Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28240 and RR42_RS28245 are separated by 155 nucleotides RR42_RS28245 and RR42_RS28250 are separated by 155 nucleotides
RR42_RS28240: RR42_RS28240 - zinc carboxypeptidase, at 1,693,534 to 1,694,565
_RS28240
RR42_RS28245: RR42_RS28245 - phosphoserine phosphatase, at 1,694,721 to 1,695,419
_RS28245
RR42_RS28250: RR42_RS28250 - LysR family transcriptional regulator, at 1,695,575 to 1,696,444
_RS28250
Position (kb)
1694
1695
1696 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1693.848 kb on - strand, within RR42_RS28240 at 1693.875 kb on - strand, within RR42_RS28240 at 1693.875 kb on - strand, within RR42_RS28240 at 1693.875 kb on - strand, within RR42_RS28240 at 1693.992 kb on - strand, within RR42_RS28240 at 1694.331 kb on - strand, within RR42_RS28240 at 1694.432 kb on + strand, within RR42_RS28240 at 1694.433 kb on - strand, within RR42_RS28240 at 1694.625 kb on + strand at 1694.625 kb on + strand at 1694.625 kb on + strand at 1694.625 kb on + strand at 1694.625 kb on + strand at 1694.626 kb on - strand at 1694.626 kb on - strand at 1694.628 kb on - strand at 1694.876 kb on - strand, within RR42_RS28245 at 1694.876 kb on - strand, within RR42_RS28245 at 1694.976 kb on + strand, within RR42_RS28245 at 1695.277 kb on + strand, within RR42_RS28245 at 1695.346 kb on + strand, within RR42_RS28245 at 1695.362 kb on - strand at 1695.500 kb on - strand at 1695.628 kb on + strand at 1695.629 kb on - strand at 1695.629 kb on - strand at 1695.652 kb on - strand at 1695.652 kb on - strand at 1695.658 kb on - strand at 1695.771 kb on + strand, within RR42_RS28250 at 1695.771 kb on + strand, within RR42_RS28250 at 1695.772 kb on - strand, within RR42_RS28250 at 1695.772 kb on - strand, within RR42_RS28250 at 1696.125 kb on - strand, within RR42_RS28250 at 1696.125 kb on - strand, within RR42_RS28250 at 1696.140 kb on + strand, within RR42_RS28250 at 1696.140 kb on + strand, within RR42_RS28250 at 1696.140 kb on + strand, within RR42_RS28250 at 1696.140 kb on + strand, within RR42_RS28250 at 1696.141 kb on - strand, within RR42_RS28250 at 1696.141 kb on - strand, within RR42_RS28250 at 1696.317 kb on + strand, within RR42_RS28250 at 1696.318 kb on - strand, within RR42_RS28250 at 1696.416 kb on + strand at 1696.416 kb on + strand at 1696.416 kb on + strand at 1696.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 1,693,848 - RR42_RS28240 0.30 +0.2 1,693,875 - RR42_RS28240 0.33 +0.7 1,693,875 - RR42_RS28240 0.33 -2.0 1,693,875 - RR42_RS28240 0.33 -0.9 1,693,992 - RR42_RS28240 0.44 -0.7 1,694,331 - RR42_RS28240 0.77 +1.4 1,694,432 + RR42_RS28240 0.87 -0.1 1,694,433 - RR42_RS28240 0.87 -0.2 1,694,625 + +0.6 1,694,625 + +1.5 1,694,625 + -0.3 1,694,625 + -0.7 1,694,625 + +0.7 1,694,626 - -0.0 1,694,626 - +0.9 1,694,628 - +0.7 1,694,876 - RR42_RS28245 0.22 -0.5 1,694,876 - RR42_RS28245 0.22 -0.5 1,694,976 + RR42_RS28245 0.36 +0.6 1,695,277 + RR42_RS28245 0.80 +0.1 1,695,346 + RR42_RS28245 0.89 +0.4 1,695,362 - +0.7 1,695,500 - -0.1 1,695,628 + +0.5 1,695,629 - -0.1 1,695,629 - +0.8 1,695,652 - +0.7 1,695,652 - -1.7 1,695,658 - -0.6 1,695,771 + RR42_RS28250 0.23 +0.3 1,695,771 + RR42_RS28250 0.23 +0.1 1,695,772 - RR42_RS28250 0.23 -1.1 1,695,772 - RR42_RS28250 0.23 -0.6 1,696,125 - RR42_RS28250 0.63 -0.4 1,696,125 - RR42_RS28250 0.63 +0.1 1,696,140 + RR42_RS28250 0.65 -1.2 1,696,140 + RR42_RS28250 0.65 -0.0 1,696,140 + RR42_RS28250 0.65 -0.0 1,696,140 + RR42_RS28250 0.65 -0.8 1,696,141 - RR42_RS28250 0.65 +2.9 1,696,141 - RR42_RS28250 0.65 -1.6 1,696,317 + RR42_RS28250 0.85 -0.8 1,696,318 - RR42_RS28250 0.85 +0.9 1,696,416 + -0.7 1,696,416 + +0.1 1,696,416 + -0.1 1,696,417 - -1.5
Or see this region's nucleotide sequence