Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23220 and RR42_RS23225 are separated by 222 nucleotides RR42_RS23225 and RR42_RS23230 are separated by 215 nucleotides RR42_RS23230 and RR42_RS23235 are separated by 124 nucleotides
RR42_RS23220: RR42_RS23220 - MarR family transcriptional regulator, at 541,971 to 542,414
_RS23220
RR42_RS23225: RR42_RS23225 - hypothetical protein, at 542,637 to 542,948
_RS23225
RR42_RS23230: RR42_RS23230 - glycerate kinase, at 543,164 to 544,354
_RS23230
RR42_RS23235: RR42_RS23235 - tartronate semialdehyde reductase, at 544,479 to 545,339
_RS23235
Position (kb) 543
544
545 Strain fitness (log2 ratio) -2
-1
0
1 at 542.704 kb on + strand, within RR42_RS23225 at 542.704 kb on + strand, within RR42_RS23225 at 542.704 kb on + strand, within RR42_RS23225 at 542.705 kb on - strand, within RR42_RS23225 at 542.705 kb on - strand, within RR42_RS23225 at 542.705 kb on - strand, within RR42_RS23225 at 542.705 kb on - strand, within RR42_RS23225 at 542.705 kb on - strand, within RR42_RS23225 at 542.713 kb on + strand, within RR42_RS23225 at 542.763 kb on - strand, within RR42_RS23225 at 543.022 kb on + strand at 543.037 kb on + strand at 543.038 kb on - strand at 543.089 kb on + strand at 543.097 kb on + strand at 543.097 kb on + strand at 543.447 kb on - strand, within RR42_RS23230 at 543.519 kb on - strand, within RR42_RS23230 at 543.519 kb on - strand, within RR42_RS23230 at 544.348 kb on + strand at 544.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 542,704 + RR42_RS23225 0.21 +0.5 542,704 + RR42_RS23225 0.21 +0.5 542,704 + RR42_RS23225 0.21 +0.5 542,705 - RR42_RS23225 0.22 -1.9 542,705 - RR42_RS23225 0.22 +1.5 542,705 - RR42_RS23225 0.22 +0.4 542,705 - RR42_RS23225 0.22 +0.7 542,705 - RR42_RS23225 0.22 +0.4 542,713 + RR42_RS23225 0.24 +0.5 542,763 - RR42_RS23225 0.40 -0.1 543,022 + -0.4 543,037 + -1.6 543,038 - -1.2 543,089 + +0.3 543,097 + +0.4 543,097 + +0.2 543,447 - RR42_RS23230 0.24 +0.5 543,519 - RR42_RS23230 0.30 +0.2 543,519 - RR42_RS23230 0.30 -0.6 544,348 + -0.1 544,348 + +0.1
Or see this region's nucleotide sequence