Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21115 and RR42_RS21120 are separated by 137 nucleotides RR42_RS21120 and RR42_RS21125 are separated by 249 nucleotides
RR42_RS21115: RR42_RS21115 - flagellin, at 62,515 to 63,195
_RS21115
RR42_RS21120: RR42_RS21120 - acetate kinase, at 63,333 to 64,745
_RS21120
RR42_RS21125: RR42_RS21125 - membrane protein, at 64,995 to 65,507
_RS21125
Position (kb) 63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 62.382 kb on - strand at 62.492 kb on + strand at 62.493 kb on - strand at 62.703 kb on + strand, within RR42_RS21115 at 62.703 kb on + strand, within RR42_RS21115 at 62.704 kb on - strand, within RR42_RS21115 at 62.704 kb on - strand, within RR42_RS21115 at 62.704 kb on - strand, within RR42_RS21115 at 62.706 kb on + strand, within RR42_RS21115 at 62.706 kb on + strand, within RR42_RS21115 at 62.707 kb on - strand, within RR42_RS21115 at 62.707 kb on - strand, within RR42_RS21115 at 62.851 kb on - strand, within RR42_RS21115 at 62.871 kb on + strand, within RR42_RS21115 at 62.872 kb on - strand, within RR42_RS21115 at 62.872 kb on - strand, within RR42_RS21115 at 62.872 kb on - strand, within RR42_RS21115 at 62.993 kb on + strand, within RR42_RS21115 at 62.993 kb on + strand, within RR42_RS21115 at 62.993 kb on + strand, within RR42_RS21115 at 63.171 kb on - strand at 63.201 kb on - strand at 63.201 kb on - strand at 63.239 kb on - strand at 63.241 kb on + strand at 63.241 kb on + strand at 63.241 kb on + strand at 63.241 kb on + strand at 63.245 kb on + strand at 63.245 kb on + strand at 63.245 kb on + strand at 63.246 kb on - strand at 63.246 kb on - strand at 63.269 kb on + strand at 63.270 kb on - strand at 63.880 kb on - strand, within RR42_RS21120 at 63.880 kb on - strand, within RR42_RS21120 at 63.975 kb on + strand, within RR42_RS21120 at 63.975 kb on + strand, within RR42_RS21120 at 63.975 kb on + strand, within RR42_RS21120 at 63.976 kb on - strand, within RR42_RS21120 at 63.976 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.042 kb on - strand, within RR42_RS21120 at 64.155 kb on + strand, within RR42_RS21120 at 64.167 kb on + strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.209 kb on + strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.347 kb on - strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.635 kb on + strand at 64.660 kb on - strand at 64.827 kb on - strand at 64.827 kb on - strand at 64.842 kb on + strand at 64.843 kb on - strand at 65.006 kb on + strand at 65.006 kb on + strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.117 kb on + strand, within RR42_RS21125 at 65.118 kb on - strand, within RR42_RS21125 at 65.614 kb on - strand at 65.681 kb on + strand at 65.682 kb on - strand at 65.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 62,382 - +0.3 62,492 + -1.0 62,493 - -0.2 62,703 + RR42_RS21115 0.28 +0.8 62,703 + RR42_RS21115 0.28 -0.6 62,704 - RR42_RS21115 0.28 +1.0 62,704 - RR42_RS21115 0.28 +0.5 62,704 - RR42_RS21115 0.28 -1.8 62,706 + RR42_RS21115 0.28 +0.8 62,706 + RR42_RS21115 0.28 +0.0 62,707 - RR42_RS21115 0.28 +0.4 62,707 - RR42_RS21115 0.28 -0.0 62,851 - RR42_RS21115 0.49 +0.1 62,871 + RR42_RS21115 0.52 +1.1 62,872 - RR42_RS21115 0.52 -0.0 62,872 - RR42_RS21115 0.52 -0.2 62,872 - RR42_RS21115 0.52 -0.1 62,993 + RR42_RS21115 0.70 +1.1 62,993 + RR42_RS21115 0.70 -0.2 62,993 + RR42_RS21115 0.70 +0.2 63,171 - -0.5 63,201 - +0.2 63,201 - -0.6 63,239 - +1.1 63,241 + -1.3 63,241 + +1.1 63,241 + -2.3 63,241 + +0.3 63,245 + +1.2 63,245 + -0.1 63,245 + +0.9 63,246 - -0.6 63,246 - +0.6 63,269 + +0.3 63,270 - -0.4 63,880 - RR42_RS21120 0.39 +0.1 63,880 - RR42_RS21120 0.39 -0.1 63,975 + RR42_RS21120 0.45 -1.6 63,975 + RR42_RS21120 0.45 +1.5 63,975 + RR42_RS21120 0.45 +1.7 63,976 - RR42_RS21120 0.46 -3.1 63,976 - RR42_RS21120 0.46 -1.8 64,025 - RR42_RS21120 0.49 +0.1 64,025 - RR42_RS21120 0.49 -1.2 64,025 - RR42_RS21120 0.49 -1.0 64,025 - RR42_RS21120 0.49 +1.3 64,042 - RR42_RS21120 0.50 -0.6 64,155 + RR42_RS21120 0.58 +1.4 64,167 + RR42_RS21120 0.59 -1.3 64,168 - RR42_RS21120 0.59 +0.9 64,168 - RR42_RS21120 0.59 -1.3 64,209 + RR42_RS21120 0.62 -1.6 64,210 - RR42_RS21120 0.62 -0.3 64,210 - RR42_RS21120 0.62 -1.6 64,347 - RR42_RS21120 0.72 -1.0 64,395 + RR42_RS21120 0.75 +1.2 64,395 + RR42_RS21120 0.75 -2.4 64,395 + RR42_RS21120 0.75 +0.1 64,635 + +0.7 64,660 - +0.4 64,827 - +0.5 64,827 - -1.0 64,842 + -0.2 64,843 - -0.7 65,006 + -1.2 65,006 + -0.4 65,007 - +0.4 65,007 - -0.7 65,007 - +0.4 65,117 + RR42_RS21125 0.24 -0.4 65,118 - RR42_RS21125 0.24 -0.7 65,614 - +0.6 65,681 + -1.3 65,682 - -0.3 65,682 - -0.6
Or see this region's nucleotide sequence