Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS28305

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS28300 and RR42_RS28305 are separated by 474 nucleotides RR42_RS28300: RR42_RS28300 - L-threonine 3-dehydrogenase (EC 1.1.1.103) (from data), at 1,709,449 to 1,710,423 _RS28300 RR42_RS28305: RR42_RS28305 - L-threonine:H+ symporter (from data), at 1,710,898 to 1,712,316 _RS28305 Position (kb) 1710 1711 1712 1713Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1710.049 kb on + strand, within RR42_RS28300at 1710.049 kb on + strand, within RR42_RS28300at 1710.050 kb on - strand, within RR42_RS28300at 1710.050 kb on - strand, within RR42_RS28300at 1710.050 kb on - strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.092 kb on - strand, within RR42_RS28300at 1710.092 kb on - strand, within RR42_RS28300at 1710.167 kb on - strand, within RR42_RS28300at 1710.248 kb on - strand, within RR42_RS28300at 1710.482 kb on + strandat 1710.483 kb on - strandat 1710.529 kb on - strandat 1710.530 kb on + strandat 1710.530 kb on + strandat 1710.531 kb on - strandat 1710.531 kb on - strandat 1710.553 kb on + strandat 1710.638 kb on + strandat 1710.675 kb on + strandat 1710.676 kb on - strandat 1710.676 kb on - strandat 1710.898 kb on - strandat 1710.898 kb on - strandat 1710.898 kb on - strandat 1710.909 kb on - strandat 1711.075 kb on + strand, within RR42_RS28305at 1711.075 kb on + strand, within RR42_RS28305at 1711.136 kb on - strand, within RR42_RS28305at 1711.165 kb on + strand, within RR42_RS28305at 1711.270 kb on + strand, within RR42_RS28305at 1711.271 kb on - strand, within RR42_RS28305at 1711.337 kb on - strand, within RR42_RS28305at 1711.672 kb on + strand, within RR42_RS28305at 1711.747 kb on + strand, within RR42_RS28305at 1711.862 kb on - strand, within RR42_RS28305at 1711.862 kb on - strand, within RR42_RS28305at 1711.981 kb on + strand, within RR42_RS28305at 1711.981 kb on + strand, within RR42_RS28305at 1712.017 kb on - strand, within RR42_RS28305at 1712.017 kb on - strand, within RR42_RS28305at 1712.017 kb on - strand, within RR42_RS28305at 1712.141 kb on - strand, within RR42_RS28305at 1712.221 kb on + strandat 1712.221 kb on + strandat 1712.221 kb on + strandat 1712.613 kb on + strandat 1712.617 kb on + strandat 1712.618 kb on - strandat 1712.618 kb on - strandat 1712.848 kb on + strandat 1712.889 kb on + strandat 1713.010 kb on - strandat 1713.097 kb on + strandat 1713.099 kb on + strandat 1713.100 kb on - strandat 1713.112 kb on - strandat 1713.124 kb on - strandat 1713.124 kb on - strandat 1713.185 kb on + strandat 1713.186 kb on - strandat 1713.186 kb on - strandat 1713.193 kb on + strandat 1713.193 kb on + strandat 1713.307 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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1,710,049 + RR42_RS28300 0.62 -0.2
1,710,049 + RR42_RS28300 0.62 -1.5
1,710,050 - RR42_RS28300 0.62 +1.1
1,710,050 - RR42_RS28300 0.62 -0.6
1,710,050 - RR42_RS28300 0.62 +1.0
1,710,091 + RR42_RS28300 0.66 +0.5
1,710,091 + RR42_RS28300 0.66 -0.5
1,710,091 + RR42_RS28300 0.66 -1.7
1,710,091 + RR42_RS28300 0.66 -1.0
1,710,092 - RR42_RS28300 0.66 -0.1
1,710,092 - RR42_RS28300 0.66 -0.1
1,710,167 - RR42_RS28300 0.74 -1.0
1,710,248 - RR42_RS28300 0.82 +0.3
1,710,482 + -2.7
1,710,483 - -0.2
1,710,529 - +0.5
1,710,530 + -1.3
1,710,530 + +0.7
1,710,531 - +0.4
1,710,531 - -0.4
1,710,553 + -1.6
1,710,638 + -0.3
1,710,675 + -1.0
1,710,676 - +1.1
1,710,676 - +0.3
1,710,898 - -0.3
1,710,898 - +2.8
1,710,898 - +1.9
1,710,909 - +0.5
1,711,075 + RR42_RS28305 0.12 -0.4
1,711,075 + RR42_RS28305 0.12 -0.6
1,711,136 - RR42_RS28305 0.17 +1.2
1,711,165 + RR42_RS28305 0.19 -1.7
1,711,270 + RR42_RS28305 0.26 -0.6
1,711,271 - RR42_RS28305 0.26 +1.0
1,711,337 - RR42_RS28305 0.31 -0.1
1,711,672 + RR42_RS28305 0.55 -1.3
1,711,747 + RR42_RS28305 0.60 +0.2
1,711,862 - RR42_RS28305 0.68 +0.6
1,711,862 - RR42_RS28305 0.68 +0.2
1,711,981 + RR42_RS28305 0.76 +1.0
1,711,981 + RR42_RS28305 0.76 +0.2
1,712,017 - RR42_RS28305 0.79 +0.8
1,712,017 - RR42_RS28305 0.79 +1.4
1,712,017 - RR42_RS28305 0.79 -1.3
1,712,141 - RR42_RS28305 0.88 +0.3
1,712,221 + +1.3
1,712,221 + -1.5
1,712,221 + -0.2
1,712,613 + -1.9
1,712,617 + -0.2
1,712,618 - +0.7
1,712,618 - -0.2
1,712,848 + -0.7
1,712,889 + +0.5
1,713,010 - +1.3
1,713,097 + -1.7
1,713,099 + +0.9
1,713,100 - +0.2
1,713,112 - -0.1
1,713,124 - -1.2
1,713,124 - +0.4
1,713,185 + +1.1
1,713,186 - +1.0
1,713,186 - -1.4
1,713,193 + -0.0
1,713,193 + +0.6
1,713,307 - -3.6

Or see this region's nucleotide sequence