Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18485 and RR42_RS18490 are separated by 7 nucleotides RR42_RS18490 and RR42_RS18495 are separated by 54 nucleotides RR42_RS18495 and RR42_RS18500 are separated by 151 nucleotides
RR42_RS18485: RR42_RS18485 - glutaredoxin, at 4,001,845 to 4,002,156
_RS18485
RR42_RS18490: RR42_RS18490 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 4,002,164 to 4,002,772
_RS18490
RR42_RS18495: RR42_RS18495 - hypothetical protein, at 4,002,827 to 4,003,054
_RS18495
RR42_RS18500: RR42_RS18500 - aldehyde dehydrogenase, at 4,003,206 to 4,004,684
_RS18500
Position (kb)
4002
4003
4004 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4002.118 kb on + strand, within RR42_RS18485 at 4002.784 kb on + strand at 4002.784 kb on + strand at 4002.785 kb on - strand at 4002.785 kb on - strand at 4002.804 kb on + strand at 4002.858 kb on + strand, within RR42_RS18495 at 4002.858 kb on + strand, within RR42_RS18495 at 4002.859 kb on - strand, within RR42_RS18495 at 4002.859 kb on - strand, within RR42_RS18495 at 4002.861 kb on + strand, within RR42_RS18495 at 4002.874 kb on - strand, within RR42_RS18495 at 4002.879 kb on + strand, within RR42_RS18495 at 4002.879 kb on + strand, within RR42_RS18495 at 4002.879 kb on + strand, within RR42_RS18495 at 4002.879 kb on + strand, within RR42_RS18495 at 4002.879 kb on + strand, within RR42_RS18495 at 4002.879 kb on + strand, within RR42_RS18495 at 4002.880 kb on - strand, within RR42_RS18495 at 4002.880 kb on - strand, within RR42_RS18495 at 4002.880 kb on - strand, within RR42_RS18495 at 4002.880 kb on - strand, within RR42_RS18495 at 4002.882 kb on + strand, within RR42_RS18495 at 4002.882 kb on + strand, within RR42_RS18495 at 4002.882 kb on + strand, within RR42_RS18495 at 4002.882 kb on + strand, within RR42_RS18495 at 4002.882 kb on + strand, within RR42_RS18495 at 4002.883 kb on - strand, within RR42_RS18495 at 4002.883 kb on - strand, within RR42_RS18495 at 4002.891 kb on + strand, within RR42_RS18495 at 4002.892 kb on - strand, within RR42_RS18495 at 4002.927 kb on + strand, within RR42_RS18495 at 4002.966 kb on + strand, within RR42_RS18495 at 4002.972 kb on + strand, within RR42_RS18495 at 4003.077 kb on + strand at 4003.095 kb on - strand at 4003.103 kb on + strand at 4003.104 kb on - strand at 4003.104 kb on - strand at 4003.105 kb on + strand at 4003.105 kb on + strand at 4003.106 kb on - strand at 4003.106 kb on - strand at 4003.121 kb on - strand at 4003.121 kb on - strand at 4003.124 kb on - strand at 4003.141 kb on + strand at 4003.141 kb on + strand at 4003.141 kb on + strand at 4003.152 kb on - strand at 4003.177 kb on + strand at 4003.178 kb on - strand at 4003.178 kb on - strand at 4003.252 kb on + strand at 4003.253 kb on - strand at 4003.263 kb on - strand at 4003.280 kb on + strand at 4003.280 kb on + strand at 4003.281 kb on - strand at 4003.281 kb on - strand at 4003.282 kb on + strand at 4003.282 kb on + strand at 4003.282 kb on + strand at 4003.283 kb on - strand at 4003.283 kb on - strand at 4003.516 kb on + strand, within RR42_RS18500 at 4003.517 kb on - strand, within RR42_RS18500 at 4003.528 kb on + strand, within RR42_RS18500 at 4003.528 kb on + strand, within RR42_RS18500 at 4003.603 kb on + strand, within RR42_RS18500 at 4003.603 kb on + strand, within RR42_RS18500 at 4003.797 kb on - strand, within RR42_RS18500 at 4003.797 kb on - strand, within RR42_RS18500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 4,002,118 + RR42_RS18485 0.88 +1.2 4,002,784 + +0.6 4,002,784 + +1.0 4,002,785 - +0.3 4,002,785 - -0.1 4,002,804 + +0.2 4,002,858 + RR42_RS18495 0.14 -2.1 4,002,858 + RR42_RS18495 0.14 -0.0 4,002,859 - RR42_RS18495 0.14 -0.0 4,002,859 - RR42_RS18495 0.14 -3.5 4,002,861 + RR42_RS18495 0.15 +0.4 4,002,874 - RR42_RS18495 0.21 +0.3 4,002,879 + RR42_RS18495 0.23 +0.3 4,002,879 + RR42_RS18495 0.23 +0.2 4,002,879 + RR42_RS18495 0.23 -0.4 4,002,879 + RR42_RS18495 0.23 -2.1 4,002,879 + RR42_RS18495 0.23 -0.0 4,002,879 + RR42_RS18495 0.23 -0.3 4,002,880 - RR42_RS18495 0.23 -3.1 4,002,880 - RR42_RS18495 0.23 +1.0 4,002,880 - RR42_RS18495 0.23 +0.2 4,002,880 - RR42_RS18495 0.23 -1.6 4,002,882 + RR42_RS18495 0.24 -1.1 4,002,882 + RR42_RS18495 0.24 +1.6 4,002,882 + RR42_RS18495 0.24 +0.2 4,002,882 + RR42_RS18495 0.24 -0.4 4,002,882 + RR42_RS18495 0.24 -0.8 4,002,883 - RR42_RS18495 0.25 +1.3 4,002,883 - RR42_RS18495 0.25 -0.6 4,002,891 + RR42_RS18495 0.28 +0.6 4,002,892 - RR42_RS18495 0.29 -0.6 4,002,927 + RR42_RS18495 0.44 -2.0 4,002,966 + RR42_RS18495 0.61 +0.7 4,002,972 + RR42_RS18495 0.64 +0.1 4,003,077 + +0.4 4,003,095 - +1.4 4,003,103 + +0.3 4,003,104 - -0.5 4,003,104 - +0.3 4,003,105 + -0.0 4,003,105 + -2.1 4,003,106 - +0.6 4,003,106 - +0.3 4,003,121 - +0.0 4,003,121 - +0.1 4,003,124 - +0.0 4,003,141 + -0.8 4,003,141 + -2.4 4,003,141 + +1.0 4,003,152 - -0.8 4,003,177 + +0.3 4,003,178 - -0.1 4,003,178 - +1.3 4,003,252 + -0.4 4,003,253 - +0.1 4,003,263 - -0.3 4,003,280 + +0.8 4,003,280 + -0.3 4,003,281 - -0.3 4,003,281 - -2.3 4,003,282 + +1.1 4,003,282 + -1.9 4,003,282 + +0.4 4,003,283 - -0.3 4,003,283 - +0.1 4,003,516 + RR42_RS18500 0.21 -0.3 4,003,517 - RR42_RS18500 0.21 +0.4 4,003,528 + RR42_RS18500 0.22 +0.3 4,003,528 + RR42_RS18500 0.22 -0.6 4,003,603 + RR42_RS18500 0.27 +0.2 4,003,603 + RR42_RS18500 0.27 +0.5 4,003,797 - RR42_RS18500 0.40 +0.6 4,003,797 - RR42_RS18500 0.40 -1.1
Or see this region's nucleotide sequence