Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17915 and RR42_RS17920 overlap by 4 nucleotides RR42_RS17920 and RR42_RS17925 are separated by 23 nucleotides RR42_RS17925 and RR42_RS17930 are separated by 185 nucleotides RR42_RS17930 and RR42_RS17935 overlap by 8 nucleotides
RR42_RS17915: RR42_RS17915 - proline dioxygenase, at 3,876,751 to 3,877,701
_RS17915
RR42_RS17920: RR42_RS17920 - lytic transglycosylase, at 3,877,698 to 3,878,444
_RS17920
RR42_RS17925: RR42_RS17925 - prolyl-tRNA synthetase, at 3,878,468 to 3,880,189
_RS17925
RR42_RS17930: RR42_RS17930 - RNA pyrophosphohydrolase, at 3,880,375 to 3,881,037
_RS17930
RR42_RS17935: RR42_RS17935 - lipoprotein, at 3,881,030 to 3,881,659
_RS17935
Position (kb)
3878
3879
3880
3881 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3877.655 kb on + strand at 3877.865 kb on - strand, within RR42_RS17920 at 3877.891 kb on + strand, within RR42_RS17920 at 3877.892 kb on - strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.936 kb on + strand, within RR42_RS17920 at 3877.937 kb on - strand, within RR42_RS17920 at 3877.937 kb on - strand, within RR42_RS17920 at 3877.960 kb on + strand, within RR42_RS17920 at 3878.034 kb on + strand, within RR42_RS17920 at 3878.034 kb on + strand, within RR42_RS17920 at 3878.034 kb on + strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.035 kb on - strand, within RR42_RS17920 at 3878.075 kb on - strand, within RR42_RS17920 at 3878.075 kb on - strand, within RR42_RS17920 at 3878.075 kb on - strand, within RR42_RS17920 at 3878.149 kb on + strand at 3878.149 kb on + strand, within RR42_RS17920 at 3878.149 kb on + strand, within RR42_RS17920 at 3878.149 kb on + strand, within RR42_RS17920 at 3878.150 kb on - strand, within RR42_RS17920 at 3878.150 kb on - strand, within RR42_RS17920 at 3878.150 kb on - strand, within RR42_RS17920 at 3878.152 kb on + strand, within RR42_RS17920 at 3878.152 kb on + strand, within RR42_RS17920 at 3878.239 kb on + strand, within RR42_RS17920 at 3880.338 kb on - strand at 3880.338 kb on - strand at 3880.338 kb on - strand at 3880.535 kb on + strand, within RR42_RS17930 at 3880.536 kb on - strand, within RR42_RS17930 at 3880.536 kb on - strand, within RR42_RS17930 at 3880.536 kb on - strand, within RR42_RS17930 at 3880.649 kb on - strand, within RR42_RS17930 at 3880.759 kb on + strand, within RR42_RS17930 at 3880.759 kb on + strand, within RR42_RS17930 at 3880.805 kb on - strand, within RR42_RS17930 at 3880.848 kb on + strand, within RR42_RS17930 at 3880.909 kb on + strand, within RR42_RS17930 at 3881.037 kb on - strand at 3881.171 kb on + strand, within RR42_RS17935 at 3881.172 kb on - strand, within RR42_RS17935
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 3,877,655 + +0.4 3,877,865 - RR42_RS17920 0.22 +0.4 3,877,891 + RR42_RS17920 0.26 +1.3 3,877,892 - RR42_RS17920 0.26 +1.1 3,877,936 + RR42_RS17920 0.32 +0.3 3,877,936 + RR42_RS17920 0.32 +0.2 3,877,936 + RR42_RS17920 0.32 +0.9 3,877,936 + RR42_RS17920 0.32 +0.7 3,877,937 - RR42_RS17920 0.32 +1.0 3,877,937 - RR42_RS17920 0.32 +0.3 3,877,960 + RR42_RS17920 0.35 -1.6 3,878,034 + RR42_RS17920 0.45 +0.6 3,878,034 + RR42_RS17920 0.45 -1.7 3,878,034 + RR42_RS17920 0.45 -1.0 3,878,035 - RR42_RS17920 0.45 +0.3 3,878,035 - RR42_RS17920 0.45 -0.4 3,878,035 - RR42_RS17920 0.45 +0.4 3,878,035 - RR42_RS17920 0.45 +0.9 3,878,035 - RR42_RS17920 0.45 -0.6 3,878,075 - RR42_RS17920 0.50 +2.1 3,878,075 - RR42_RS17920 0.50 -0.2 3,878,075 - RR42_RS17920 0.50 +0.4 3,878,149 + -0.2 3,878,149 + RR42_RS17920 0.60 +0.8 3,878,149 + RR42_RS17920 0.60 +1.0 3,878,149 + RR42_RS17920 0.60 +0.8 3,878,150 - RR42_RS17920 0.61 +0.6 3,878,150 - RR42_RS17920 0.61 -1.1 3,878,150 - RR42_RS17920 0.61 +0.4 3,878,152 + RR42_RS17920 0.61 +0.4 3,878,152 + RR42_RS17920 0.61 +1.5 3,878,239 + RR42_RS17920 0.72 +0.0 3,880,338 - +1.8 3,880,338 - +1.7 3,880,338 - +1.0 3,880,535 + RR42_RS17930 0.24 +0.8 3,880,536 - RR42_RS17930 0.24 +1.0 3,880,536 - RR42_RS17930 0.24 +2.2 3,880,536 - RR42_RS17930 0.24 +2.0 3,880,649 - RR42_RS17930 0.41 +2.5 3,880,759 + RR42_RS17930 0.58 +0.2 3,880,759 + RR42_RS17930 0.58 +0.9 3,880,805 - RR42_RS17930 0.65 +1.7 3,880,848 + RR42_RS17930 0.71 +0.7 3,880,909 + RR42_RS17930 0.81 +3.1 3,881,037 - +1.7 3,881,171 + RR42_RS17935 0.22 +1.6 3,881,172 - RR42_RS17935 0.23 +0.6
Or see this region's nucleotide sequence