Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22225 and RR42_RS22230 overlap by 4 nucleotides RR42_RS22230 and RR42_RS22235 are separated by 16 nucleotides RR42_RS22235 and RR42_RS22240 are separated by 821 nucleotides
RR42_RS22225: RR42_RS22225 - cytochrome C oxidase subunit III, at 324,981 to 325,388
_RS22225
RR42_RS22230: RR42_RS22230 - hypothetical protein, at 325,385 to 326,239
_RS22230
RR42_RS22235: RR42_RS22235 - peptidase M19, at 326,256 to 326,885
_RS22235
RR42_RS22240: RR42_RS22240 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 327,707 to 329,008
_RS22240
Position (kb) 326
327 Strain fitness (log2 ratio) -2
-1
0
1
2 at 325.976 kb on + strand, within RR42_RS22230 at 326.082 kb on - strand, within RR42_RS22230 at 326.133 kb on - strand, within RR42_RS22230 at 326.173 kb on + strand at 326.371 kb on + strand, within RR42_RS22235 at 326.372 kb on - strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.659 kb on + strand, within RR42_RS22235 at 326.660 kb on - strand, within RR42_RS22235 at 326.660 kb on - strand, within RR42_RS22235 at 326.827 kb on + strand at 326.828 kb on - strand at 326.828 kb on - strand at 327.005 kb on - strand at 327.651 kb on + strand at 327.652 kb on - strand at 327.652 kb on - strand at 327.697 kb on - strand at 327.751 kb on + strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.752 kb on - strand at 327.755 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 325,976 + RR42_RS22230 0.69 +0.3 326,082 - RR42_RS22230 0.82 +0.1 326,133 - RR42_RS22230 0.87 -2.1 326,173 + +1.1 326,371 + RR42_RS22235 0.18 -0.3 326,372 - RR42_RS22235 0.18 -0.1 326,659 + RR42_RS22235 0.64 +0.7 326,659 + RR42_RS22235 0.64 -0.0 326,659 + RR42_RS22235 0.64 +0.9 326,659 + RR42_RS22235 0.64 -0.0 326,660 - RR42_RS22235 0.64 +2.1 326,660 - RR42_RS22235 0.64 +0.3 326,827 + -1.7 326,828 - -0.3 326,828 - +0.1 327,005 - -1.3 327,651 + +0.1 327,652 - -1.0 327,652 - -0.3 327,697 - +0.3 327,751 + -1.9 327,752 - +0.6 327,752 - -0.8 327,752 - +0.7 327,755 - +1.5
Or see this region's nucleotide sequence