Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25530

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25525 and RR42_RS25530 are separated by 158 nucleotidesRR42_RS25530 and RR42_RS25535 are separated by 132 nucleotides RR42_RS25525: RR42_RS25525 - enoyl-CoA hydratase, at 1,072,307 to 1,073,281 _RS25525 RR42_RS25530: RR42_RS25530 - TetR family transcriptional regulator, at 1,073,440 to 1,074,090 _RS25530 RR42_RS25535: RR42_RS25535 - TetR family transcriptional regulator, at 1,074,223 to 1,074,834 _RS25535 Position (kb) 1073 1074 1075Strain fitness (log2 ratio) -3 -2 -1 0 1at 1072.522 kb on - strand, within RR42_RS25525at 1072.573 kb on - strand, within RR42_RS25525at 1072.760 kb on + strand, within RR42_RS25525at 1072.760 kb on + strand, within RR42_RS25525at 1072.761 kb on - strand, within RR42_RS25525at 1072.807 kb on + strand, within RR42_RS25525at 1073.028 kb on - strand, within RR42_RS25525at 1073.111 kb on + strand, within RR42_RS25525at 1073.112 kb on - strand, within RR42_RS25525at 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.244 kb on - strandat 1073.409 kb on + strandat 1073.439 kb on + strandat 1073.440 kb on - strandat 1073.440 kb on - strandat 1073.451 kb on + strandat 1073.578 kb on + strand, within RR42_RS25530at 1073.578 kb on + strand, within RR42_RS25530at 1073.578 kb on + strand, within RR42_RS25530at 1073.579 kb on - strand, within RR42_RS25530at 1073.693 kb on + strand, within RR42_RS25530at 1073.693 kb on + strand, within RR42_RS25530at 1073.746 kb on + strand, within RR42_RS25530at 1073.782 kb on + strand, within RR42_RS25530at 1073.782 kb on + strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.784 kb on + strand, within RR42_RS25530at 1073.854 kb on + strand, within RR42_RS25530at 1073.855 kb on - strand, within RR42_RS25530at 1073.933 kb on + strand, within RR42_RS25530at 1073.933 kb on + strand, within RR42_RS25530at 1074.332 kb on - strand, within RR42_RS25535at 1074.392 kb on - strand, within RR42_RS25535at 1074.526 kb on + strand, within RR42_RS25535at 1074.526 kb on + strand, within RR42_RS25535at 1074.527 kb on - strand, within RR42_RS25535at 1074.527 kb on - strand, within RR42_RS25535at 1074.581 kb on + strand, within RR42_RS25535at 1074.582 kb on - strand, within RR42_RS25535at 1074.656 kb on + strand, within RR42_RS25535at 1074.656 kb on + strand, within RR42_RS25535at 1074.657 kb on - strand, within RR42_RS25535at 1074.657 kb on - strand, within RR42_RS25535at 1074.724 kb on - strand, within RR42_RS25535at 1074.724 kb on - strand, within RR42_RS25535at 1075.015 kb on - strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.081 kb on + strandat 1075.082 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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1,072,522 - RR42_RS25525 0.22 +0.3
1,072,573 - RR42_RS25525 0.27 +0.5
1,072,760 + RR42_RS25525 0.46 -0.6
1,072,760 + RR42_RS25525 0.46 +0.0
1,072,761 - RR42_RS25525 0.47 -1.1
1,072,807 + RR42_RS25525 0.51 -0.8
1,073,028 - RR42_RS25525 0.74 -0.9
1,073,111 + RR42_RS25525 0.82 +0.7
1,073,112 - RR42_RS25525 0.83 +0.7
1,073,243 + +0.3
1,073,243 + -0.6
1,073,243 + +0.4
1,073,244 - -3.6
1,073,409 + +0.6
1,073,439 + -0.2
1,073,440 - -0.1
1,073,440 - -0.2
1,073,451 + -2.8
1,073,578 + RR42_RS25530 0.21 +0.9
1,073,578 + RR42_RS25530 0.21 -2.3
1,073,578 + RR42_RS25530 0.21 -0.0
1,073,579 - RR42_RS25530 0.21 -0.5
1,073,693 + RR42_RS25530 0.39 -2.3
1,073,693 + RR42_RS25530 0.39 -0.8
1,073,746 + RR42_RS25530 0.47 -0.3
1,073,782 + RR42_RS25530 0.53 -0.5
1,073,782 + RR42_RS25530 0.53 -0.5
1,073,783 - RR42_RS25530 0.53 -0.8
1,073,783 - RR42_RS25530 0.53 +0.8
1,073,783 - RR42_RS25530 0.53 -1.9
1,073,783 - RR42_RS25530 0.53 -1.1
1,073,784 + RR42_RS25530 0.53 -0.5
1,073,854 + RR42_RS25530 0.64 -0.7
1,073,855 - RR42_RS25530 0.64 +0.1
1,073,933 + RR42_RS25530 0.76 -1.0
1,073,933 + RR42_RS25530 0.76 +0.1
1,074,332 - RR42_RS25535 0.18 +0.2
1,074,392 - RR42_RS25535 0.28 -1.1
1,074,526 + RR42_RS25535 0.50 -0.8
1,074,526 + RR42_RS25535 0.50 -2.1
1,074,527 - RR42_RS25535 0.50 -0.1
1,074,527 - RR42_RS25535 0.50 +0.8
1,074,581 + RR42_RS25535 0.58 -0.4
1,074,582 - RR42_RS25535 0.59 -0.6
1,074,656 + RR42_RS25535 0.71 -0.2
1,074,656 + RR42_RS25535 0.71 -1.2
1,074,657 - RR42_RS25535 0.71 -0.2
1,074,657 - RR42_RS25535 0.71 -0.1
1,074,724 - RR42_RS25535 0.82 -0.6
1,074,724 - RR42_RS25535 0.82 +0.4
1,075,015 - -3.3
1,075,081 + +0.8
1,075,081 + -0.1
1,075,081 + -0.4
1,075,081 + +0.7
1,075,081 + +1.2
1,075,082 - +0.2

Or see this region's nucleotide sequence