Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22340 and RR42_RS22345 are separated by 233 nucleotides RR42_RS22345 and RR42_RS22350 are separated by 150 nucleotides RR42_RS22350 and RR42_RS22355 are separated by 211 nucleotides RR42_RS22355 and RR42_RS22360 are separated by 263 nucleotides
RR42_RS22340: RR42_RS22340 - peptidase, at 352,525 to 353,808
_RS22340
RR42_RS22345: RR42_RS22345 - UDP-N-acetylmuramate--alanine ligase, at 354,042 to 354,395
_RS22345
RR42_RS22350: RR42_RS22350 - transporter, at 354,546 to 354,974
_RS22350
RR42_RS22355: RR42_RS22355 - ModE family transcriptional regulator, at 355,186 to 355,512
_RS22355
RR42_RS22360: RR42_RS22360 - hypothetical protein, at 355,776 to 355,985
_RS22360
Position (kb) 354
355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 353.599 kb on + strand, within RR42_RS22340 at 353.638 kb on + strand, within RR42_RS22340 at 353.692 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.960 kb on - strand at 353.960 kb on - strand at 353.960 kb on - strand at 354.068 kb on - strand at 354.206 kb on + strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.318 kb on + strand, within RR42_RS22345 at 354.319 kb on - strand, within RR42_RS22345 at 354.381 kb on + strand at 354.382 kb on - strand at 354.458 kb on + strand at 354.459 kb on - strand at 354.578 kb on + strand at 355.025 kb on - strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.042 kb on - strand at 355.042 kb on - strand at 355.042 kb on - strand at 355.071 kb on - strand at 355.112 kb on - strand at 355.432 kb on + strand, within RR42_RS22355 at 355.449 kb on - strand, within RR42_RS22355 at 355.524 kb on + strand at 355.525 kb on - strand at 355.643 kb on - strand at 355.643 kb on - strand at 355.807 kb on + strand, within RR42_RS22360 at 355.807 kb on + strand, within RR42_RS22360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 353,599 + RR42_RS22340 0.84 +0.1 353,638 + RR42_RS22340 0.87 -1.1 353,692 + +0.1 353,959 + +1.2 353,959 + +0.3 353,959 + +0.4 353,959 + +0.6 353,960 - -0.3 353,960 - -1.1 353,960 - -0.4 354,068 - +2.1 354,206 + RR42_RS22345 0.46 -0.2 354,207 - RR42_RS22345 0.47 -0.2 354,207 - RR42_RS22345 0.47 +0.6 354,318 + RR42_RS22345 0.78 +2.5 354,319 - RR42_RS22345 0.78 -0.1 354,381 + -1.7 354,382 - -1.1 354,458 + +0.3 354,459 - -1.2 354,578 + -0.6 355,025 - -0.8 355,041 + -0.6 355,041 + -0.6 355,041 + +0.0 355,041 + -0.1 355,042 - -0.9 355,042 - +0.0 355,042 - -0.2 355,071 - +0.7 355,112 - -2.9 355,432 + RR42_RS22355 0.75 +0.4 355,449 - RR42_RS22355 0.80 -0.2 355,524 + -0.7 355,525 - +1.1 355,643 - -1.9 355,643 - +0.0 355,807 + RR42_RS22360 0.15 +1.1 355,807 + RR42_RS22360 0.15 +0.7
Or see this region's nucleotide sequence