Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20060 and RR42_RS20065 are separated by 102 nucleotides RR42_RS20065 and RR42_RS20070 are separated by 101 nucleotides
RR42_RS20060: RR42_RS20060 - glycine cleavage system protein T, at 4,334,296 to 4,335,435
_RS20060
RR42_RS20065: RR42_RS20065 - glycine cleavage system protein H, at 4,335,538 to 4,335,918
_RS20065
RR42_RS20070: RR42_RS20070 - glycine dehydrogenase, at 4,336,020 to 4,338,941
_RS20070
Position (kb) 4335
4336 Strain fitness (log2 ratio) -2
-1
0
1 at 4334.551 kb on + strand, within RR42_RS20060 at 4334.551 kb on + strand, within RR42_RS20060 at 4334.551 kb on + strand, within RR42_RS20060 at 4334.552 kb on - strand, within RR42_RS20060 at 4334.552 kb on - strand, within RR42_RS20060 at 4334.661 kb on + strand, within RR42_RS20060 at 4334.661 kb on + strand, within RR42_RS20060 at 4334.662 kb on - strand, within RR42_RS20060 at 4334.697 kb on + strand, within RR42_RS20060 at 4334.698 kb on - strand, within RR42_RS20060 at 4334.908 kb on - strand, within RR42_RS20060 at 4335.047 kb on - strand, within RR42_RS20060 at 4335.394 kb on - strand at 4335.562 kb on + strand at 4335.562 kb on + strand at 4335.563 kb on - strand at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.746 kb on - strand, within RR42_RS20065 at 4335.895 kb on + strand at 4335.895 kb on + strand at 4335.895 kb on + strand at 4335.895 kb on + strand at 4335.896 kb on - strand at 4335.897 kb on + strand at 4335.897 kb on + strand at 4335.897 kb on + strand at 4336.330 kb on - strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.333 kb on - strand, within RR42_RS20070 at 4336.333 kb on - strand, within RR42_RS20070 at 4336.333 kb on - strand, within RR42_RS20070 at 4336.390 kb on - strand, within RR42_RS20070 at 4336.399 kb on - strand, within RR42_RS20070 at 4336.581 kb on + strand, within RR42_RS20070 at 4336.581 kb on + strand, within RR42_RS20070 at 4336.582 kb on - strand, within RR42_RS20070 at 4336.621 kb on + strand, within RR42_RS20070 at 4336.914 kb on + strand, within RR42_RS20070 at 4336.915 kb on - strand, within RR42_RS20070 at 4336.915 kb on - strand, within RR42_RS20070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 4,334,551 + RR42_RS20060 0.22 -1.3 4,334,551 + RR42_RS20060 0.22 -0.8 4,334,551 + RR42_RS20060 0.22 -0.4 4,334,552 - RR42_RS20060 0.22 -0.1 4,334,552 - RR42_RS20060 0.22 -1.1 4,334,661 + RR42_RS20060 0.32 -1.8 4,334,661 + RR42_RS20060 0.32 -1.2 4,334,662 - RR42_RS20060 0.32 -0.2 4,334,697 + RR42_RS20060 0.35 -0.9 4,334,698 - RR42_RS20060 0.35 -0.3 4,334,908 - RR42_RS20060 0.54 -1.9 4,335,047 - RR42_RS20060 0.66 -1.2 4,335,394 - -0.5 4,335,562 + -1.3 4,335,562 + +0.8 4,335,563 - -2.1 4,335,745 + RR42_RS20065 0.54 -0.5 4,335,745 + RR42_RS20065 0.54 -1.4 4,335,745 + RR42_RS20065 0.54 -1.9 4,335,745 + RR42_RS20065 0.54 +0.2 4,335,745 + RR42_RS20065 0.54 -0.4 4,335,746 - RR42_RS20065 0.55 +0.7 4,335,895 + +0.5 4,335,895 + -1.2 4,335,895 + -1.3 4,335,895 + -0.1 4,335,896 - +0.1 4,335,897 + -0.5 4,335,897 + +0.1 4,335,897 + -2.1 4,336,330 - RR42_RS20070 0.11 +0.6 4,336,332 + RR42_RS20070 0.11 -0.4 4,336,332 + RR42_RS20070 0.11 +0.4 4,336,332 + RR42_RS20070 0.11 +1.0 4,336,332 + RR42_RS20070 0.11 +0.7 4,336,332 + RR42_RS20070 0.11 +0.2 4,336,332 + RR42_RS20070 0.11 +0.4 4,336,333 - RR42_RS20070 0.11 -0.2 4,336,333 - RR42_RS20070 0.11 +1.4 4,336,333 - RR42_RS20070 0.11 +1.1 4,336,390 - RR42_RS20070 0.13 +0.5 4,336,399 - RR42_RS20070 0.13 +1.1 4,336,581 + RR42_RS20070 0.19 +1.0 4,336,581 + RR42_RS20070 0.19 +0.0 4,336,582 - RR42_RS20070 0.19 -0.9 4,336,621 + RR42_RS20070 0.21 -0.6 4,336,914 + RR42_RS20070 0.31 +1.5 4,336,915 - RR42_RS20070 0.31 +0.4 4,336,915 - RR42_RS20070 0.31 +1.0
Or see this region's nucleotide sequence