Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26550 and RR42_RS26555 are separated by 235 nucleotides RR42_RS26555 and RR42_RS26560 are separated by 269 nucleotides RR42_RS26560 and RR42_RS26565 are separated by 166 nucleotides RR42_RS26565 and RR42_RS26570 are separated by 148 nucleotides
RR42_RS26550: RR42_RS26550 - gluconolactonase, at 1,312,159 to 1,313,103
_RS26550
RR42_RS26555: RR42_RS26555 - tRNA-Met, at 1,313,339 to 1,313,414
_RS26555
RR42_RS26560: RR42_RS26560 - hypothetical protein, at 1,313,684 to 1,313,923
_RS26560
RR42_RS26565: RR42_RS26565 - 5'-3' exonuclease, at 1,314,090 to 1,314,896
_RS26565
RR42_RS26570: RR42_RS26570 - serine hydrolase, at 1,315,045 to 1,316,220
_RS26570
Position (kb) 1314
1315 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1313.321 kb on + strand at 1313.321 kb on + strand at 1313.378 kb on + strand, within RR42_RS26555 at 1313.425 kb on + strand at 1313.448 kb on + strand at 1313.464 kb on + strand at 1313.465 kb on - strand at 1313.582 kb on + strand at 1313.582 kb on + strand at 1313.850 kb on + strand, within RR42_RS26560 at 1313.850 kb on + strand, within RR42_RS26560 at 1313.851 kb on - strand, within RR42_RS26560 at 1313.851 kb on - strand, within RR42_RS26560 at 1313.851 kb on - strand, within RR42_RS26560 at 1313.891 kb on + strand, within RR42_RS26560 at 1313.892 kb on - strand, within RR42_RS26560 at 1313.933 kb on + strand at 1313.934 kb on - strand at 1313.934 kb on - strand at 1313.934 kb on - strand at 1314.041 kb on - strand at 1314.103 kb on + strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.295 kb on - strand, within RR42_RS26565 at 1314.295 kb on - strand, within RR42_RS26565 at 1314.411 kb on + strand, within RR42_RS26565 at 1314.444 kb on + strand, within RR42_RS26565 at 1314.445 kb on - strand, within RR42_RS26565 at 1314.478 kb on + strand, within RR42_RS26565 at 1314.589 kb on - strand, within RR42_RS26565 at 1314.589 kb on - strand, within RR42_RS26565 at 1314.634 kb on - strand, within RR42_RS26565 at 1314.653 kb on + strand, within RR42_RS26565 at 1314.665 kb on + strand, within RR42_RS26565 at 1314.666 kb on - strand, within RR42_RS26565 at 1314.921 kb on - strand at 1314.933 kb on + strand at 1315.071 kb on + strand at 1315.071 kb on - strand at 1315.071 kb on - strand at 1315.118 kb on + strand at 1315.118 kb on + strand at 1315.118 kb on + strand at 1315.119 kb on - strand at 1315.119 kb on - strand at 1315.119 kb on - strand at 1315.119 kb on - strand at 1315.119 kb on - strand at 1315.467 kb on - strand, within RR42_RS26570 at 1315.547 kb on + strand, within RR42_RS26570 at 1315.548 kb on - strand, within RR42_RS26570 at 1315.722 kb on - strand, within RR42_RS26570 at 1315.722 kb on - strand, within RR42_RS26570 at 1315.811 kb on + strand, within RR42_RS26570 at 1315.812 kb on - strand, within RR42_RS26570 at 1315.812 kb on - strand, within RR42_RS26570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 1,313,321 + +0.7 1,313,321 + -1.9 1,313,378 + RR42_RS26555 0.51 -1.2 1,313,425 + +1.6 1,313,448 + -1.0 1,313,464 + +1.1 1,313,465 - +0.6 1,313,582 + -2.4 1,313,582 + +0.4 1,313,850 + RR42_RS26560 0.69 +0.1 1,313,850 + RR42_RS26560 0.69 +0.5 1,313,851 - RR42_RS26560 0.70 +1.2 1,313,851 - RR42_RS26560 0.70 -0.4 1,313,851 - RR42_RS26560 0.70 -0.2 1,313,891 + RR42_RS26560 0.86 -1.3 1,313,892 - RR42_RS26560 0.87 +0.5 1,313,933 + +0.6 1,313,934 - -0.0 1,313,934 - +0.5 1,313,934 - +0.1 1,314,041 - -2.2 1,314,103 + -0.9 1,314,104 - +0.5 1,314,104 - -2.0 1,314,104 - +0.3 1,314,104 - +0.4 1,314,104 - +1.1 1,314,295 - RR42_RS26565 0.25 +1.6 1,314,295 - RR42_RS26565 0.25 -0.2 1,314,411 + RR42_RS26565 0.40 +0.4 1,314,444 + RR42_RS26565 0.44 +0.2 1,314,445 - RR42_RS26565 0.44 +0.1 1,314,478 + RR42_RS26565 0.48 -0.0 1,314,589 - RR42_RS26565 0.62 -1.1 1,314,589 - RR42_RS26565 0.62 +0.6 1,314,634 - RR42_RS26565 0.67 +0.7 1,314,653 + RR42_RS26565 0.70 -1.3 1,314,665 + RR42_RS26565 0.71 +0.6 1,314,666 - RR42_RS26565 0.71 +0.3 1,314,921 - +1.1 1,314,933 + +0.6 1,315,071 + +0.2 1,315,071 - -1.2 1,315,071 - -3.3 1,315,118 + -1.6 1,315,118 + +0.3 1,315,118 + +0.3 1,315,119 - +0.1 1,315,119 - -1.1 1,315,119 - +0.9 1,315,119 - -0.3 1,315,119 - -1.1 1,315,467 - RR42_RS26570 0.36 +0.5 1,315,547 + RR42_RS26570 0.43 -1.3 1,315,548 - RR42_RS26570 0.43 -1.0 1,315,722 - RR42_RS26570 0.58 -0.9 1,315,722 - RR42_RS26570 0.58 -0.5 1,315,811 + RR42_RS26570 0.65 -0.8 1,315,812 - RR42_RS26570 0.65 -1.8 1,315,812 - RR42_RS26570 0.65 +0.6
Or see this region's nucleotide sequence