28560 0.17 +0.7 1,762,772 + RR42_RS28560 0.22 +0.1 1,762,773 - RR42_RS28560 0.22 -1.9 1,762,803 + RR42_RS28560 0.24 -2.5 1,762,803 + RR42_RS28560 0.24 -0.1 1,762,803 + RR42_RS28560 0.24 +0.3 1,762,979 + RR42_RS28560 0.35 +0.5 1,762,980 - RR42_RS28560 0.35 +1.5 1,763,067 - RR42_RS28560 0.41 +0.5 1,763,067 - RR42_RS28560 0.41 -0.7 1,763,236 - RR42_RS28560 0.52 +0.5 1,763,236 - RR42_RS28560 0.52 +0.5 1,763,535 + RR42_RS28560 0.72 +0.9 1,763,539 + RR42_RS28560 0.72 +1.2 1,763,681 - RR42_RS28560 0.81 -1.5 1,763,721 + RR42_RS28560 0.84 +0.2 1,763,721 + RR42_RS28560 0.84 -4.4 1,763,983 + -0.2 1,763,983 + +0.5 1,764,088 + +1.5 1,764,134 - -0.4 1,764,145 + +0.1 1,764,209 - RR42_RS28565 0.15 +0.1 1,764,209 - RR42_RS28565 0.15 -0.6 1,764,209 - RR42_RS28565 0.15 -0.7 1,764,209 - RR42_RS28565 0.15 +0.7 1,764,209 - RR42_RS28565 0.15 -2.4 1,764,845 - RR42_RS28565 0.71 +0.5
Or see this region's nucleotide sequence