Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25175 and RR42_RS25180 are separated by 457 nucleotides RR42_RS25180 and RR42_RS25185 are separated by 226 nucleotides
RR42_RS25175: RR42_RS25175 - 3-keto-5-aminohexanoate cleavage protein, at 987,898 to 988,821
_RS25175
RR42_RS25180: RR42_RS25180 - AraC family transcriptional regulator, at 989,279 to 990,148
_RS25180
RR42_RS25185: RR42_RS25185 - epimerase, at 990,375 to 991,253
_RS25185
Position (kb) 989
990
991 Strain fitness (log2 ratio) -2
-1
0
1
2 at 988.348 kb on - strand, within RR42_RS25175 at 988.348 kb on - strand, within RR42_RS25175 at 988.405 kb on + strand, within RR42_RS25175 at 988.406 kb on - strand, within RR42_RS25175 at 988.406 kb on - strand, within RR42_RS25175 at 988.423 kb on + strand, within RR42_RS25175 at 989.100 kb on + strand at 989.129 kb on + strand at 989.293 kb on - strand at 989.319 kb on + strand at 989.568 kb on + strand, within RR42_RS25180 at 989.586 kb on - strand, within RR42_RS25180 at 989.694 kb on + strand, within RR42_RS25180 at 989.727 kb on - strand, within RR42_RS25180 at 989.727 kb on - strand, within RR42_RS25180 at 989.856 kb on - strand, within RR42_RS25180 at 989.856 kb on - strand, within RR42_RS25180 at 989.981 kb on + strand, within RR42_RS25180 at 990.173 kb on + strand at 990.174 kb on - strand at 990.245 kb on + strand at 990.306 kb on - strand at 990.340 kb on + strand at 990.388 kb on - strand at 990.584 kb on - strand, within RR42_RS25185 at 990.587 kb on + strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.715 kb on - strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.846 kb on - strand, within RR42_RS25185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 988,348 - RR42_RS25175 0.49 -0.6 988,348 - RR42_RS25175 0.49 +0.3 988,405 + RR42_RS25175 0.55 -1.0 988,406 - RR42_RS25175 0.55 -0.4 988,406 - RR42_RS25175 0.55 +0.3 988,423 + RR42_RS25175 0.57 +0.3 989,100 + -1.8 989,129 + -0.4 989,293 - -0.3 989,319 + +1.2 989,568 + RR42_RS25180 0.33 -1.3 989,586 - RR42_RS25180 0.35 -0.4 989,694 + RR42_RS25180 0.48 +0.7 989,727 - RR42_RS25180 0.51 -0.4 989,727 - RR42_RS25180 0.51 +0.6 989,856 - RR42_RS25180 0.66 +0.7 989,856 - RR42_RS25180 0.66 +1.4 989,981 + RR42_RS25180 0.81 +1.8 990,173 + +0.2 990,174 - -0.0 990,245 + +0.4 990,306 - -1.3 990,340 + +1.7 990,388 - -1.2 990,584 - RR42_RS25185 0.24 +0.7 990,587 + RR42_RS25185 0.24 +0.8 990,665 - RR42_RS25185 0.33 -0.5 990,665 - RR42_RS25185 0.33 +0.6 990,665 - RR42_RS25185 0.33 +1.6 990,715 - RR42_RS25185 0.39 +1.3 990,845 + RR42_RS25185 0.53 -1.8 990,845 + RR42_RS25185 0.53 -0.4 990,846 - RR42_RS25185 0.54 +0.8
Or see this region's nucleotide sequence